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Semaphorin 4D (SEMA4D) (NM_006378) Human Untagged Clone
CAT＃: SC116159
SEMA4D (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1
CNY 9,480.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A8; BB18; C9orf164; CD100; coll-4; COLL4; GR3; M-sema-G; SEMAJ |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116159 sequence for NM_006378 edited (data generated by NextGen Sequencing)
ATGAGGATGTGCACCCCCATTAGGGGGCTGCTCATGGCCCTTGCAGTGATGTTTGGGACA GCGATGGCATTTGCACCCATACCCCGGATCACCTGGGAGCACAGAGAGGTGCACCTGGTG CAGTTTCATGAGCCAGACATCTACAACTACTCAGCCTTGCTGCTGAGCGAGGACAAGGAC ACCTTGTACATAGGTGCCCGGGAGGCGGTCTTCGCTGTGAACGCACTCAACATCTCCGAG AAGCAGCATGAGGTGTATTGGAAGGTCTCAGAAGACAAAAAAGCAAAATGTGCAGAAAAG GGGAAATCAAAACAGACAGAGTGCCTCAACTACATCCGGGTGCTGCAGCCACTCAGCGCC ACTTCCCTTTACGTGTGTGGGACCAACGCATTCCAGCCGGCCTGTGACCACCTGAACTTA ACATCCTTTAAGTTTCTGGGGAAAAATGAAGATGGCAAAGGAAGATGTCCCTTTGACCCA GCACACAGCTACACATCCGTCATGGTTGATGGAGAACTTTATTCGGGGACGTCGTATAAT TTTTTGGGAAGTGAACCCATCATCTCCCGAAATTCTTCCCACAGTCCTCTGAGGACAGAA TATGCAATCCCTTGGCTGAACGAGCCTAGTTTCGTGTTTGCTGACGTGATCCGAAAAAGC CCAGACAGCCCCGACGGCGAGGATGACAGGGTCTACTTCTTCTTCACGGAGGTGTCTGTG GAGTATGAGTTTGTGTTCAGGGTGCTGATCCCACGGATAGCAAGAGTGTGCAAGGGGGAC CAGGGCGGCCTGAGGACCTTGCAGAAGAAATGGACCTCCTTCCTGAAAGCCCGACTCATC TGCTCCCGGCCAGACAGCGGCTTGGTCTTCAATGTGCTGCGGGATGTCTTCGTGCTCAGG TCCCCGGGCCTGAAGGTGCCTGTGTTCTATGCACTCTTCACCCCACAGCTGAACAACGTG GGGCTGTCGGCAGTGTGCACCTACAACCTGTCCACAGCCGAGGAGGTCTTCTCCCACGGG AAGTACATGCAGAGCACCACAGTGGAGCAGTCCCACACCAAGTGGGTGCGCTATAATGGC CCGGTACCCAAGCCGCGGCCTGGAGCGTGCATCGACAGCGAGGCACGGGCCGCCAACTAC ACCAGCTCCTTGAATTTGCCAGACAAGACGCTGCAGTTCGTTAAAGACCACCCTTTGATG GATGACTCGGTAACCCCAATAGACAACAGGCCCAGGTTAATCAAGAAAGATGTGAACTAC ACCCAGATCGTGGTGGACCGGACCCAGGCCCTGGATGGGACTGTCTATGATGTCATGTTT GTCAGCACAGACCGGGGAGCTCTGCACAAAGCCATCAGCCTCGAGCACGCTGTTCACATC ATCGAGGAGACCCAGCTCTTCCAGGACTTTGAGCCAGTCCAGACCCTGCTGCTGTCTTCA AAGAAGGGCAACAGGTTTGTCTATGCTGGCTCTAACTCGGGCGTGGTCCAGGCCCCGCTG GCCTTCTGTGGGAAGCACGGCACCTGCGAGGACTGTGTGCTGGCGCGGGACCCCTACTGC GCCTGGAGCCCGCCCACAGCGACCTGCGTGGCTCTGCACCAGACCGAGAGCCCCAGCAGG GGTTTGATTCAGGAGATGAGCGGCGATGCTTCTGTGTGCCCGGATAAAAGTAAAGGAAGT TACCGGCAGCATTTTTTCAAGCACGGTGGCACAGCGGAACTGAAATGCTCCCAAAAATCC AACCTGGCCCGGGTCTTTTGGAAGTTCCAGAATGGCGTGTTGAAGGCCGAGAGCCCCAAG TACGGTCTTATGGGCAGAAAAAACTTGCTCATCTTCAACTTGTCAGAAGGAGACAGTGGG GTGTACCAGTGCCTGTCAGAGGAGAGGGTTAAGAACAAAACGGTCTTCCAAGTGGTCGCC AAGCACGTCCTGGAAGTGAAGGTGGTTCCAAAGCCCGTAGTGGCCCCCACCTTGTCAGTT GTTCAGACAGAAGGTAGTAGGATTGCCACCAAAGTGTTGGTGGCATCCACCCAAGGGTCT TCTCCCCCAACCCCAGCCGTGCAGGCCACCTCCTCCGGGGCCATCACCCTTCCTCCCAAG CCTGCGCCCACCGGCACATCCTGCGAACCAAAGATCGTCATCAACACGGTCCCCCAGCTC CACTCGGAGAAAACCATGTATCTTAAGTCCAGCGACAACCGCCTCCTCATGTCCCTCTTC CTCTTCTTCTTTGTTCTCTTCCTCTGCCTCTTTTTCTACAACTGCTATAAGGGATACCTG CCCAGACAGTGCTTGAAATTCCGCTCGGCCCTACTAATTGGGAAGAAGAAGCCCAAGTCA GATTTCTGTGACCGTGAGCAGAGCCTGAAGGAGACGTTAGTAGAGCCAGGGAGCTTCTCC CAGCAGAATGGGGAGCACCCCAAGCCAGCCCTGGACACCGGCTATGAGACCGAGCAAGAC ACCATCACCAGCAAAGTCCCCACGGATAGGGAGGACTCACAGAGGATCGACGACCTTTCT GCCAGGGACAAGCCCTTTGACGTCAAGTGTGAGCTGAAGTTCGCTGACTCAGACGCAGAT GGAGACTGA Clone variation with respect to NM_006378.3 979 g=>a >OriGene 5' read for NM_006378 unedited
AGAATTTATGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGCGCATCTGCA ATAGCACACTTGCCCGGCCACCTGCTGCCGTGAGCCTTTGCTGCTGAAGCCCCTGGGGTC GCCTCTACCTGATGAGGATGTGCACCCCCATTAGGGGGCTGCTCATGGCCCTTGCAGTGA TGTTTGGGACAGCGATGGCATTTGCACCCATACCCCGGATCACCTGGGAGCACAGAGAGG TGCACCTGGTGCAGTTTCATGAGCCAGACATCTACAACTACTCAGCCTTGCTGCTGAGCG AGGACAAGGACACCTTGTACATAGGTGCCCGGGAGGCGGTCTTCGCTGTGAACGCACTCA ACATCTCCGAGAAGCAGCATGAGGTGTATTGGAAGGTCTCAGAAGACAAAAAAGCAAAAT GTGCAGAAAAGGGGAAATCAAAACAGACAGAGTGCCTCAACTACATCCGGGTGCTGCAGC CACTCAGCGCCACTTCCCTTTACGTGTGTGGGACCAACGCATTCCAGCCGGCCTGTGACC ACCTGAACTTAACATCCTTTAAGTTTCTGGGGAAAAATGAAGATGGCAAAGGAAGATGTC CCTTTGACCCAGCACACAGCTACACATCCGTCATGGTTGATGGAGAACTTTATTCGGGGA CGTCGTATAATTTTTTGGGAAGTGAACCCATCATCTCCCGAAATTCTTCCCACAGTCCTC TGAGGACAGAATATGCAATCCCTTGGCTGAACGAGCCTAGTTTCGTGTTTGCTGACGTGA TCCGAAAAAGCCANCAGCCCCGCGGCGAGATGACAGGGTCTACTTTTCTACGGAGGTGTT GTGAGATGAATTTGTGGTCCAAGGGGGCTGATCCCCCGATGCCAGAGTGGCAAGGGGGAC CCAGGCGGCCTGAGGACCTTGCAAAAGAATGAACCTCTTCCT >OriGene 3' read for NM_006378 unedited
GCTGTGGCACAAAACCGCGCTCGANAGTCGAGTTATTTCTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTAAAAGCTTCTCATTTATTTGGGTACTTTCCAAATGTTTACAATTCAAAGTA AAAAAATAAAAACAAGGTAAATCTTATAAAACACAAATGTTTACCCCAAAATGGTCAAAT CCTATCATGCTGGAAAATAAAACTGTCCACACACTTCTTCTTACATCCAAAGTTTATTGC TCTTCACCATGGAAAAAAAGTCTTTTCCCGATGGGTATTTACAAAGCGGGGAAATGGAGT GTGAAATTAACCAGTGTTGAAATCGTACCACCCATTCACATTTTCAGGACAGTGGCAGGA ACAGCGCCGTATGCTTGAGGGTGCTGTGGTCACAGCTCGCGGCCCCGGCCACCGAGGCCC AGTTCCCTTCCAGTAGTGGGCAGCTCAGTGAGGCTGGATAAAAAGTCTGGGCAGGCAGTG GGTAACCAATTGAAGCAAGAAAAAAAAGGAGATGTTCAAAAAACCAAAGGCGCCCTTGTG AAGGTTCCATCTGTCTCACAGGAGGTTCTGACCGACAGCTGTGGGCTCCAAATTGTACCG CACTCCCATTTCAGCTCTGGGCCAGCCTGGCTCCACTCAGCTTATCCAGGCTACCTTTCC TTGCTCAAAACCCACCTCGTGGATGTGGGAAGGCCCTCTTCTTTCAAAGAGTAAAAGTTT AAAAAAAAGAAAAGTAAACCAACTCCAAAAAGAAAATTTTTTCAAAAACAATGCTTCCCT CAAGGAATAAAGGTCAAAAAAAAAAAAAAAAAAGAAAAAAGGGGAATGGGCCCCCGGGGA AGTGTTTATTCTCCTTTCTTAAAACCAAAAGATTGCCTTTACAAAAGAAATTTTTTCTCT GAAATTCTTACCACCGAGCCCCGTTTTTCTCCCATAATTAACCAAAATTGGGAATAATCA CCTTTTTTAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_006378 |
| Insert Size | 5030 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006378.2, NP_006369.2 |
| RefSeq Size | 4675 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 10507 |
| UniProt ID | Q92854 |
| Domains | Sema, PSI, ig, IG, PSI |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Axon guidance |
| Gene Summary | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed:20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed:19788569). Promotes the migration of cerebellar granule cells (PubMed:16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed:8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed:16055703).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulated surface expression and shedding support a dual role for semaphorin 4D in platelet responses to vascular injury
,Li Zhu, Wolfgang Bergmeier, Jie Wu, Hong Jiang, Timothy J Stalker, Marcin Cieslak, Ran Fan, Laurence Boumsell, Atsushi Kumanogoh, Hitoshi Kikutani, Luca Tamagnone, Denisa D Wagner, Marcos E Milla, and Lawrence F Brass,
Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1621-6.
[Sema4D]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208521 | SEMA4D (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1 |
CNY 9,472.00 |
|
| RC208521L1 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1, Myc-DDK-tagged |
CNY 11,872.00 |
|
| RC208521L2 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208521L3 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1, Myc-DDK-tagged |
CNY 11,872.00 |
|
| RC208521L4 | Lenti ORF clone of Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1, mGFP tagged |
CNY 11,872.00 |
|
| RG208521 | SEMA4D (tGFP-tagged) - Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1 |
CNY 11,072.00 |
|
| SC323843 | SEMA4D (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), transcript variant 1 |
CNY 9,480.00 |
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