Hsc70 (HSPA8) (NM_006597) Human Untagged Clone

Name: Hsc70 (HSPA8) (NM_006597) Human Untagged Clone
SKU: SC115973
Price: 4816.00

CAT＃: SC115973

HSPA8 (untagged)-Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1

Datasheet

SDS

"NM_006597" in other vectors (7)

CNY 2,950.00

货期*

现货

规格

Cited in 4 publications.

Product images

经常一起买 (4)

TA505966

HSPA8 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC115973 sequence for NM_006597 edited (data generated by NextGen Sequencing) ATGTCCAAGGGACCTGCAGTTGGTATTGATCTTGGCACCACCTACTCTTGTGTGGGTGTT TTCCAGCACGGAAAAGTCGAGATAATTGCCAATGATCAGGGAAACCGAACCACTCCAAGC TATGTCGCCTTTACGGACACTGAACGGTTGATCGGTGATGCCGCAAAGAATCAAGTTGCA ATGAACCCCACCAACACAGTTTTTGATGCCAAACGTCTGATTGGACGCAGATTTGATGAT GCTGTTGTCCAGTCTGATATGAAACATTGGCCCTTTATGGTGGTGAATGATGCTGGCAGG CCCAAGGTCCAAGTAGAATACAAGGGAGAGACCAAAAGCTTCTATCCAGAGGAGGTGTCT TCTATGGTTCTGACAAAGATGAAGGAAATTGCAGAAGCCTACCTTGGGAAGACTGTTACC AATGCTGTGGTCACAGTGCCAGCTTACTTTAATGACTCTCAGCGTCAGGCTACCAAAGAT GCTGGAACTATTGCTGGTCTCAATGTACTTAGAATTATTAATGAGCCAACTGCTGCTGCT ATTGCTTACGGCTTAGACAAAAAGGTTGGAGCAGAAAGAAACGTGCTCATCTTTGACCTG GGAGGTGGCACTTTTGATGTGTCAATCCTCACTATTGAGGATGGAATCTTTGAGGTCAAG TCTACAGCTGGAGACACCCACTTGGGTGGAGAAGATTTTGACAACCGAATGGTCAACCAT TTTATTGCTGAGTTTAAGCGCAAGCATAAGAAGGACATCAGTGAGAACAAGAGAGCTGTA AGACGCCTCCGTACTGCTTGTGAACGTGCTAAGCGTACCCTCTCTTCCAGCACCCAGGCC AGTATTGAGATCGATTCTCTCTATGAAGGAATCGACTTCTATACCTCCATTACCCGTGCC CGATTTGAAGAACTGAATGCTGACCTGTTCCGTGGCACCCTGGACCCAGTAGAGAAAGCC CTTCGAGATGCCAAACTAGACAAGTCACAGATTCATGATATTGTCCTGGTTGGTGGTTCT ACTCGTATCCCCAAGATTCAGAAGCTTCTCCAAGACTTCTTCAATGGAAAAGAACTGAAT AAGAGCATCAACCCTGATGAAGCTGTTGCTTATGGTGCAGCTGTCCAGGCAGCCATCTTG TCTGGAGACAAGTCTGAGAATGTTCAAGATTTGCTGCTCTTGGATGTCACTCCTCTTTCC CTTGGTATTGAAACTGCTGGTGGAGTCATGACTGTCCTCATCAAGCGTAATACCACCATT CCTACCAAGCAGACACAGACCTTCACTACCTATTCTGACAACCAGCCTGGTGTGCTTATT CAGGTTTATGAAGGCGAGCGTGCCATGACAAAGGATAACAACCTGCTTGGCAAGTTTGAA CTCACAGGCATACCTCCTGCACCCCGAGGTGTTCCTCAGATTGAAGTCACTTTTGACATT GATGCCAATGGTATACTCAATGTCTCTGCTGTGGACAAGAGTACGGGAAAAGAGAACAAG ATTACTATCACTAATGACAAGGGCCGTTTGAGCAAGGAAGACATTGAACGTATGGTCCAG GAAGCTGAGAAGTACAAAGCTGAAGATGAGAAGCAGAGGGACAAGGTGTCATCCAAGAAT TCACTTGAGTCCTATGCCTTCAACATGAAAGCAACTGTTGAAGATGAGAAACTTCAAGGC AAGATTAACGATGAGGACAAACAGAAGATTCTGGACAAGTGTAATGAAATTATCAACTGG CTTGATAAGAATCAGACTGCCGAGAAGGAAGAATTTGAACATCAACAGAAAGAGCTGGAG AAAGTTTGCAACCCCATCATCACCAAGCTGTACCAGAGTGCAGGAGGCATGCCAGGAGGA ATGCCTGGGGGATTTCCTGGTGGTGGAGCTCCTCCCTCTGGTGGTGCTTCCTCAGGGCCC ACCATTGAAGAGGTTGATTAA Clone variation with respect to NM_006597.4 1761 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_006597 unedited GGTTACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCTCGCCTGCA GCTCTTGGGTTTTTTGTGGCTTCCTTCGTTATTGGAGCCAGGCCTACATCCCAGCAACCA TGTCCAAGGGACCTGCAGTTGGTATTGATCTTGGCACCACCTACTCTTGTGTGGGTGTTT TCCAGCACGGTAAAAGTCGAGATAATTGCCAATGATCAGGGAAACCGAACCACTCCAAGC TATGTCGCCTTTACGGACACTGAACGGTTGATCGGTGATGCCGCAAAGAATCAAGTTGCA ATGAACCCCACCAACACAGTTTTTGATGCCAAACGTCTGATTGGACGCAGATTTGATGAT GCTGTTGTCCAGTCTGATATGAAACATTGGCCCTTTATGGTGGTGAATGATGCTGGCAGG CCCAAGGTCCAAGTAGAATACAAGGGAGAGACCAAAAGCTTCTATCCAGAGGAGGTGTCT TCTATGGTTCTGACAAAGATGAAGGAAATTGCAGAAGCCTACCTTGGGAAGACTGTTACC AATGCTGTGGTCACAGTGCCAGCTTACTTTAATGACTCTCAGCGTCAGGCTACCAAAGAT GCTGGAACTATTGCTGGTCTCAATGTACTTAGAATTATTAATGAGCCAACTGCTGCTGCT ATTGCTTACGGCTTAGACAAAAAGGTTGGAGCAGAAAGAAACGTGCTCATCTTTGACCTG GGAGGTGGCACTTTTGATGTGTCAATCCTCACTATTNGAGATGGAATCTTTGAGGTCAAG TCTACAGCTGGAGACACCCACTTGGGTGGAGAAGATTNTGACAACCGAATGGGTCACCAT TTTATTGCTGAGTTTAAGCGCAAGCTTAGAAGGACATCAGTGAGAACAAGAGAGCTGTNA GAACGCTCCGTACTGCCTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_006597 unedited ATGGCAACTTCCAGGGCCAGNATAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGGAT CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT TTTTGCCAATTTTAAATAGTTTTATTTAAGACATTGCATTTTCCACTTACAATACAGTGT TTATAAAGTGCAATGTTATTTCCTTCCCCTGTGCATATGTTCCATATTCAAGTATTGAGA ATGCCCAGTAACTTACTATAGCAGCTTAACTTTTTAAAACTGCCACAGAATTTGCTACGA ATTTAGGTCCTTCAAATGTTTTAAATGTGTGGAACAATGCTACATCTACACTTGGTTGGC TTAATCAACCTCTTCAATGGTGGGCCCTGAGGAAGCACCACCAGAGGGAGGAGCTCCACC ACCAGGAAATCCCCCAGGCATTCCTCCTGGCATGCCTCCTGCACTCTGGTACAGCTTGGT GATGATGGGGTTGCAAACTTTCTCCAGCTCTTTCTGTTGATGTTCAAATTCTTCCTTCTC GGCAGTCTGATTCTTATCAAGCCAGTTGATAATTTCATTACACTTGTCCAGAATCTTCTG TTTGTCCTCATCGTTAATCTTGCCTTGAAGTTTCTCATCTTCAACAGTTGCTTTCATGTT GAAGGCATAGGACTCAAGTGAATTCTTGGATGACANCCTTGTCCCTCTGCTTCTCATCTT CAGCTTTGTACTTCTCAGCTTCCTGGACCATACGTTCAATGGTCTTCTTGCTCAAACGNC CCTTGNATTAGNGATAGTAATCTTGGTCTCTTTTCCCCGTACTCTTNGCCACAGCAGAGA CATTGAGGATACCATTGGCATCATGGCAAAAGTGACTTCATCCTGAGACACC
Restriction Sites	NotI-NotI
ACCN	NM_006597
Insert Size	2190 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_006597.3, NP_006588.1
RefSeq Size	2276 bp
RefSeq ORF	1941 bp
Locus ID	3312
UniProt ID	P11142
Domains	HSP70
Protein Families	Stem cell - Pluripotency
Protein Pathways	Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome
Gene Summary	This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (4)

The use of this cDNA Clones has been cited in the following citations:
Hsc70 facilitates TGF-ß-induced activation of Smad2/3 in fibroblastic NRK-49F cells ,Ikezaki, M;Higashimoto, N;Matsumura, K;Ihara, Y;, Biochem. Biophys. Res. Commun. ,PubMed ID 27320859 [HSPA8]
Isoform Selective Genetic Inhibition of Constitutive Cytosolic Hsp70 Activity Promotes Client Tau Degradation Using an Altered Co-chaperone Complement ,Stothert, UK;Sabbagh, JJ;Chang, L;Stevens, SM;Jr, E;, JOURNAL OF BIOLOGICAL CHEMISTRY ,PubMed ID 25864199 [HSPA8]
The active Hsc70/tau complex can be exploited to enhance tau turnover without damaging microtubule dynamics ,Fontaine, SN;Martin, MD;Akoury, E;Assimon, VA;Borysov, S;Nordhues, BA;Sabbagh, JJ;Cockman, M;Gestwicki, JE;Zweckstetter, M;Dickey, CA;, Hum. Mol. Genet. ,PubMed ID 25882706 [HSPA8]
C-Mannosylated peptides derived from the thrombospondin type 1 repeat interact with Hsc70 to modulate its signaling in RAW264.7 cells ,Yoshito Ihara, Shino Manabe, Midori Ikezaki, Yoko Inai, In-Sook Lee Matsui, Yuriko Ohta, Eiji Muroi, and Yukishige Ito, Glycobiology, Oct 2010; 20: 1298 - 1310 [HSPA8]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC202209	HSPA8 (Myc-DDK-tagged)-Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,560.00
RC202209L1	Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC202209L2	Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC202209L3	Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,208.00
RC202209L4	Lenti ORF clone of Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RG202209	HSPA8 (tGFP-tagged) - Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,040.00
SC322471	HSPA8 (untagged)-Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,950.00

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