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MGAT4A (NM_012214) Human Untagged Clone
CAT＃: SC115491
MGAT4A (untagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1
CNY 5,984.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GnT-4a; GNT-IV; GNT-IVA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115491 sequence for NM_012214 edited (data generated by NextGen Sequencing)
ATGAGGCTCCGCAATGGAACTGTAGCCACTGCTTTAGCATTTATCACTTCCTTCCTTACT TTGTCTTGGTATACTACATGGCAAAATGGGAAAGAAAAACTGATTGCTTATCAACGAGAA TTCCTTGCTTTGAAAGAACGTCTTCGAATAGCTGAACACAGAATCTCACAGCGCTCTTCT GAATTAAATACGATTGTGCAACAGTTCAAGCGTGTAGGAGCAGAAACAAATGGAAGTAAG GATGCGTTGAATAAGTTTTCAGATAATACCCTAAAGCTGTTAAAGGAGTTAACAAGCAAA AAATCTCTTCAAGTGCCAAGTATTTATTATCATTTGCCTCATTTATTGAAAAATGAAGGA AGTCTTCAACCTGCTGTACAGATTGGCAACGGAAGAACAGGAGTTTCAATAGTCATGGGC ATTCCCACAGTGAAGAGAGAAGTTAAATCTTACCTCATAGAAACTCTTCATTCCCTTATT GATAACCTGTATCCTGAAGAGAAGTTGGACTGTGTTATAGTAGTCTTCATAGGAGAGACA GATATTGATTATGTACATGGTGTTGTAGCCAACCTGGAGAAAGAATTTTCTAAAGAAATC AGTTCTGGCTTGGTGGAAGTCATATCACCCCCTGAAAGCTATTATCCTGACTTGACAAAC CTAAAGGAGACATTTGGAGACTCCAAAGAAAGAGTAAGATGGAGAACAAAGCAAAACCTA GATTACTGTTTTCTAATGATGTATGCTCAAGAAAAGGGCATATATTACATTCAGCTTGAA GATGATATTATTGTCAAACAAAATTATTTTAATACCATAAAAAATTTTGCACTTCAACTT TCTTCTGAGGAATGGATGATTCTAGAGTTTTCCCAGCTGGGCTTCATTGGTAAAATGTTT CAAGCGCCGGATCTTACTCTGATTGTAGAATTCATATTCATGTTTTACAAGGAGAAACCC ATTGATTGGCTCCTGGACCATATTCTCTGGGTGAAAGTCTGCAACCCTGAAAAAGATGCA AAACATTGTGATAGACAGAAAGCAAATCTGCGAATTCGCTTCAGACCTTCCCTTTTCCAA CATGTTGGTCTGCACTCATCACTATCAGGAAAAATCCAAAAACTCACGGATAAAGATTAT ATGAAACCATTACTTCTTAAAATCCATGTAAACCCACCTGCGGAGGTATCTACTTCCTTG AAGGTCTACCAAGGGCATACGCTGGAGAAAACTTACATGGGAGAGGATTTCTTCTGGGCT ATCACACCGATAGCTGGAGACTACATCTTGTTTAAATTTGATAAACCAGTCAATGTAGAA AGTTATTTGTTCCATAGCGGCAACCAAGAACATCCTGGAGATATTCTGCTAAACACAACT GTGGAAGTTTTGCCTTTTAAGAGTGAAGGTTTGGAAATAAGCAAAGAAACCAAAGACAAA CGATTAGAAGATGGCTATTTCAGAATAGGAAAATTTGAGAATGGTGTTGCAGAAGGAATG GTGGATCCAAGTCTCAATCCCATTTCAGCCTTTCGACTTTCAGTTATTCAGAATTCTGCT GTTTGGGCCATTCTTAATGAGATTCATATTAAAAAAGCCACCAACTGA Clone variation with respect to NM_012214.2 >OriGene 5' read for NM_012214 unedited
GGGGTCAGGAACNGAAAACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGCCAGCGG CGGGAAGGGAAAAGGCCGAGGCATCAGCGTGTGAAGACCGCAAAGACGATCCCGAGTACA GTTGTGAACAGCATTGCTGCTAGGCTCCTCCTGCAGATCATCTGAAATGAACCTCTCTTA TTGATTTTTATTGGCCTAGAGCCAGGAGTACTGCATTCAGTTGACTTTCAGGGTAAAAAG AAAACAGTCCTGGTTGTTGTCATCATAAACATATGGACCAGTGTGATGGTGAAATGAGAT GAGGCTCCGCAATGGAACTGTAGCCACTGCTTTAGCATTTATCACTTCCTTCCTTACTTT GTCTTGGTATACTACATGGCAAAATGGGAAAGAAAAACTGATTGCTTATCAACGAGAATT CCTTGCTTTGAAAGAACGTCTTCGAATAGCTGAACACAGAATCTCACAGCGCTCTTCTGA ATTAAATACGATTGTGCAACAGTTCAAGCGTGTAGGAGCAGAAACAAATGGAAGTAAGGA TGCGTTGAATAAGTTTTCAGATAATACCCTAAAGCTGTTAAAGGAGTTAACAAGCAAAAA ATCTCTTCAAGTGCCAAGTATTTATTATCATTTGCCTCATTTATTGAAAAATGAAGGAAG TCTTCAACCTGCTGTACAGATTGGCAACGGAAGAACAGGAGTTTCAATAGTCATGGGCAT TCCCACAGTGAAGAGAGAAGTTAAATCTTACCTCATAGAAACTCTTCATTCCCTTATTTG ATACCTGTATCCTGAAGAGAAGTTGGACTGTGTTATAGTAGTCTTCATAGGAGAGACAGA TATTGATTATGTACATGGNTGTCGTAGCCCACCTGGAGAAAGAATTNNTCTAAGNAANAT CAGTCTGGCGGGNNGGGAGTCATATCACCA >OriGene 3' read for NM_012214 unedited
GGGGGCACCCCNAGCNCCTTCTANNAAACNGGTAATCTCTGNACCGCGGNCCGCATNCTA GGATCGAGNNNTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGGC TAAACTATTTTTAATTAACAAATTCACAGGAAAAAAAGGGGTTGGTTTTCTACAAATGAT CCAGTGGGGGGCTTTTTTTAATATGAATCTCATTAAAAAGGGCCCAAACAGCAAAATTTT GAATAACTGAAAGTCAAAGGGCTGAAAGGGGATTGAAACTTGAATCCACCTTTCTTTTTG CAACACCTTTCTAAAATTTTCCTATTCTGAAATACCCATCTTCTAACCGTTGGCCTTTGG TTTCTTTGCTTATTCCCAAACCTTCATTTTTAAAAGGCAAAACTTCCACAGTTGTGTTTA CCAAAATATCTCCAGAATGTTCTTGGTTGCCGTTATGGAACAAATAACTTTCTACATTGA CGGGTTTATCAAATTTAACCAAGATGTACTCTCCAGCTATCGGTGTGATAGCCCAGAAGA AATCCTCTCCATGTAAGTTTTCCCCAGCGTTTGCCCTTGGTAAACCTTCAAGGAAGAAAA TACTTCCCCAGGGTGGTTTACCTGGATTTAAAGAANAATGGGTTAATATAATCTTTATCC CGGAGTTTTTGGATTTTTCCTGAAGGGAAGAGGGCCAACCAACTGTTGAAAGGGGAGGTT TGAACCAAATTCCAAATTGCTTTTTGCTTATCCATGTTTGGCTTTTTTTAGGGTTGGCAA CTTTCCCCCAAAAAATGGGCCCAGACCCAACAAGGGGTTTTCCCTGGAAAAAGGAATTGA ATTTTCCCCCCCGGAAAAAACCCGGGGTTGGAAACATTTTCCCATGAACCCCCCTTGGGA AACCTTTAAATTTTCCCTTTCTTCCAAAAAGGGGGAGGGCCAAAATTTTTATGGGATAAA AAAATTTTTGTTGAAAAAAATTCCTCTTCCACCGGAGGGGAAATGGCCTTTTTTTGGCAC AACTATTAAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012214 |
| Insert Size | 1960 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012214.1, NP_036346.1 |
| RefSeq Size | 2115 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 11320 |
| UniProt ID | Q9UM21 |
| Domains | Glyco_transf_55 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213802 | MGAT4A (Myc-DDK-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1 |
CNY 5,976.00 |
|
| RC213802L1 | Lenti-ORF clone of MGAT4A (Myc-DDK-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1 |
CNY 8,376.00 |
|
| RC213802L2 | Lenti-ORF clone of MGAT4A (mGFP-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1 |
CNY 6,460.00 |
|
| RC213802L3 | Lenti-ORF clone of MGAT4A (Myc-DDK-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1 |
CNY 6,460.00 |
|
| RC213802L4 | Lenti-ORF clone of MGAT4A (mGFP-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1 |
CNY 6,460.00 |
|
| RG213802 | MGAT4A (tGFP-tagged) - Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1 |
CNY 7,576.00 |
