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Translation factor GUF1, mitochondrial (GUF1) (NM_021927) Human Untagged Clone
CAT＃: SC112878
GUF1 (untagged)-Human GUF1 GTPase homolog (S. cerevisiae) (GUF1)
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEE40; EF-4; EF4; EIEE40 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_021927, the custom clone sequence may differ by one or more nucleotides
ATGTGGACCCTCGTGGGTCGGGGCTGGGGGTGCGCACGCGCTCTCGCGCCACGAGCCACTGGGGCCGCGC TTCTGGTGGCCCCGGGGCCCCGGTCCGCGCCGACCCTTGGGGCTGCTCCAGAGTCCTGGGCTACCGACAG GCTCTACAGCTCCGCAGAATTCAAGGAAAAACTTGACATGTCTAGGTTTCCTGTTGAAAATATTAGAAAT TTCAGTATTGTTGCACACGTGGATCATGGCAAAAGTACTTTAGCTGACAGGCTCCTAGAACTTACAGGGA CAATTGATAAAACAAAGAATAATAAGCAGGTTCTTGATAAATTGCAAGTGGAACGAGAAAGAGGAATCAC TGTTAAAGCACAGACAGCATCTCTCTTTTACAATTGTGAAGGAAAGCAGTACCTTTTAAATCTCATTGAT ACACCGGGCCATGTTGATTTTAGTTATGAAGTATCCAGGTCACTTTCTGCTTGCCAGGGTGTTTTACTTG TGGTTGATGCAAATGAGGGAATTCAAGCCCAAACTGTAGCAAACTTCTTTCTTGCCTTCGAAGCACAGCT ATCGGTAATTCCAGTTATAAATAAGATAGATCTGAAGAATGCTGATCCTGAAAGGGTTGAAAACCAAATT GAGAAAGTGTTTGATATTCCAAGTGATGAATGTATTAAGATTTCTGCTAAACTTGGAACAAATGTTGAGA GTGTTCTTCAGGCAATTATTGAAAGAATCCCCCCTCCTAAAGTGCATCGCAAAAATCCTCTGAGAGCTTT GGTATTTGACTCCACCTTTGACCAGTATAGAGGTGTGATAGCCAATGTAGCATTATTTGACGGAGTGGTT TCCAAAGGAGATAAAATTGTATCTGCACATACTCAAAAGACATACGAAGTTAATGAAGTAGGAGTCTTGA ATCCTAATGAGCAGCCAACTCATAAATTATATGCAGGACAGGTGGGCTATCTGATTGCTGGGATGAAAGA TGTCACTGAAGCGCAAATAGGAGATACATTATGTTTACATAAGCAACCAGTGGAGCCCTTGCCTGGGTTT AAATCAGCGAAACCAATGGTATTTGCAGGAATGTATCCTCTAGACCAATCTGAATATAACAATCTGAAGA GTGCTATAGAAAAACTGACTTTAAATGATTCCAGTGTGACCGTTCATCGGGATAGTAGCCTTGCTCTGGG TGCTGGCTGGAGGCTAGGATTTCTTGGACTTTTGCACATGGAAGTTTTCAACCAGCGACTGGAGCAAGAA TATAATGCTTCTGTTATTTTAACAACCCCTACTGTTCCATATAAAGCTGTACTGTCATCATCAAAATTGA TAAAGGAACATAGAGAAAAAGAAATTACAATTATCAATCCTGCACAATTCCCCGATAAATCAAAAGTAAC AGAATATTTGGAGCCAGTTGTTTTGGGCACTATTATCACACCAGATGAATACACTGGAAAAATAATGATG CTTTGCGAGGCTCGAAGAGCAGTTCAGAAGAATATGATATTTATTGATCAAAATAGAGTTATGCTTAAAT ATCTCTTTCCTTTGAATGAAATTGTGGTAGATTTTTATGACTCTTTGAAATCCCTATCTTCTGGATATGC TAGTTTTGATTACGAAGATGCAGGCTACCAGACTGCAGAACTTGTAAAAATGGATATTCTACTGAATGGA AATACTGTAGAGGAGCTAGTAACTGTTGTACACAAAGACAAAGCTCATTCAATTGGCAAAGCCATATGTG AACGGCTGAAGGATTCTCTTCCTAGGCAACTGTTTGAGATAGCAATTCAAGCTGCTATTGGAAGTAAAAT CATTGCAAGAGAAACTGTGAAAGCCTATAGGAAAAACGTTTTGGCAAAATGTTATGGTGGTGATATTACC CGAAAAATGAAGCTTTTGAAGAGACAAGCAGAAGGGAAAAAAAAGCTGAGGAAAATTGGCAACGTTGAAG TTCCAAAAGATGCTTTTATAAAAGTTCTGAAAACACAATCTTCTAAATAA >OriGene 5' read for NM_021927 unedited
TTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGCCGGATCTGGTACT TGGGCAGAGCTCCCCGGGGTTCATTGTCTTCGCTTCACAGGATCTGTTTGAGTCCTGTCC ACCGGATCCTACGGGGGGTACCTTCGAAAAAAAACGGGCTATGCTGCTGTTGCGTGTGGG TACCCTCTCCTGACGCCTCCGCCGCCCGGGTCATGTGGACCCTCGTGGGTCGGGGCTGGG GGTGCGCACGCGCTCTCGCGCCACGAGCCACTGGGGCCGCGCTTCTGGTGGCCCCGGGGC CCCGGTCCGCGCCGACCCTTGGGGCTGCTCCAGAGTCCTGGGCTACCGACAGGCTCTACA GCTCCGCAGAATTCAAGGAAAAACCTGACATGTCTAGGTTTCCTGTTGAAAATATTAGAA ATTTCAGTATTGTTGCACACGTGGATCATGGCAAAAGTACTTTAGCTGACAGGCTCCTAG AACTTACAGGGACAATTGATAAAACAAAGAATAATAAGCAGGTTCTTGATAAATTGCAAG TGGAACGAGAAAGAGGAATCACTGTTAAAGCACAGACAGCATCTCTCTTTTACAATTGTG AAGGAAAGCAGTACCTTTTAAATCTCATTGATACACCGGGCCATGTTGATTNTAGTTATG AAGTATCCAGGTCACTTTCTGCTTGCCAGGGTGTTTTACTTGTGGTTGATGCANATGAGG GAATTCAAGCCCAACTGTAGCAAACTTCTTTCTTGCCTTCGAAGCACAGCTATCGGTAAT TCCAGTTATAAATAAGATAGATCCTGAGAATGCTGATCCTGAAAAGTTTGAAAACCAATT GAGAAAGTGTTTGATATCCAAGTGATGAATGTTTAAGAATTCTGCTTAACTTGGACAAAT GTTGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021927 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021927.1, NP_068746.1 |
| RefSeq Size | 2671 bp |
| RefSeq ORF | 2010 bp |
| Locus ID | 60558 |
| UniProt ID | Q8N442 |
| Domains | EFG_C, GTP_EFTU, GTP_EFTU_D2 |
| Gene Summary | This gene encodes a GTPase that triggers back-translocation of the elongating ribosome during mitochondrial protein synthesis. The protein contains a highly conserved C-terminal domain not found in other GTPases that facilitates tRNA binding. The encoded protein is thought to prevent misincorporation of amino acids in stressful, suboptimal conditions. An allelic variant in this gene has been associated with early infantile epileptic encephalopathy-40. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207735 | GUF1 (Myc-DDK-tagged)-Human GUF1 GTPase homolog (S. cerevisiae) (GUF1) |
CNY 7,352.00 |
|
| RC207735L3 | Lenti ORF clone of Human GUF1 GTPase homolog (S. cerevisiae) (GUF1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207735L4 | Lenti ORF clone of Human GUF1 GTPase homolog (S. cerevisiae) (GUF1), mGFP tagged |
CNY 7,700.00 |
|
| RG207735 | GUF1 (tGFP-tagged) - Human GUF1 GTPase homolog (S. cerevisiae) (GUF1) |
CNY 8,952.00 |
