ASIC3 (NM_020321) Human Untagged Clone
CAT#: SC110043
ASIC3 (untagged)-Human amiloride-sensitive cation channel 3 (ACCN3), transcript variant 2
CNY 4,096.00
CNY 8,840.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ACCN3; DRASIC; SLNAC1; TNaC1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC110043 sequence for NM_020321 edited (data generated by NextGen Sequencing)
ATGAAGCCCACCTCAGGCCCAGAGGAGGCCCGGCGGCCAGCCTCGGACATCCGCGTGTTC GCCAGCAACTGCTCGATGCACGGGCTGGGCCACGTCTTCGGGCCAGGCAGCCTGAGCCTG CGCCGGGGGATGTGGGCAGCGGCCGTGGTCCTGTCAGTGGCCACCTTCCTCTACCAGGTG GCTGAGAGGGTGCGCTACTACAGGGAGTTCCACCACCAGACTGCCCTGGATGAGCGAGAA AGCCACCGGCTCATCTTCCCGGCTGTCACCCTGTGCAACATCAACCCACTGCGCCGCTCG CGCCTAACGCCCAACGACCTGCACTGGGCTGGGTCTGCGCTGCTGGGCCTGGATCCCGCA GAGCACGCCGCCTTCCTGCGCGCCCTGGGCCGGCCCCCTGCACCGCCCGGCTTCATGCCC AGTCCCACCTTTGACATGGCGCAACTCTATGCCCGTGCTGGGCACTCCCTGGATGACATG CTGCTGGACTGTCGCTTCCGTGGCCAACCTTGTGGGCCTGAGAACTTCACCACGATCTTC ACCCGGATGGGAAAGTGCTACACATTTAACTCTGGCGCTGATGGGGCAGAGCTGCTCACC ACTACTAGGGGTGGCATGGGCAATGGGCTGGACATCATGCTGGACGTGCAGCAGGAGGAA TATCTACCTGTGTGGAGGGACAATGAGGAGACCCCGTTTGAGGTGGGGATCCGAGTGCAG ATCCACAGCCAGGAGGAGCCGCCCATCATCGATCAGCTGGGCTTGGGGGTGTCCCCGGGC TACCAGACCTTTGTTTCTTGCCAGCAGCAGCAGCTGAGCTTCCTGCCACCGCCCTGGGGC GATTGCAGTTCAGCATCTCTGAACCCCAACTATGAGCCAGAGCCCTCTGATCCCCTAGGC TCCCCCAGCCCCAGCCCCAGCCCTCCCTATACCCTTATGGGGTGTCGCCTGGCCTGCGAA ACCCGCTACGTGGCTCGGAAGTGCGGCTGCCGAATGGTGTACATGCCAGGCGACGTGCCA GTGTGCAGCCCCCAGCAGTACAAGAACTGTGCCCACCCGGCCATAGATGCCATGCTTCGC AAGGACTCGTGCGCCTGCCCCAACCCGTGCGCCAGCACGCGCTACGCCAAGGAGCTCTCC ATGGTGCGGATCCCGAGCCGCGCCGCCGCGCGCTTCCTGGCCCGGAAGCTCAACCGCAGC GAGGCCTACATCGCGGAGAACGTGCTGGCCCTGGACATCTTCTTTGAGGCCCTCAACTAT GAGACCGTGGAGCAGAAGAAGGCCTATGAGATGTCAGAGCTGCTTGGTGACATTGGGGGC CAGATGGGGCTGTTCATCGGGGCCAGCCTGCTCACCATCCTCGAGATCCTAGACTACCTC TGTGAGGTGTTCCGAGACAAGGTCCTGGGATATTTCTGGAACCGACAGCACTCCCAAAGG CACTCCAGCACCAATCTGCTTCAGGAAGGGCTGGGCAGCCATCGAACCCAAGTTCCCCAC CTCAGCCTGGGCCCCAGCACTCTGCTCTGTTCCGAAGACCTCCCACCCCTCCCTGTGCCG TCACCAAGACTCTCTCCGCCTCCCACCGCACCTGCTACCTTGTCACACAGCTCTAGACCT GCTGTCTGTGTCCTCGGAGCCCCGCCCTGA Clone variation with respect to NM_020321.2 >OriGene 5' read for NM_020321 unedited
NCGGTTAGATTTTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACCAGCTGCCTTC CAACCTTGGCTGTCTCCCACCCTCTCTTCTCCTCTCCTTGCCTGGCCTCCTGAATCCTAT CTTAGCCTCCTTAGCCCCCTGACTGACTCTCTCTCGCTTCTTCCAAGCCTCTGTAGCTGG TTCCGCTCCTGGGTTCTGGCCATGAAGCCCACCTCAGGCCCAGAGGAGGCCCGGCGGCCA GCCTCGGACATCCGCGTGTTCGCCAGCAACTGCTCGATGCACGGGCTGGGCCACGTCTTC GGGCCAGGCAGCCTGAGCCTGCGCCGGGGGATGTGGGCAGCGGCCGTGGTCCTGTCAGTG GCCACCTTCCTCTACCAGGTGGCTGAGAGGGTGCGCTACTACAGGGAGTTCCACCACCAG ACTGCCCTGGATGAGCGAGAAAGCCACCGGCTCATCTTCCCGGCTGTCACCCTGTGCAAC ATCAACCCACTGCGCCGCTCGCGCCTAACGCCCAACGACCTGCACTGGGCTGGGTCTGCG CTGCTGGGCCTGGATCCCGCAGAGCACGCCGCCTTCCTGCGCGCCCTGGGCCGGCCCCCT GCACCGCCCGGCTTCATGCCCAGTCCCACCTTTGACATGGCGCAACTCTATGCCCGTGCT GGGCACTCCCTGGATGACATGCTGCTGGACTGTCGCTTCCGTGGCCAACCCTTGTGGGCC TGAGAACTTCACCACGATCTTCACCCGGATGGGAAAGTGCTACACATTTAACTCTGGCGC TGATGGGGCAGAGCTGCTCACCACTACTAGGGGTGGCATGGGGCAATGGGCTGAACATCA TGCTNGACGTGCAGCAGGAGGAATATCTACCTGTGTGGAGGGACAAATGAGAGACCCCGT TTGAGGTGGGGATCCGATTGCAGATCCCCANCCAGG >OriGene 3' read for NM_020321 unedited
TTTTGCCGTACNTAGTACCGCGCCGCTTTCTANGATCNGTTTTTTTTTTTTTTTTTTTGC TGATGCATTAGGACTTTATTTGGGGTGAAGACGGAAAAGCTACGTGCAGGCTAGGCATGT CCAGGATGTCAGGGCGGGGCTCCGAGGACACAGACAGCAGGTCTAGAGCTGTGTGACAAG GTAGCAGGTGCGGTGGGAGGCGGAGAGAGTCTTGGTGACGGCACAGGGAGGGGTGGGAGG TCTTCGGAACAGAGCAGAGTGCTGGGGCCCAGGCTGAGGTGGGGAACTTGGGTTCGATGG CTGCCCAGCCCTTCCTGAAGCAGATTGGTGCTGGAGTGCCTTTGGGAGTGCTGTCGGTTC CAGAAATATCCCAGGACCTTGTCTCGGAACACCTCACAGAGGTAGTCTAGGATCTCGAGG ATGGTGAGCAGGCTGGCCCCGATGAACAGCCCCATCTGGCCCCCAATGTCACCAAGCAGC TCTGACATCTCATAGGCCTTCTTCTGCTCCACGGTCTCATAGTTGAGGGCCTCAAAGAAG ATGTCCAGGGCCAGCACGTTCTCCGCGATGTAAGCCTCGCTGCGGTTGAGCTTCCGGGCC AGGAAGCGCGCGGCGGCGCGGCTCGGGATCCGCACCATGGAGAGCTCCTTGGCGTAACGC GTGCTGGCGCACGGGTTGGGGCAGGCGCACGAGTCCTTGCGAAGCATGGCATCTATGGCC GGGTGGGCACAGTTCTTGTACTGCTGGGGGCTGCACACTGGCACGTCGCCTGGCATGTAC ACCATTCGGCAGCCGCACTTCCGAGCCACGTAACGGGTTTCGCAGGCCAGGCGACACCCC CTAAGG |
Restriction Sites | NotI-NotI |
ACCN | NM_020321 |
Insert Size | 2250 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_020321.1, NP_064717.1 |
RefSeq Size | 1766 bp |
RefSeq ORF | 1650 bp |
Locus ID | 9311 |
UniProt ID | Q9UHC3 |
Domains | ASC |
Protein Families | Druggable Genome, Ion Channels: Other |
Gene Summary | This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, two hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is an acid sensor and may play an important role in the detection of lasting pH changes. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 2 has been observed as proton-gated channels sensitive to gadolinium. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (b). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222273 | ASIC3 (Myc-DDK-tagged)-Human amiloride-sensitive cation channel 3 (ACCN3), transcript variant 2 |
CNY 4,560.00 |
|
RC222273L3 | Lenti-ORF clone of ASIC3 (Myc-DDK-tagged)-Human amiloride-sensitive cation channel 3 (ACCN3), transcript variant 2 |
CNY 6,460.00 |
|
RC222273L4 | Lenti-ORF clone of ASIC3 (mGFP-tagged)-Human amiloride-sensitive cation channel 3 (ACCN3), transcript variant 2 |
CNY 6,460.00 |
|
RG222273 | ASIC3 (tGFP-tagged) - Human amiloride-sensitive cation channel 3 (ACCN3), transcript variant 2 |
CNY 5,040.00 |