ZDHHC16 (AF258563) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APH2; DHHC-16
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF258563, the custom clone sequence may differ by one or more nucleotides ATGCGAGGCCAGCGGAGCCTGCTGCTGGGCCCGGCCCGCCTCTGCCTCCGCCTCCTTCTGCTGCTGGGTT ACAGGCGCCGCTGTCCACCTCTACTCCGGGGTCTAGTACAGCGCTGGCGCTACGGCAAGGTCTGCCTGCG CTCCCTGCTCTACAACTCCTTTGGGGGCAGTGACACCGCTGTTGATGCTGCCTTTGAGCCTGTCTACTGG CTGGTAGACAACGTGATCCGCTGGTTTGGAGTGGTGTTCGTGGTCCTGGTGATCGTGCTGACAGGCTCCA TTGTAGCTATCGCCTACCTGTGTGTCCTGCCTCTCATCCTCCGAACCTACTCAGTGCCACGACTCTGCTG GCATTTCTTCTATAGCCACTGGAATCTGATCCTGATTGTCTTCCACTACTACCAGGCCATCACCACTCCG CCTGGGTACCCACCCCAGGGCAGGAATGATATCGCCACCGTCTCCATCTGTAAGAAGTGCATTTACCCCA AGCCAGCCCGAACACACCACTGCAGCATCTGCAACAGGTGTGTGCTGAAGATGGATCACCACTGCCCCTG GCTAAACAATTGTGTGGGCCACTATAACCATCGGTACTTCTTCTCTTTCTGCTTTTTCATGACTCTGGGC TGTGTCTACTGCAGCTATGGAAGTTGGGACCTTTTCCGGGAGGCTTATGCTGCCATTGAGACTTATCACC AGACCCCACCACCCACCTTCTCCTTTCGAGAAAGGATGACTCACAAGAGTCTTGTCTACCTCTGGTTCCT GTGCAGTTCTGTGGCACTTGCCCTGGGTGCCCTAACTGTATGGCATGCTGTTCTCATCAGTCGAGGTGAG ACTAGCATCGAAAGGCACATCAACAAGAAGGAGAGACGTCGGCTACAGGCCAAGGGCAGAGTATTTAGGA ATCCTTACAACTACGGCTGCTTGGACAACTGGAAGGTATTCCTGGGTGTGGATACAGGAAGGCACTGGCT TACTCGGGTGCTCTTACCTTCTAGTCACTTGCCCCATGGGAATGGAATGAGCTGGGAGCCCCCTCCCTGG GTGACTGCTCACTCAGCCTCTGTGATGGCAGTGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF258563 unedited CACCAGCGGGTCCGGGTCCGCTGCCTGGCGCTGCGGGCGGCGGGCCATGGTGGTTTGGAT TGAGCCGGGCCCGGCCGGGGCGCCGAGTCGGAGGGGGTGGCAGTGAGCGGCGGCAGAGGC TACGGGGCTCGGTTTGGCTGACTGGGGAGTCGGCAGGCGGCAGGAACCATGCGAGGCCAG CGGAGCCTGCTGCTGGGCCCGGCCCGCCTCTGCCTCCGCCTCCTTCTGCTGCTGGGTTAC AGGCGCCGCTGTCCACCTCTACTCCGGGGTCTAGTACAGCGCTGGCGCTACGGCAAGGTC TGCCTGCGCTCCCTGCTCTACAACTCCTTTGGGGGCAGTGACACCGCTGTTGATGCTGCC TTTGAGCCTGTCTACTGGCTGGTAGACAACGTGATCCGCTGGTTTGGAGTGGTGTTCGTG GTCCTGGTGATCGTGCTGACAGGCTCCATTGTAGCTATCGCCTACCTGTGTGTCCTGCCT CTCATCCTCCGAACCTACTCAGTGCCACGACTCTGCTGGCATTTCTTCTATAGCCACTGG AATCTGATCCTGATTGTCTTCCACTACTACCAGGCCATCACCACTCCGCCTGGGTACCCA CCCCAGGGCAGGAATGATATCGCCACCGTCTCCATCTGTAAGAAGTGCATTTACCCCAAG CCAGCCCGAACACACCACTGCAGCATCTGCAACAGGTGTGTGCTGAAGATGGATCACCAC TGCCCCTGGCTAAACAATTGTGTGGGCCA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF258563 unedited NCGTCTACTATGACGGGCCGCTTTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTATCT TTAAAAATCGAATACCTTTATTTGTGCTCCCTTAAGCAGCATGTGAGAAGTGGCAGTGAC CTCAGCAGCAGGCCTGGTATCTTTGCCCTGTTGAGAAGCCAAGATCTCAGCTGTACTAGT CAGGTGTTTTTTCAGACAGCAAGTAGAAGAGGTGGTGGCCAACTCCAGTGCTGTATCCTG GAGGAGGTCCGGGTCAGCACTGGGCAAGGTAGGTAGCTAGCTGCCTGACCCCTAGTCTGG GGTTGGAACTTCTGTTTGCCTGAGTAAAGGGATGTCAGTCCTAAGATTTCTCCACATTGT GTCTTTCTTCTGCAGTGGTAAAAAGGCTGGTCCTTGAATTGTCCTGCATGGTACCCTAAG GCAGGCCCACTGGCTCTTTTTGATCAAGGATTCTGAGAAAAGCTGCCCTTGGAGGCCCTT GAAATAACATAGGGAGCAGAATGAGTGCTCGAGTCGTGGCTGACACAGTCCAGCTCACAC TGCCATCACAGAGGCTGAGTGAGCAGTCACCCAGGGAGGGGGCTCCCAGCTCATTCCATT CCCATGGGGCAAGTGACTAGAAAGTAAGAGCACCCGAGTTAGCCAGTGCCCTCCTGTTTC CACACCCAGGAATACCCTTCAGTTGTCCAAAGCACCGTAGTTGTAAAGGATTCTAAATAC TCTTGCCTTGGCCTGTAGACAACGGCTCTCCCTCTGGTGATGGGCTTTTCGAGCTGGTCT TACCTCACTGATGAGAACGCATGCCCTACATGTTGGGCACCCAGGGAAGTGGCCCAGACT GCCCGGGACCCAAGGTGACAAAACTTTGGTAGGAATC
Restriction Sites	NotI-NotI
ACCN	AF258563
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF258563.1, AAG23766.1
RefSeq Size	1747 bp
RefSeq ORF	1086 bp
Locus ID	84287
Domains	zf-DHHC
Protein Families	Transmembrane
Gene Summary	Palmitoyl acyltransferase that mediates palmitoylation of proteins such as PLN and ZDHHC6 (PubMed:28826475). Required during embryonic heart development and cardiac function, possibly by mediating palmitoylation of PLN, thereby affecting PLN phosphorylation and homooligomerization (By similarity). Also required for eye development (By similarity). Palmitoylates ZDHHC6, affecting the quaternary assembly of ZDHHC6, its localization, stability and function (PubMed:28826475). May play a role in DNA damage response (By similarity). May be involved in apoptosis regulation (By similarity). Involved in the proliferation of neural stem cells by regulating the FGF/ERK pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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