Apg12 (ATG12) (BC012266) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APG12; Apg12 (autophagy, yeast) homolog; APG12 autophagy 12-like; APG12L; ATG12 autophagy related 12 homolog (S. cerevisiae); FBR93; HAPG12
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC012266 edited GAATTCGGCACCAGGTCCGAGCCAGCGGCCTAACTGGAGTGTGCGCATTCGACCGAGCAC AGACACGTTGCCCACCGCTCCTCTCCCGAGGTCTGTAGTCGCGGAGAAACACATGTTGCG TTACTAACGTTCAGAGGTCTGCGACAGCTTCGATTTGAATGACTAGCCGGGAACACCAAG TTTCACTGTGTAATTGCGTCCCCCTACTCCGGCGCCTCCTTTGCGACGCTCCCTGGAGAA AAGCACGCCCACTGCACGCGCTCAGTCGCTACTTCCGCTCTCGAGTGTCTCCAAGCAAGA TGGCGGAGGAGCCGCAGTCTGTGTTGCAGCTTCCTACTTCAATTGCTGCTGGAGGGGAAG GACTTACGGATGTCTCCCCAGAAACAACCACCCCGGAGCCCCCGTCTTCCGCTGCAGTTT CCCCGGGAACAGAGGAACCTGCTGGCGACACCAAGAAAAAAAATTGACATTTTGCTAAAG GCTGTGGGAGACACTCCTATTATGAAAACAAAGAAGTGGGCAGTAGAGCGAACACGAACC ATCCAAGGACTCATTGACTTCATCAAAAAGTTTCTTAAACTTGTGGCCTCAGAACAGTTG TTTATTTATGTGAATCAGTCCTTTGCTCCTTCCCCAGACCAAGAAGTTGGAACTCTCTAT GAGTGTTTTGGCAGTGATGGTAAACTGGTTTTACATTACTGCAAGTCTCAGGCGTGGGGA TGAACCACAAAGAAAATCAACTTGCTACTACATGAAATGGATTTTCACGGAAGAGACAGC TCTGAAAAGTTTTGATGCTTGTGGCAAGAGACTTAACAGATGTGAXXXXXXXXXXXXXXC TCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012266 unedited NGGAACCGTACAAAAATTTGTAAACGACTCATATAGGCGGCCGCGNAATTCGGCACCAGG TCCGAGCCAGCGGCCTAACTGGAGTGTGCGCATTCGACCGAGCACAGACACGTTGCCCAC CGCTCCTCTCCCGAGGTCTGTAGTCGCGGAGAAACACATGTTGCGTTACTAACGTTCAGA GGTCTGCGACAGCTTCGATTTGAATGACTAGCCGGGAACACCAAGTTTCACTGTGTAATT GCGTCCCCCTACTCCGGCGCCTCCTTTGCGACGCTCCCTGGAGAAAAGCACGCCCACTGC ACGCGCTCAGTCGCTACTTCCGCTCTCGAGTGTCTCCAAGCAAGATGGCGGAGGAGCCGC AGTCTGTGTTGCAGCTTCCTACTTCAATTGCTGCTGGAGGGGAAGGACTTACGGATGTCT CCCCAGAAACAACCACCCCGGAGCCCCCGTCTTCCGCTGCAGTTTCCCCGGGAACAGAGG AACCTGCTGGCGACACCAAGAAAAAAAATTGACATTTTGCTAAAGGCTGTGGGAGACACT CCTATTATGAAAACAAAGAAGTGGGCAGTAGAGCGAACACGAACCATCCAAGGACTCATT GACTTCATCAAAAAGTTTCTTAAACTTGTGGCCTCAGAACAGTTGTTTATTTATGTGAAT CAGTCCTTTGCTCCTTCCCCAGACCAAGGAGTTGGAACTCTCTATGAGTGTTTTGGCAGT GATGGTAAACTGGTTTTACATTACTGCAAGTCTCAGGGCGTGGGGATGAACCACAAAGAA NATCAACTTGCTACTACATGAAATGGATTTTCACGGAAGAGACAGCTCTGGAAAGTTTTG ATGCTTGTGGNCAGAGACTCTACAGATGTGATCTATCTAGTATGTGTCTAC
Restriction Sites	NotI-NotI
ACCN	BC012266
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC012266.2, AAH12266.2
RefSeq Size	912 bp
RefSeq ORF	564 bp
Locus ID	9140
Domains	APG12
Protein Pathways	Regulation of autophagy, RIG-I-like receptor signaling pathway
Gene Summary	Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

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