KLHL7 (AL136597) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KLHL6; SBBI26
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL136597, the custom clone sequence may differ by one or more nucleotides ATGGCAGCCTCTGGGGTGGAGAAGAGCAGCAAGAAGAAGACCGAGAAGAAACTTGCTGCTCGGGAAGAAG CTAAATTGTTGGCGGGTTTCATGGGCGTCATGAATAACATGCGGAAACAGAAAACGTTGTGTGACGTGAT CCTCATGGTCCAGGAAAGAAAGATACCTGCTCATCGTGTTGTTCTTGCTGCAGCCAGTCATTTTTTTAAC TTAATGTTCACAACTAACATGCTTGAATCAAAGTCCTTTGAAGTAGAACTCAAAGATGCTGAACCTGATA TTATTGAACAACTGGTGGAATTTGCTTATACTGCTAGAATTTCCGTGAATAGCAACAATGTTCAGTCTTT GTTGGATGCAGCAAACCAATATCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAACAA GTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTAGCGGAGTGTCTAGATTGTCCTGAATTGAAAGCAA CTGCAGATGACTTTATTCATCAGCACTTTACTGAAGTTTACAAAACTGATGAATTTCTTCAACTTGATGT CAAGCGAGTAACACATCTTCTCAACCAGGACACTCTGACTGTGAGAGCAGAGGATCAGGTTTATGATGCT GCAGTCAGGTGGTTGAAATACGATGAGCCTAATCGCCAGCCATTTATGGTTGATATCCTTGCTAAAGTCA GGTTTCCTCTTATATCAAAGAATTTCTTAAGTAAAACGGTACAAGCTGAACCACTTATTCAAGACAATCC TGAATGCCTTAAGATGGTGATAAGTGGAATGAGGTACCATCTACTGTCTCCAGAGGACCGAGAAGAACTT GTAGATGGCACAAGACCTAGAAGAAAGAAACATGACTACCGCATAGCCCTATTTGGAGGCTCTCAACCAC AGTCTTGTAGATATTTTAACCCAAAGGATTATAGCTGGACAGACATCCGCTGCCCCTTTGAAAAACGAAG AGATGCAGCATGCGTGTTTTGGGACAATGTAGTATACATTTTGGGAGGCTCTCAGCTTTTCCCAATAAAG CGAATGGACTGCTATAATGTAGTGAAGGATAGCTGGTATTCGAAACTGGGTCCTCCGACACCTCGAGACA GCCTTGCTGCATGTGCTGCAGAAGGCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCAGCTCT GTATTTATTTGAGTGCTATGATACGAGAACTGAAAGCTGGCACACAAAGCCCAGCATGCTGACCCAGCGC TGCAGCCATGGGATGGTGGAAGCCAATGGCCTAATCTATGTTTGTGGTGGAAGTTTAGGAAACAATGTTT CAGGGAGAGTGCTTAATTCCTGTGAAGTTTATGATCCTGCCACAGAAACATGGACTGAGCTGTGTCCAAT GATTGAAGCCAGGAAGAATCATGGGCTGGTATTTGTAAAAGACAAGATATTTGCTGTGGGTGGTCAGAAT GGTTTAGGTGGTCTGGACAATGTGGAATATTACGATATTAAGTTGAACGAATGGAAGATGGTCTCACCAA TGCCATGGAAGGGTGTAACAGTGAAATGTGCAGCAGTTGGCTCTATAGTTTATGTCTTGGCTGGTTTTCA GGGTGTTGGTCGATTAGGACACATTCTCGAATATAATACCGAAACAGACAAATGGGTTGCCAACTCCAAA GTTCGTGCTTTTCCAGTCACAAGTTGTTTAATTTGTGTTGTCGATACTTGTGGAGCAAATGAAGAGACCC TTGAAACATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL136597 unedited GCATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTAGCGTCGC TCCCTGAGCGTTTCTAGGGGGCCGCCCGGCCTTGTCTTTCGGCAGTGGCCGAGCCACCGC CGCCTGCCGCGCGTTCCAGAGCTGGGCGCTGCAGCTGCACTGCCGATCGCCGTGTTTGGT CGATAGAATCCCCAGTGTGCCCAGAGAGTGCGACCCCTCGCCCGGCCCGGCGAGCCCCGG GCGTGAACCGAGCTGAGGGAGGATGGCAGCCTCTGGGGTGGAGAAGAGCAGCAAGAAGAA GACCGAGAAGAAACTTGCTGCTCGGGAAGAAGCTAAATTGTTGGCGGGTTTCATGGGCGT CATGAATAACATGCGGAAACAGAAAACGTTGTGTGACGTGATCCTCATGGTCCAGGAAAG AAAGATACCTGCTCATCGTGTTGTTCTTGCTGCAGCCAGTCATTTTTTTAACTTAATGTT CACAACTAACATGCTTGAATCAAAGTCCTTTGAAGTAGAACTCAAAGATGCTGAACCTGA TATTATTGAACAACTGGTGGAATTTGCTTATACTGCTAGAATTTCCGTGAATAGCAACAA TGTTCAGTCTTTGTTGGATGCAGCAAACCAATATCAGATTGAACCTGTGAAGAAAATGTG TGTTGATTTTTTGAAAGAACAAGTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTAGC GGAGTGTCTAGATTGTCCTGATTGAAAGCACTGCAGATGACTNTATTCATCAGCACTTTT ACTGAGTTTACAAAACTGATGAAATTCTTCAACTTGATGTCAAGCGAGTAACACATCTTC TCACCCAGACACTCTGACTGTGNAGAGCAGAGGATCAGGNT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL136597 unedited NCCCCAGGGTCGCTGACCCAATCCANGTTCAGTTTTATTTCTTTTTTTTTAAGTTAAAAA AATTTAATATTATCAGATAAAAACATTGATTGTGACAGCTTTACATGTTTCATTCCATAC ACANGTGAAAAAGGCAAAGTATTACTAGGAAGGAAATGAGTAGTTGGGTTTGTAAAAGAT TTATTGATCACATAAAAGCAATGAATAGGAGATGACATATATCTAACTCATGATTACAAA ACTTTTAGAGACAACTAGGTTAAATATTTCATGTTTTGGCTAAGGGAAAAGAATTCATGA TCTCGTTAAAGAAGTTCAAAGTCAACACTGATATTGTACAACTACCAGTGCTTTTCTAAT GATCTAGAAATTTGCTATAAATAGCTTTACTTTTTGCTAGTTAATCACTTACTATGATTG GGTTTTATTTAACAGTGAGGACTAAAGAGGCAGAATCCTTTTTTAACCACTGGATAAAAC AGAGACATAAATTAATGATGGGTGCTAAGCTTTACATTTTATGTTAAAAAGATGGAATAT ATTTAAGTGGATTGAATATATTTCATGAGGAGTTGGACATATTAAACCCCCAAATCTGTT TAGGAAGTTTTGTGAGAAAGTGTGTATATGCCTTGACTCACTCTGGCTTACTTTTAAAAT GGAATACATTTGGCACTAAATAAAATAAATAGACCAGCCAACTGTGAGACTAAGCTGCTC CATCTGAGTTGCCCATCTTGACTTACAATTCATACTATTTCTTAGGTCTTGACTGAGCAC AAACAGACCTAACCGTATAGCACTTCTGTGATGCATGAAAATAGAATCTAGAGCTAAATA CTGCGCTCACTGATGAGCCAATCTTCTTTCTGCCAAAATCATAGTTCAATGTGAAATCAT TCAGAATATCCATTTTTAACAAAGCTTATTCTTTTTC
Restriction Sites	NotI-NotI
ACCN	AL136597
Insert Size	3300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL136597.1, CAB66532.1
RefSeq Size	3028 bp
RefSeq ORF	3028 bp
Locus ID	55975
Domains	BTB, Kelch
Gene Summary	This gene encodes a BTB-Kelch-related protein. The encoded protein may be involved in protein degradation. Mutations in this gene have been associated with retinitis pigmentosa 42. [provided by RefSeq, Feb 2010]

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