GALNS (BC018027) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GalN6S; GALNAC6S; GAS; MPS4A
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC018027, the custom clone sequence may differ by one or more nucleotides GTCGAGGCCCCGCCCCGCAGCCCAGCCGGAAGGGCCGGCGGACGCTCGCTAGGTCGGCTCGCTGGCCGGG GCTCCGCGGCTCCCGTGGTTGCCATGGCGGCGGTTGTCGCGGCGACGAGGTGGTGGCAGCTGTTGCTGGT GCTCAGCGCCGCGGGGATGGGGGCCTCGGGCGCCCAGCAGCCCCCCAACATCCTGCTCCTGCTCATGGAC GACGAATCACTCCCGCCACCTCGGGCCGCCGGGTCCCCCCGCTGGGCTCGAGTCTTACCTGACGGCTGTC TCTGTCCTCTGGAGAATGCTGAGCGAAGTGACTGCTTTCCAGAGCACGCTCTCCAGCGCCCAGGGGTCCG TGTCCTGACAGCAGTAGCCGGGCCTTTCTCTGCACTGAGTGGCATCTCCCTCCTAAAGATACTTTGCTTC AGAATTTCATCCTTTCACTTTTGAAGTTCCTCTGGGCCAGTGTAGATACTTTGTTTCAGAATTTCTTCTT TTCATTGTGTAACTCCTTCTGGGCCAGCATAGCTTCGCTGGATGAGCTGGGCTCTGCAGGTGAAGCCTCC CTGTTAAGTTTCTGCTTTTCCTGACTTTCTCAGAGGACAGAGGGGTGAGGCGGGGCAGCTCATGAATAAT TTTTTATGTTGAAGACTTGTCGAAATAATATTTTGGTTATGTTGGGTTAAATAAAATATAAAGTTAGTCT GTAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC018027 unedited GGGGGGNGGGGNNNNNNNNNNNNNCCCNNNNCCGGTTCAGAATTTGTATACGACTCACTA TAGGCGGCCGCGNATTCGGCACCAGGCTAGGTCGGCTCGCTGGCCGGGGCTCCGCGGCTC CCGTGGTTGCCATGGCGGCGGTTGTCGCGGCGACGAGGTGGTGGCAGCTGTTGCTGGTGC TCAGCGCCGCGGGGATGGGGGCCTCGGGCGCCCCGCAGCCCCCCAACATCCTGCTCCTGC TCATGGACGACATGAAGGATTTGGGGGCAGAGCACTTGGCAGGTCATGAAGGGGTCCAAC TTCTCGGGTTGTTGAACGTCTACCTGGAACAAGAAGAGAGATTCCAACCTCGAGAAAAAG GGCTGAGTTTGATTGAGGCTACCCCGGAGAATGATAACACTTTGTGTCCAGGATTGAGAA ATGCCAAAGTTGAAGATTTAAGGAGTTTAGCCAACTTTTTTGGATCTTGCACTGAAACTT TTGTCCTGGCTGTCAATATTTTGGACAGGTTCTTGGCTCTTATGAAGGTGAAACCTAAAC ATTTGTCTTGCATTGGAGTCTGTTCTTTTTTGCTGGCTGCTAGAATAGTTGAAGAAGACT GCAATATTCCATCCACTCATGATGTGATCCGGATTAGTCAGTGTAAATGTACTGCTTCTG ACATAAAACGGATGGAAAAAATAATTTCAGAAAAATTGCACTATGAATTGGAAGCTACTA CTGCCTTNAACTNTNTGCACTTATACCATACTATTATACTNTGTCATACTTCAGAAAGGA AGAAATACTGAGCCTTTGATAACTAGAAGCTCAGCTNGAAGCTTTGCACTGCCGACTCAT CTTTTCAAAGCAAACCATCTGTATTAGCCTTGTGCCTCTCATTTTTGAAGTGGAAACTTT GAAAATCTGTGNAATACTGGNAANTCTCTTGCTAGTTAA
Restriction Sites	NotI-NotI
ACCN	BC018027
Insert Size	2650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC018027.1
RefSeq Size	737 bp
RefSeq ORF	737 bp
Locus ID	2588
Protein Families	Druggable Genome
Protein Pathways	Glycosaminoglycan degradation, Lysosome, Metabolic pathways
Gene Summary	This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq, Jul 2008]

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