SF3B2 (BC010372) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC010372, the custom clone sequence may differ by one or more nucleotides GGGGGCGCCTTCCTGCGGCTAAGATGGCGACGGAGCATCCCGAGCCTCCCAAAGCAGAATTGCAGCTGCC GCCGCCGCCACCTCCAGGCCACTATGGCGCCTGGGCTGCCCAGGAGCTTCAGGCCAAGTTGGCAGAGATC GGAGCTCCGATCCAGGGTAATCGCGAGGAGCTGGTGGAGCGGCTGCAGAGCTACACCCGCCAGGTAGGTG TTGGCGGCGGGACGTCAGGATAGGCCGAGCTTCTCCAGGAGACCTTATATACCCCATCACGGCTCGGTCT TCATTCTTTCTTTATCTCGGCACAGCAAGGCGGAGGCTTGCCCTTTTTAGCTTGTTCTTGCCTTTGCCAG CTTAGTTGTAATTTCTTGTATCCATCTTGGTCCTCTTCAGTGCCCAGCCAGAGCGCTGGCAGACAGGCAC TGGGTACGTTTTGTTGAATGAATTGGGAGCGAACGTCGTTTAGTGAAACAGCTAGTTTTGGACTGACCTA GAGTCCTTTCTCTTTTGCAGACTGGCATCGTGCTGAATCGGCCGGTTTTGAGAGGGGAAGATGGGGACAA AGCCGCTCCACCTCCCATGTCGGCACAGGTAGGGAGATTCTTCTGTTTTTTAAGATTCCATCTGCTGATC CTTTTGTAGTTCATGAGCATGATGATTGGGTGTTCACGTGCATGTGTGAGATGTGACACCCTTGCACATT ACTCGCCTGACCTGAGTGGGGAAAAAAAAAGATTCCATATGTTCAGGGTAGCCCTTCCTCTCACTTGCAG TTGTTGGGCAGGGAGGTACTGCCATGACAGAATTGTGAGCTCTTCAGTTCTCTCTTTGATGCTGACACTT GCCTCCTTTTCCACTGTCTGCTTCTCCAAAAGCAGCTTGTGGCCGGGTGCAGTGGCGCATGCCTGTAATC CCACCACTTCGGGATGCTTAGATGGGACGATCGCTTGAAGCCAGGAGTTCGAGACCAGCCTGGGCAACAT AGTGAGACTCTGTTACTACAAAAAATTTTAAAAATTAGCTGGGCACGGTGGCACGCGCCTATAAAGTCCC TATAAAGAGGCTCAAGGATTGCTGGAGCCCTGGGGTTGGAGGCTGCAGTGAGCTCTGGTTGCGCCACTGT ACTCCATGTACTCCATCCTGGGTGACAGAATGAGACTTTGCTTCTTTAAAAAAAAAAGGGGGGAGGTGAT GGGGGAGGGGCTGGGAGAGTGTCAGAAAAAATAGCTAATGCATGCCAGGCTTAATACTTAGGCAGTGGGT TGATAGGTGCAGCAAACCACCATAGCACACATTTACCTATGTAACAAACCTGCGCATACTGCACATGTAC CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010372 unedited TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGCGCGCCTTCCT GCGGCTAAGATGGCGACGGAGCATCCCGAGCCTCCCAAAGCAGAATTGCAGCTGCCGCCG CCGCCACCTCCAGGCCACTATGGCGCCTGGGCTGCCCAGGAGCTTCAGGCCAAGTTGGCA GAGATCGGAGCTCCGATCCAGGGTAATCGCGAGGAGCTGGTGGAGCGGCTGCAGAGCTAC ACCCGCCAGACTGGCATCGTGCTGAATCGGCCGGTTTTGAGAGGGGAAGATGGGGACAAA GCCGCTCCACCTCCCATGTCGGCACAGCTCCCTGGAATTCCCATGCCACCACCACCTTTG GGACTCCCCCCTCTGCAGCCTCCTCCGCCACCCCCACCACCTCCACCAGGCCTTGGCCTT GGCTTTCCTATGGCCCACCCACCAAATTTGGGGCCCCCGCCTCCTCTCCGTGTGGGTGAG CCAGTGGCACTGTCAGAGGAGGAGCGGCTGAAGTTGGCTCAGCAGCAGGCGGCATTGCTG ATGCAGCAGGAGGAGCGTGCCAAGCAGCAGGGAGATCATTCGCTGAAGGAACATGAGCTC TTGGAGCAGCAGAAGCGGGCAGCTGTGTTACTGGAGCAGGAACGACAGCAGGAGATTGCC AAGATGGGCACCCCAGTCCCTCGGCCCCCACAAGACATGGGCCAGATTGGTGTGCGCACT CCTCTGGGTCCTCGAGTAGCTGCTCCAGTGGGCCCCAGTGGCCCCCTTCTACAGTTCGCC CATGGGGAGCCCCTGTTCCCGGCCCTCGTGTCCCCCACCGCCCCTGGAGAGAGACAGAGA GATGAGACCCCTCTGGGGCCCCAGATCCCCAGCTTGGGAAAAATCTGCACTGAAGAAAGC GCAGNANNAATGATCTCACAGAGAGAAAGAATGGAACGAGCCTCTCC
Restriction Sites	NotI-NotI
ACCN	BC010372
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC010372.1
RefSeq Size	1362 bp
RefSeq ORF	1362 bp
Locus ID	10992
Protein Pathways	Spliceosome
Gene Summary	This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq, Jul 2008]

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