Eph receptor A4 (EPHA4) (AF075040) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HEK8; SEK; TYRO1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF075040, the custom clone sequence may differ by one or more nucleotides TGCTATCTTGGCCTCACAGCCCTTCCTGTTGATTACAAAGCCCGTGGAAGAAAACAGAACACACCCTCCT CAGTTCCGTCTAAATGTGTTTCTTCTGCTTCAATTACACCAGTTCTGGGGCAAAGACACTGATGAAACAA CACCCATACCTGAAAAGAATAAATGTGTGACTTTCAAATCCCCTTTCGCAGTGAAAGAAACAGCAAACAC TTAAGATTCAGCATCTGTTCTCCAGTTGCACTGAGGAATGCACTGTCTCGCAGCACCAGCTCTGCAGAGC CCTTGCCCCAGACTCTTTGCGGTTTTATTTATATGTATTTCCATATTTCATTCCTGTGTGTCACTGCTGC ATTGGTGTGGCAGCAAGTGACCAAATGCTACAGGTCTTACTATGGACACCAGGTCAGGTGCAACCACACA AAACAAAGCCAGTTCCATGAGCTGCCTATGATATGCATTGCGGAAGTAACATTTTACCCAGGGTGTGCCA TTGCAGTGATATAAATATATTTTTTTCTTAGACTAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF075040 unedited TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACACTTGTCCTAACTATCTT GTGGATAGTGGGCTGTGACAATCTGGAATAGAGAACGTTCACACTTCGCTCCTTTAAAGA AGCGACCCCAGATCTGCAAGGGAGTAGATTCTGCTATCTTGGCCTCACAGCCCTTCCTGT TGATTACAAAGCCCGTGGAAGAAAACAGAACACACCCTCCTCAGTTCCGTCTAAATGTGT TTCTTCTGCTTCAATTACACCAGTTCTGGGGCAAAGACACTGATGAAACAACACCCATAC CTGAAAAGAATAAATGTGTGACTTTCAAATCCCCTTTCGCAGTGAAAGAAACAGCAAACA CTTAAGATTCAGCATCTGTTCTCCAGTTGCACTGAGGAATGCACTGTCTCGCAGCACCAG CTCTGCAGAGCCCTTGCCCCAGACTCTTTGCGGTTTTATTTATATGTATTTCCATATTTC ATTCCTGTGTGTCACTGCTGCATTGGTGTGGCAGCAAGTGACCAAATGCTACAGGTCTTA CTATGGACACCAGGTCAGGTGCAACCACACAAAACAAAGCCAGTTCCATGAGCTGCCTAT GATATGCATTGCGGAAGTAACATTNTACCCANGGGTGTGCNCATTGCAGTGATATAAATA TATTNTTTTCTTAGACTAAATATGAGCTGACTATCTCTTTTGATGTGTGTACATANNNGT GTGAGTGTGTCTGTATGCGTGCCTGTCTGTGTGCCGGNTGTGTGTATGTGCGTANCCTCA TGCTTANGACTACCCATGAATGTGTGGATGCTACACTNGNAGAGTTCTGGTTTNCCCCAG TTNCAGATGGAAGACTACTGATACAGTGGACCTGGAGACCATCCCCTTGAANNGACACCC NNAATGTTCAGCATCCTGGATCTACCCCTNCTGGATTTACACCGGTTTTTTGTANCTGTC ACACTACCTTATAGAATTCTAGCN
Restriction Sites	NotI-NotI
ACCN	AF075040
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF075040.1
RefSeq Size	534 bp
RefSeq ORF	534 bp
Locus ID	2043
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Axon guidance
Gene Summary	This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

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