UBE2W (BC010900) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	6130401J04Rik; FLJ11011; ubiquitin-conjugating enzyme E2W (putative)
Vector	PCMV6-Neo
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC010900 edited GGGGATCATGGCGTCAATGCAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGA CCCATCTCCTGGAATGACCTTAAATGAGAAGAGTGCTCAAAATTCAATTACACAGTGGAT TGTAGACATGGAAAGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATT TAAATTTAGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATAT TCCTGTTCATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGA CTGGTCCCCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAG CTGCAAGGAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAA TCCAAAGAAAACAAAATGGTGGTATCATGAACTGAAATCTGCCTTCATACTATCTATTAC GGATTGAATCATGTCCTCCAATAAGATGTGTTGAAGTCTTAACTCCCAGTACCTCAAATT GTGACCCTATTTGGAAATAGAGTCATTGCAGATGTCATTAGTTGAGGTGAGGTTATACCG AAGTAGAATGTGGCCTTAATCCAATATAACTGGTGTCCTAAGAATTGGAGAAGAGACTCA GACACATGGGGAGAAGACCATGCAGTCATGGGGTGATGTATCAACAAGCCAGCAAATGCC AGGGATTGCTGGGAACATCAGAGGCTGGAAGAGGCAGGGAAATGTTCTTCCCTAGAGCTG TGGGAGAGAACATGATTCTGCTGACACCTTGATCATGGACTTCCAGCCTCCAGAACTGTG AGACAATAAATGTCTGGTTTCTTTTAAGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	NotI-NotI
ACCN	BC010900
Insert Size	900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010900.1, AAH10900.1
RefSeq Size	899 bp
RefSeq ORF	899 bp
Locus ID	55284
Domains	UBCc
Protein Families	Transcription Factors
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

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