SNRPN (AF319523) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HCERN3; PWCR; RT-LI; SM-D; sm-N; SMN; SNRNP-N; SNURF-SNRPN
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF319523, the custom clone sequence may differ by one or more nucleotides ATGACTCCACAGGGAAGAGGCACTGTAGCAGCTGCTGCTGTTGCTGCGACTGCCAGTATTGCTGGAGCCC CAACACAGTACCCACCAGGACGGGGCACTCCGCCCCCACCCGTCGGCAGAGCAACCCCACCTCCAGGCAT TATGGCTCCTCCACCTGGTATGAGACCACCCATGGGCCCACCAATTGGGCTTCCCCCTGCTCGAGGGACG CCAATAGGCATGCCGCCTCCGGGAATGAGACCCCCTCCACCAGGCATTAGAGGGGAAGCATTTCTCCTTG CATTGGTTTGCTGGTATGGATGGCCCTTAGATTTTGGCATGAGTGATTATGCAGCCTATTTGAAGAAAAC CTGTTTTCTAGGACCTTTTTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF319523 unedited CACGAGGCTGGTAGAGGAGTACCAGCTGGTGTGCCAATTCCCCAGGCCCCTGCTGGATTG GCAGGCCCTGTCCGAGGAGTTGGGGGACCATCCCAGCAGGTAATGACTCCACAGGGAAGA GGCACTGTAGCAGCTGCTGCTGTTGCTGCGACTGCCAGTATTGCTGGAGCCCCAACACAG TACCCACCAGGACGGGGCACTCCGCCCCCACCCGTCGGCAGAGCAACCCCACCTCCAGGC ATTATGGCTCCTCCACCTGGTATGAGACCACCCATGGGCCCACCAATTGGGCTTCCCCCT GCTCGAGGGACGCCAATAGGCATGCCGCCTCCGGGAATGAGACCCCCTCCACCAGGCATT AGAGGTCCACCTCCCCCAGGAATGCGTCCACCAAGACCTTAGCATACTGTTGATCCATCT CAGTCACTTTTTCCCCTGCAATGCGTCTTGTGAAATTGTGTAGAGTGTTTGTGAGCTTTT TGTTCCCTCATTCTGCATTAATAATAGCTAATAATAAATGCATAGAGCAATTAAACTGTG AAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAA CAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTG CCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF319523 unedited TTGTACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTCAAGTTTAATTGCTC TATGCATTTATTATTAGCTATTATTAATGCAGAATGAGGGAACAAAAAGCTCACAAACAC TCTACACAATTTCACAAGACGCATTGCAGGGGAAAAAGTGACTGAGATGGATCAACAGTA TGCTAAGGTCTTGGTGGACGCATTCCTGGGGGAGGTGGACCTCTAATGCCTGGTGGAGGG GGTCTCATTCCCGGAGGCGGCATGCCTATTGGCGTCCCTCGAGCAGGGGGAAGCCCAATT GGTGGGCCCATGGGTGGTCTCATACCAGGTGGAGGAGCCATAATGCCTGGAGGTGGGGTT GCTCTGCCGACGGGTGGGGGCGGAGTGCCCCGTCCTGGTGGGTACTGTGTTGGGGCTCCA GCAATACTGGCAGTCGCAGCAACAGCAGCAGCTGCTACAGTGCCTCTTCCCTGTGGAGTC ATTACCTGCTGGGATGGTCCCCCAACTCCTCGGACAGGGCCTGCCAATCCAGCAGGGGCC TGGGGAATTGGCACACCAGCTGGTACTCCTCTACCAGCCTCGTGCCGAATTCGCGGCCGC CCTATAGTGAGTCGTATTTACAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAG ACCTCCCACCGTACACGCCTACCGGCCAATTGCGTCAACGGGGCGGGGTTATTACGACAT TTTTGGAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTTCATGGGGTTG AGACTTGGAAATCCCCGTGAATCAAACCGCTTTCCACGCCCATTGGGGTACTGCCAAAAC CGGCTTCCCCTGGTTAATAGCGATGACTAATACCTTAAAGTTCTGCCCATTAGGAAAGTC CGGTAAGGCCTGTTCCTGGGCATAATGGCAGGCGGGCCCATTTACGGTCATTGACGTTCA ATAGGGGCCGGACTTTGGAA
Restriction Sites	NotI-NotI
ACCN	AF319523
Insert Size	600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF319523.1, AAK18178.1
RefSeq Size	588 bp
RefSeq ORF	588 bp
Locus ID	6638
Protein Families	Stem cell - Pluripotency
Gene Summary	This gene is located within the Prader-Willi Syndrome critical region on chromosome 15 and is imprinted and expressed from the paternal allele. It encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing and may contribute to tissue-specific alternative splicing. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants that initiate at the CpG island-associated imprinting center may be bicistronic and also encode the SNRPN upstream reading frame protein (SNURF) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (SNHG14) may originate from the promoters at this locus and share exons with this gene. Alterations in this region are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]

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