TRIM14 (AK097480) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC105060 sequence for AK097480 edited (data generated by NextGen Sequencing) ATGAATGATCTCTCCAACAGGGTCTGGAGTATCAGCCAGGAGCCCGATCCTGTCCAGAGG CTTCAGGCATACACGGCCACCGAGCAGGAGATGCAGCAGCAGATGAGCCTCGGGGAGCTG TGCCATCCCGTGCCCCTCTCCTTTGAGCCCGTCAAGAGCTTCTTTAAGGGCCTCGTGGAA GCCGTGGAGAGTACATTACAGACGCCATTGGACATTCGCCTTAAGGAAAGCATAAACTGC CAGCTCTCAGACCCTTCCAGCACCAAGCCAGGTACCTTGTTGAAAACCAGCCCCTCACCA GAGCGATCGCTATTGCTGANNGCCTGCTGGGCAGCCTGGGGCCCGTGCCCGTGCTGCGGT TCGACGCGCTCTGGCAAGTGCTGGCTCGTGACTGCTTCGCCACCGGCCGCCACTACTGGG AGGTTGACGTGCAGGAGGCGGGCGCCGGCTGGTGGGTGGGCGCGGCCTACGCCTCCCTTC GGCGCCGCGGGGCCTCGGCCGCCGCCCGCCTGGGCTGCAACCGCCAGTCCTGGTGCCTCA AGCGCTACGACCTTGAGTACTGGGCCTTCCACGACGGCCAGCGCAGCCGCCTGCGGCCCC GCGACGACCTCGACCGGCTCGGCGTCTTCCTGGACTACGAGGCCGGCGTCCTCGCCTTCT ACGACGTGA Clone variation with respect to AK097480.1 317 c=>t;320 t=>n;321 c=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK097480 unedited CGGATTTTGTAACACGATTTACTATAGNNGGCGGCACGCGCCACCGNANAAGCTCAAGGC TAATGCAGAGTCAAGTAAAACCTGGCTGAAGGGGAAATTCACTGAACTCAGATTACTACT TGACGAAGAGGAAGCGCTGGCCAAGAAATTCATTGATAAAAACACGCAGCTTACCCTCCA GGTGTACAGGGAACAAGCTGACTCTTGCAGAGAGCAACTTGACATCATGAATGATCTCTC CAACAGGGTCTGGAGTATCAGCCAGGAGCCCGATCCTGTCCAGAGGCTTCAGGCATACAC GGCCACCGAGCAGGAGATGCAGCAGCAGATGAGCCTCGGGGAGCTGTGCCATCCCGTGCC CCTCTCCTTTGAGCCCGTCAAGAGCTTCTTTAAGGGCCTCGTGGAAGCCGTGGAGAGTAC ATTACAGACGCCATTGGACATTCGCCTTAAGGAAAGCATAAACTGCCAGCTCTCAGACCC TTCCAGCACCAAGCCAGGTACCTTGTTGAAAACCAGCCCCTCACCAGAGCGATCGCTATT GCTGAAATACGCGCGCACGCCCACGCTGGATCCTGACACGATGCACGCGCGCCTGCGCCT GTCCGCCGATCGCCTGACGGTGCGCTGCGGCCTGCTGGGCAGCCTGGGGCCCGTGCCCGT GCTGCGGTTCGACGCGCTCTGGCAAGTGCTGGCTCGTGACTGCTTCGNCACCGGCCGCCA CTACTGGGAGGTTGACGTGCANGAAGCGGGCGCCNGCTGGNTGGTGGGCGCGGCCTACGC NTCCTTTCGCGCCGCGGGGCCTCGGCCCGCGCCCGCTGGGCTGCACCGCCAGTCCTGGTG CCTCAGCGCTACGACCTTGATACTGGGCCTCCACGACGGCAGNGCAGCGCCTGCGGNCCG CGACGACTCGACCGGNTGGCGTNTTCTGGATACAGGCCGGGTCCTGCCTCTAGAGTGAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK097480 unedited CATGGATCCTGACACGAATTCAGGTCTAGTTTTAATTTCTTTTTTTTGTTATTTCATGGG AAACTCCTGGAAGCCTTTTCATAGCTGAGGGGTTAAGTGAAGTTTTAGGAAGATCACTCT AGTTTTAGGGTGGAAGATGAGGACTGGGAGAGGGTTCAGACCAGGAACTGCTAAGAAGCT CTTGCAGCTTCTGGAGGGAGCTGTGATGTGGACACGCTGAAATCGGTGGGGAGGAAGCGG AAGATTCAAGGGACCTCAAGAGACAGAAGAGTCAGGACTGGATGACTCCCATTCATTCAC AAACGTTTACTGTGTGCCTACTATGTGTCAGGTTCCTGTGCTGTACGAGGGAGAAGGTTC CCAGGCTGCGGATGATGTATTCAGGATGCTGAGGGCTTACCTGTGAGGGTATCACTTTGA AGGAACTGGATTAAAGTAGTGTAAGTGATGGTGGGGTGAGGGTGCGGAGGTCTGATGAGA GGGCAGCAGCAGACTTGTTTAGGGCCTGTTTGAAACTAGCCTAGGAGAGGAAACCTTCAA AGCACTGTANGCGTGATTGGTCGGGGAAAGCTGGGGCAGGGAGAGGGCCCTAAGAAGCAN GCAGTAAGGGGACCAGCCACGCGGATCTAGGTAGATTANGCGAGACTGGGCAGCTGCGGC GTACCTGGAGGCTGTCACGCCGGTCCCTGGCCCCCTAGGCAGCCGGGGGGATGCTGATGG CCCCCTCCCAGAGCCGCAGGGCCGGGTAGAGCCGCTCCTGGAACGTGGCGCCGGAAGTAT GCCAGTGGCTCATGCCCGCCCGCACGTCGTAAAAGGGGAGGACGCCCGGCCTCTTATTCA GGAAGACCCCGAACCGTTCCAGGTCTTCCGCGGGCCGCAGGGCGCTTGGCCTGACCTGTC CTGGAAGGCCCAATCTCAAGGTTCTAACGGCTTGAGGGACCAGACCTGGGGGTTGTAGCC CCG
Restriction Sites	NotI-NotI
ACCN	AK097480
Insert Size	1700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK097480.1, BAC05071.1
RefSeq Size	2387 bp
RefSeq ORF	669 bp
Locus ID	9830
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies and its function has not been determined. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

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