MAP3K4 (AK094629) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC103206 sequence for AK094629 edited (data generated by NextGen Sequencing) ATGGCGGCGGCAGGCGTGACGGCAAAGGCGGGAGGAGGGACCAGCGCGGCAGCGGCTTCT CTCATCCGTGCACGGAGCCCCGCATGGCCGCGGCGGGCAGTAAGCTGCAGCCTGGCGCGG GGCACCGGAGCCCCGAAGTGCGGGAGTGACTCGACTCCGCCTCCGCCTCTACCCCGCCGC AGGAGCCGTGCGCGCCGCGGCCATCTTGGAAACGGCGACCGGCCGAGCGCCGGGGCTACG GTGGGCGGCGAGGGACAAGCCTCCCAGATCGGCGGCGGTGGCGGCGGCGGCGGCGGGCGC CGGAATGCGACCGGACGGGGGCGGAGACGGCGGCAGGGAGGGGCGGGGAGCGCCGTCAAG TGTCGGCGCGATTCGGTCCTGGAGCCGGGTCTCGCCAGCTCGCCCGGTGTCGCCCCGGCC GGTGGCAGTGGGGGACTGTGGAGCGGAGCCGGGCTGTGCAGCGGCCTGGGAGCTCGAGGC TTCCCAGGCGGTCGTCGGTCTGGGAGGGACGCTGGGTAG Clone variation with respect to AK094629.1 465 g=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094629 unedited GGGGTCAGAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCGTTGA GGGTGAGCTGGGGGAGTCTACAAGGGAAGGGGAGCCGGCAACACGCTCGAGCGGAACCCT GCGGAGCTGCAACCCCGGGAGGAGTCGCCGGACAGACTCAGACGCGGTCCCCTGGGACCC AGGGATGCACCGCGCCCCTCCCGCCCCAGCCTGCAGAGACTGGATGCTTCCCTCCCCACC TGGCGGGAAGCAGAGGCCCTCGGGCCCGACCGAGTTCGGGCCAGGACCCGCCCCGCTTTC TGCGAGCGACCGACTGCGGCCGCCACACTCACCTCGCCGCCAAGCAGCACTCGGGTTCTG ACTCGGTCTCGGGTTCCGGTGGCGGTGGTGGCGGCGGCGGCGGTGGCGGCGGCTCCTCCA TGGCGGCGGCAGGCGTGACGGCAAAGGCGGGAGGAGGGACCAGCGCGGCAGCGGCTTCTC TCATCCGTGCACGGAGCCCCGCATGGCCGCGGCGGGCAGTAAGCTGCAGCCTGGCGCGGG GCACCGGAGCCCCGAAGTGCGGGAGTGACTCGACTCCGCCTCCGCCTCTACCCCGCCGCA GGAGCCGTGCGCGCCGCGGCCATCTTGGAAACGGCGACCGGCCGAGCGCCGGGGCTACGG TGGGCGGCGAGGGACAAAGCCTCCCAGATCGCGGCGGTGGCGGCGGCGGCNGCGGGCGCC GGAATGCGACCGGACGGNGGCNGANAACGCNGCANGGANGGGGCGGNGAAGCGCCNTCAA GTGTCGGCGCGATTCGGTCCTGGACCCGGTCTCGNCAGCTCGNCCGGTGTCNCCCNGNCC GTGGCANTGGGGACTGTGGAGCNNGACGGGCTGTGCANCGGCCTGGGAGCTCNAGCTTTC CCAGCGTCTTCGTCCTGGAGGGAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094629 unedited GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAGAT TTATTTTATTATTTAACTTTTAAAGTCTCTAATATGCCTTCTAAGAAGGGTTCTGGCTCA GAAAATTGCAGAAGAAAATCGAGTAGAGAACTAGAATTACAGAAACCTAAGAAATACTTT TACTGAGCTTTCTTCACACAAGAAAATGCACGGAAATGAATTATACATGGATACCAATTT GTGATTTTTAAAAATTTGTAATTTTTTAAATTAAGATAAAAATTAGTTTATTTTTTTGAA TACTTTTTTGTTTTGTTTTACCTCACCCTTTACTAAGCTGTTAGCTTGGTCTGTTGCGCT TACTGCTATATCCTCAGCATCTAGTACAGTTCCTGACACATATATGCACTTAATAAGTAT TTGTCAAATGAATATTATTGAGTAAATAAGATGAAGGTCAATTCAGAAATTCAGCACTAT CAGGCTATATACATAATACAGACTATTATAAAAAACTAGAATGGAGCAGTTCCTTTTAAC GACGGAGAAATGTCAGTAACATACTTTGAGCACAATATAGCTAATTTCACAACTGGTCTT TAAATATAACATACCTATAATCAATTTAATGTTTTTCTTTGTTACCAGAGTTGTTCAAAA CGTGCGTTTTACCTATCAAAGCTCAACTAAATGAGGTTTAAGAAATCTGCACATTTGAAT GTACTTAATTTTAAAAGATTCTGGTTTTGGATTAACTCTGGATTTCACCCTATGGGAAAT TTTGTAATATTTCCAACTTAAAAAAACTGTGAAGCCCAGCGCTAGAGTAACGATGCATTG GATTTCACAGGATTCTGTACTCTGAATATCCTAGCATTTCTCTTCCCCATCCCTCGGGTA GAGCATCAGTCCAGTTCTTGGAATCGTTCGATCTAGACCTGGTGAGAGTTTGCGGNTGAA TGAGGGTCCTTAAATCGGATCCGAACTTTGTCGCTAAA
Restriction Sites	NotI-NotI
ACCN	AK094629
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK094629.1, BAC04390.1
RefSeq Size	2028 bp
RefSeq ORF	2028 bp
Locus ID	4216
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	GnRH signaling pathway, MAPK signaling pathway
Gene Summary	The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014]

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