DNA polymerase mu (POLM) (AK092801) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Pol Mu; Tdt-N
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092801, the custom clone sequence may differ by one or more nucleotides GGGGTCTTGGTAATGTTGCCTAAGCTGGTCTCAAACCCCTATGCTCAAATGATTCTCCTGCCTTGGTCCC CAAAATGCTGGGATTATAGGCATGAGCCACTTCGCTCTGCCTTGACTGTTTTTGATGGAATATTCTCAAG TAGATTACATGCTGTGGCATCTTAAACAGAGCACTGTGAACCAAATGTACCCTTGGGTGCTGCCACTGAG CATGCTGGGCTTGCCCTTGCCCGCCTTCTGCAGGACCTTATGTGTTATTTTCTCAGTGTTGTCAGCCTGC CTGTTTCCATGGCCACCTTCCCTCTGTTTCTGCTCACTCCTGGTCTGATAGGGAAATGCCGTCTGAGTTC TTTTGGATGCCTCGGGTGTGCCTGCTTTGACACCCTTCTGCCAACTGGCTTTGTGGCTGGAGGTGTCACA GCGGTGGCCACATGGATAACTGTCAGTGAAGTGTGGCTTCCTGGGTGGATGGGGCCTTGGGAGGTCACAC AGAATGTTGTCAGGATTGACAGGGAGGGCTTCGAAGAGGAGAGACTTGAGCAGTGTGTGCAGGGCAAATG GGCTCAAATATGCAGGGAAGGGGGCAAGGGGCACTGGCAGGGACAGGGGACCAGATGAGTGGGGCACCGT GGTGAGTGTGGGAGTGAGGGGCCTGAAGATGATGAAATCCCCTTGAAGTGGTGTGAGGTTAGTGAGTACT TTCACTGTCAGCCTCAGCCCCAGCCCTGAAGGCATCTAGGCTGGCAGAAAATTACATTAGTAATGAGAAG TTTATTAACAATATAATTGGGTTAATCTCATCATTTTTAGTCAGGATTCTTTGGGTTGCAAGTGACAGTG ACCCACCCCACACCGTTGGTTCACAAAATAAAGGAAAGGAGGGCCAGCAGAGCCCAGGATGGGGAGGGCT GTGGCCGGTCTGCAGGACAACTGGGCTCAGCGACCTGGGTATCACCAGGCCACTCCTCCTCCCCTCTGCC CCTCTGCACGGTGGCTTCGCCCTCTCTTGCGACGCACCAGCTTCTTCCATCGGTTGGAAATGTGGCCACA AAAGGTGCTCGGCTGCCACAGGCTACAGCTTCTGCCACTGTGTGGGCCTGCCCTCAGTCTCAGATCCAGA ATTCCTAGGGAGGGACTCTAGTGGCTGACCTGAAGCAGGTGACTTGCTTGGGCTGGGGCCCACCCTCTGT GGGAAGATATGTGAGCACCCCAGCCAGCCCTGCCAACTCTGCTCCTAAATCCACCCTTTTGATTGTTAAC TCCTCATTGTTTTTTGCTGTTGGCTAAGGAGGAGAACCACAGAAATTTGTTCCACCTTCTGTTAGGTGGG AGAGGCATGGTTTTTCCAGTGACTTAGGAGCCCCTCGTGCTGATGTGTTAGGGTGACTGTTTTGATTATG TTGGAATCACAGGCTAGAGCTGTGTAAGTCCCTTTCATAGAGGAGAGGAGAGGGGTGTGCGTGGGTTTGT TCCCAGAGACGGCAGGCTGGGTAGTCAGGCTCCAGGTTTGAATCCTTGCCCTGTGGTCTACGGCAGGTTG CGAGTGGGGACGGCGCCTGCCTGGTGCATGGTGCTCCATGGGCCACACCGTCCCGGAGCGGGTGATCATC CGAGTCTAGCCATGAGAGCTCTGCCCAACCGGGCTACGCTCCTTCCCCACAGCTCTTCACCCAGATCTTC GGGGTCGGTGTGAAGACTGCTGACCGGTGGTACCGGGAAGGACTGCGAACCTTAGATGACCTCCGAGAGC AGCCCCAGAAACTAACCCAACAGCAGAAAGCGGGTGAGCCCTCCAGAGAGGCTGGGCCCTGGGCTTCCCT AAACTGCACCCTGGATCCCTCAGCATCAACTCCCTAGAGGGAAGGGAGCAGTCTGGTGCAGAGGGTTTGG TGGCAGCTTTGTGTGGTGGGCTTCCTGGGGTCTCCACCCAGTGCAGTCAGGATCTATCAAAGCCTTTTCT CAGTGGACGATGCTGACCACAGCAACTTCCATGATATGCCAGGGGCTTGTGTCTGCCTACTGGTTAGACT TTGACCAGTGACCAGGGACAGCCTGGGCCTTGGGCACACTGCCCAGTGTGGTGCTTGGTACATGGAGGCC TGCAGAGGTGGCACTGAGCAGCAGAAAGAACATCAGCTTTCAAGTTTGTTAGGCCTGGGTTTGAGTCTTA TCTACCATTTGCCAGGTAGGTGACCATTGCCCAAGAAATAACCTCTCTGAGTCTGTTACATTTTCTGTAA AATGGAGAAGGAAGGCTGGGCATGGTGACTCACACCTGTAATCCCAGCACTTTGGGAGGTCAAGGTGGGT AGATCGCTTGAGCTCAGCAGTTCAAGACCAGTCTGGGCAACAGGGTGAAACCCTGTCTCTACGAGAAATA CAAGAGTTAGCCAGGCGTGATGGTGGATGCCCGTAGTACCAGCTACTCATGAGGCCGAGGTACAAGGATC GCTCGAGCCCGGGAGGTCAAGCCTTGAGGCCAAGGTGGAAGTTGCAATGAGCTGAGATTGAGCCACTGCA CTCCAGCCTGAGTGACAGAGCAAGACCCTATCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092801 unedited TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCAGTCTCTGGAG TAGCTGGGACTACAGGCATGTGCCACCACACCAGGTTAATTTTTAAATCTTTCTCGTAAA GATGGGGTCTTGGTAATGTTGCCTAAGCTGGTCTTAAACCCCTATGCTCAAATGATTCTC CTGCCTTGGCCCCCAAAATGCTGGGATTATAGGCATGAGCCACTTCGCTCTGCCTTGACT GTTTTTGATGGAATATTCTCAAGTAGATTACATGCTGTGGCATCTTAAACAGAGCACTGT GAACCAAATGTACCCTTGGGTGCTGCCACTGAGCATGCTGGGCTTGCCCTTGCCCGCCTT CTGCAGGACCTTATGTGTTATTTTCTCAGTGTTGTCAGCCTGCCTGTTTCCATGGCCACC TTCCCTCTGTTTCTGCTCACTCCTGGTCTGATAGGGAAATGCCGTCTGAGTTCTTTTGGA TGCCTCGGGTGTGCCTGCTTTGACACCCTTCTGCCAACTGGCTTTGTGGCTGGAGGTGTC ACAGCGGTGGCCACATGGATAACTGTCAGTGAAGTGTGGCTTCCTGGGTGGATGGNGCCT TGNGAGGTCACACAGAATGTTGTCAGGATTGACAGGGAGGGCTTCNAAGAGAGAGACTTG AGCAGTGTGTGCAGGGCANATGGGCTCAATATGCAGGNAAGGGGGCAAGGNGCACTGGCA GGGACAGGNGACCANNATGAGTGGGCCACCGTGGTGAGTGTGGGAAATGGAAGGGCCCTG AAAAATGATGAAATCCCTTGAGNTGGGGGAAGGTTAANGAGTACTTTNACTGTCAGCCTC AGCCCCAGNCCTGAGGCATNNTAGCTGGCAAANATACATTAGAAGGGGAGAGGTTATTAC ACTTTGATTGGGGTAATCCATATTCNTAGCAGGATTCTTTGGGGTGGCAGGGAAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092801 unedited GACTCTAAGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTGAGAAAGGG TCTTGCTCTGTCACTCAGGCTGGAGTGCAGTGGCTCAATCTCAGCTCATTGCAACATCCA CCTTGGCCTCAAGGCTTGACCTCCCGGGCTCGAGCGATCCTTGTACCTCGGCCTCATGAG TAGCTGGTACTACGGGCATCCACCATCACGCCTGGCTAACTCTTGTATTTCTCGTAGAGA CAGGGTTTCACCCTGTTGCCCAGACTGGTCTTGAACTGCTGAGCTCAAGCGATCTACCCA CCTTGACCTCCCAAAGTGCTGGGATTACAGGTGTGAGTCACCATGCCCAGCCTTCCTTCT CCATTTTACAGAAAATGTAACAGACTCAGAGAGGTTATTTCTTGGGCAATGGTCACCTAC CTGGCAAATGGTAGATAAGACTCAAACCCAGGCCTAACAAACTTGAAAGCTGATGTTCTT TCTGCTGCTCAGTGCCACCTCTGCAGGCCTCCATGTACCAAGCACCACATTGGGCAGTGT GCCCAAGGCCCAGGCTGTCCCTGGTCACTGGTCAAAGTCATACCAGTAGGCAGACACAAG CCCCTGGCATATCATGGAAGTTGCTGTGGTCAGCATCGTCCACTGAGAAAAGGCTTTGAT AGATCCTGACTGCACTGGGTGGAGACCCCAGGAAGCCCACCACACAAAGCTGCCACCAAA CCCTCTGCACACGACTGCTCCCTTCCCTCTAGGGAGTTGATGCTGAGGGATCCAGGGTGC AGTTTAATGAAGCCACGGCCCAACCTCTCTGGAGGGCTCACCCGCTTTCTGCTGTTGGGT TAGATTCTGGGGCTGCTCTCGGGAGTCATCTAAAGGTCGCAGTCCTTCCCGGTCCAACGT TCAGCATTCTTACACCGACCG
Restriction Sites	NotI-NotI
ACCN	AK092801
Insert Size	2800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK092801.1
RefSeq Size	2554 bp
RefSeq ORF	2554 bp
Locus ID	27434
Protein Families	Druggable Genome
Protein Pathways	Non-homologous end-joining
Gene Summary	Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.[UniProtKB/Swiss-Prot Function]

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