RPL14 (D87735) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAG-ISL-7; CTG-B33; hRL14; L14; RL14
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102367 sequence for D87735 edited (data generated by NextGen Sequencing) ATGGTGTTCAGGCGCTTCGTGGAGGTTGGCCGGGTGGCCTATGTCTCCTTTGGACCTCAT GCCGGAAAATTGGTCGCGATTGTAGATGTTATTGATCAGAACAGGGCTTTGGTCGATGGA CCTTGCACTCAAGTGAGGAGACAGGCCATGCCTTTCAAGTGCATGCAGCTCACTGATTTC ATCCTCAAGTTTCCGCACAGTGCCCACCAGAAGTATGTCCGACAAGCCTGGCAGAAGGCA GACATCAATACAAAATGGGCAGCCACACGATGGGCCAAGAAGATTGAAGCCAGAGAAAGG AAAGCCAAGATGACAGATTTTGATCGTTTTAAAGTTATGAAGGCAAAGAAAATGAGGAAC AGAATAATCAAGAATGAAGTTAAGAAGCTTCAAAAGGCAGCTCTCCTGAAAGCTTCTCCC AAAAAAGCACCTGGTACTAAGGGTACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCT GCTGCTGCTGCTAAAGTTCCAGCAAAAAAGATCACCGCCGCGAGTAAAAAGGCTCCAGCC CAGAAGGTTCCTGCCCAGAAAGCCACAGGCCAGAAAGCAGCGCCTGCTCCAAAAGCTCAG AAGGGTCAAAAAGCTCCAGCCCAGAAAGCACCTGCTCCAAAGGCATCTGGCAAGAAAGCA TAA Clone variation with respect to D87735.1 194 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for D87735 unedited AAGGAGGTTGANATTTGTNATACCACTTATATAGGCGGCCGCGNAATTCGCACCANAGGT GAGTCTTACTGTTGCGGGCTCCGGGGCCGTCGACCATGCCGCTCGACCTCCACCTCCGCT GGGAAGCTGAGGCGCCGAACGGCTCCCAGNAGGGTCCCGGGNAAGCGCATGGTGTTCAGG CGCTTCGTGGAGGTTGGCCGGGTGGCCTATGTCTCCTTTGGACCTCATGCCGGAAAATTG GTCGCGATTGTAGATGTTATTGATCAGAACAGGGCTTTGGTCGATGGACCTTGCACTCAA GTGAGGAGACAGGCCATGCCTTTCAAGTGCATGCAGCTCACTGATTTCATCCTCAAGTTT CCGCACAGTGCCCACCAGAAGTATGTCCGACAAGCCTGGCAGAAGGCAGACATCAATACA AAATGGGCAGCCACACGATGGGCCAAGAAGATTGAAGCCAGAGAAAGGAAAGCCAAGATG ACAGATTTTGATCGTTTTAAAGTTATGAAGGCAAAGAAAATGAGGAACAGAATAATCAAG AATGAAGTTAAGAAGCTTCAAAAGGCAGCTCTCCTGAAAGCTTCTCCCAAAAAAGCACCT GGTACTAAGGGTACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCT AAAGTTCCAGCAAAAAAGATCACCGCCGCGAGTAAAAAGGCTCCAGCCCAGAAGGTTCCT GCCCAGAAAGCCACAGGCCAGAAAGCAGCGCCTGCTCCAAAAGCTCAGAAGGGTCAAAAA GCTCCAGCCCAGAAAGCACCTGCTCCAAAGGCATCTGGCAAGAAAGCATAAGTGGCAATC ATNAAAAAGTATAAAGGTTCTTTTTGACCCTGTAAAAAAAAAAAAAAAACTCGACTCTAG ATGCGGCCGCGTCATAGCTGTTT
Restriction Sites	NotI-NotI
ACCN	D87735
Insert Size	900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	D87735.1, BAA13443.1
RefSeq Size	722 bp
RefSeq ORF	663 bp
Locus ID	9045
Domains	Ribosomal_L14e, KOW
Protein Pathways	Ribosome
Gene Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

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