VPS36 (AK022911) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C13orf9; CGI-145; EAP45
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK022911, the custom clone sequence may differ by one or more nucleotides TAGACATGCCCAGAGTTATGATTACAAATTTAGGAGGTAGACGGCTCAGGAATTCCCTGGGATTGTTGTG CTGGTGGAATGGCAGAGGGAACTTCACAGGAACCTTAGTGCTCTTTTACCTCAAAGCCACAGACAGGAAA TAGAAAGTGGAAAAGTAATATCTCCTTTTCTTTTCCATAAGGAGTTTCAACACTGAACTTTAAAAAGTCT ATCATATTCCAGCAATATTTTTTCTTTGTCCTTTATGTTGTAAGTTGTGTGGAAAAACTACTTCGGTAAG AAATGTTACTGAGATAACAACAACTGGCTAATACTGCATGTAGATTGCTTAGGTTTTAAAGTGACTGCCT GACTTCACATGTTATTGCTACAGCCTCCAGTATGTTCGCATTATCTCAAACTCAGGGACCCCACAGGACA GGAGACACCCTTTCTGAAACTGAGTTGGAAGTGAAAGGGTGGTGATGGTTTTGGCCAAGCCTGCGGGAGG GAAAGTATTGTATTGGGAGCACCCTTGGGACCAGGAAGAGGGATGCCCAGGTTCACACTCTGGGACCCCT AAGACATTGTCAGTGGGTAAGGTGGAGGGCCACTGCCAGAGGCTGCTGCAGTGCCCTCTGGGAAGTGCCA GGGACCCTCAGCTGCAGACCCAGGGGATCCCAATCCCTTATCTCTGCCTGAAAAGTGGCTATTGCTGCTT CGTATCCTCCAGTGTCACAGGAATGCTGTGCCTGAGACATCCCACTTTAGTTTTTGTTTTCCAAACCTGT CACTGACTCTTCATTGTGGTGACAGATCCTCTGGTTCCCCCATTGCTCAGCCTCAGGTTTATTTTGAGGG TAACTCAGTGTCTGACTGCCCAAGTCTTTTAGGGGTTAGTTGGACCATTGTGTAAGCTTGTTTGTATGTC TCACCTCTTTCTGGTTGGTACTTACTGTCTCACACTGCTCTGGCAAACTTGTACACACACACACACTCTC TCTCTCTCTCACCTGGCTAAGGCTTTTAAAATTATGAAGATAAATAATCTGTTTCACCAGCTGGAGAGAG TTGCAAGGAAGATTGCTGGAGCTACTCAATCTAGCGCTAATGGTTTGGATTCATTACTGCAAACCTACAT AATTTAACATATTTGTTTACTTTAGTGTGACAACTGATGAAAAAAAATGGAGCAATCTGAATTGTATAAA ATAACTTAAGAAGGAAGAAAAGTGATATATAAATATATTTTGCAAATGTCACATTAATTTAAAAATGAGT ATGATTGATTTTATTTTTAAAGTGGGCATTCTTCACTGTTTCGAGACCTTTGTATGTATTTGTGTATTTT TATCTTTTTTTTTCAGGCCATTATTATAAGGTGTTATTTTGGCCCTCTAATGTAGAAGTTATGTTTAAAT ACAACCAATCAGGCCCTAAGTGCAAAAAAGTTCTGTCTTGGTGCTTATCACTGGTATAATTATTATTTTT TTGTTTTCCTAACTTTGCTCTTAGGAGCATGAGCCTCTTTGTAGCTTTATGGTAATGCAATATTTCTGGT CATTTACTGTCAAAAAATTTTTTTACATTGTGTTAGTGAAGACTGTGTTTTCAGGGTTAAATGATTGGTA TCAATGTATATAGAGTAAAATTATTGCAAAATTTAAAAATGATTTTTCTTGGCATTCTTTTTAAATACTC TGAAACATATATGCAAATGAGAAACCTTTAATCATACTAAAGCCAGTTATGTTAAGAATTTTTCCTTTGG ATTTTATAATTAAGAGTTGCTTATAAATACTTATGACATTGAGCTCTTACTGTTTTAGTTGTCTTAAAAG AATGGGACATGTCATGAACTGGTTTCTTTTTTTAAAAAATTCTTGATGTGAATTCCACTGTTACCTGACC ATGTATATTCAGATGAATGTACTCTTTCTTGTTCCTTGTTACCAGATTAATCTGAGAGAATGTGGATTCA TCCTGTCATCTTTCTCATGCTCAAATACTTAAAATGGTTCTTTTGCCCAAAAGTCTCAGAGTCTTCATTT TGGTACAGTGGAGTCACAACTTGTAAGGTGGTTAGGTTTTAAAGACCGTGCCTAAGGACAAAATTGAGCA TGCTCAGAATGGTCCTTGAAAATCTCCTAAATTACCAATAAATTTCTCTTTGTAGTCTTAAAGTTTAAAT CCCTGTGGAGTGATATGTAGGTATGAATATATTATGCTTATATTAAGTACAAAAATACAAACTGCATATC AAGAGATTCTTATAGCATTAATAATTTCCATGCATGTGTCTTTTTCCAGTAGGTATGGTTGAATTTATGT AAATTTATTGCTAATCCCATCCCTTACGATCTAGAGTATAAGCTGCGCAAGGGCAGAAGTTTTTATCTGG TTTGTTCATGGATGTATTCTAAGAGCTGAGAACAGGGCCTGGACACAATAAGCATTCAATAAATATTTAC TGAATGAATG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK022911 unedited AGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGAGTTTTTT TTTTTTTTGTTTCCAAATGCTGTGGATTTTGAAACTGAACTAGAGAGCTGTATAGACATG CCCAGAGTTATGATTACAAATTTAGGAGGTAGACGGCTCAGGAATTCCCTGGGATTGTTG TGCTGGTGGAATGGCAGAGGGAACTTCACAGGAACCTTAGTGTTCTTTTACCTCAAAGCC ACAGACAGGAAATAGAAAGTGGAAAAGTAATATCTCCTTTTCTTTTCCATAAGGAGTTTC AACACTGAACTTTAAAAAATCTATCATATTCCAGCAATATTTTTTCTTTGTCCTTTATGT TGTAAGTTGTGTGGAAAAACTACTTCGGTAAGAAATGTTACTGAGATAACAACAACTGGC TAATACTGCATGTAGATTGCTTAGGTTTTAAAGTGACTGCCTGACTTCACATGTTATTGC TACAGCCTCCAGTATGTTCGCATTATCTCAAACTCAGGGACCCCACAGGACAGGAGACAC CCTTTCTGAAACTGAGTTGGAAGTGAAAGGGTGGTGATGGTTTTGGCCAAGCCTGCGGGA GGGAAAGTATTGTATTGGGAGCACCCTTGGGACCAGGAAGAGGGATGCCCAGGTTCACAC TCTGGGACCCCTAAGACATTGTCAGTGGGTAAGGTGGAGGGCCACTGCCAGAGGCTGCTG CAGTGCCCTCTGGGAAGTGCCACCGACCCTCATCTGCTNGCCACCCAGAGGGATCCCAAT CCCTTATCTCTGCCTGAAAAGTGGCTATTGCTGCTTCGTATCCTNCAGTGTCACAGGAAT GCTGTGCCTGAGACACCCACTTTAGNTNNTGNTTTNCAAACTGTCACTGACTCTTCATTT GTGTGACGATCTCTGNTCCCATGCTAGCTAGNTATTTGAGTACTCATGCTACTGCATCTT TAGGTANTGACATGGAAACTGTGAGCTACTCTTTGTGACTCTGCTACTGTTGCACTGACA CCAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK022911 unedited AAATAGCTTGNACGCGGCCGCTTTTNANGATCGAGTTTTTTTTTTTTTTTTTTATGTGTT CAAAAAAATGTTTTTTTTATTGAATTGAATGGGAGCTAAAGTAGGATAAAGTGGAGCCAA ATTATAAATAGGAATATAGGTAGGAGTTCATTCATTCAGTAAATATTTATTGAATGCTTA TTGTGTCCAGGCCCTGTTCTCAGCTCTTAGAATACATCCATGAACAAACCAGATAAAAAC TTCTGCCCTTGCGCAGCTTATACTCTAGATCGTAAGGGATGGGATTAGCAATAAATTTAC ATAAATTCAACCATACCTACTGGAAAAAGACACATGCATGGAAATTATTAATGCTATAAG AATCTCTTGATATGCAGTTTGTATTTTTGTACTTAATATAAGCATAATATATTCATACCT ACATATCACTCCACAGGGATTTAAACTTTAAGACTACAAAGAGAAATTTATTGGTAATTT AGGAGATTTTCAAGGACCATTCTGAGCATGCTCAATTTTGTCCTTAGGCACGGTCTTTAA AACCTAACCACCTTACAAGTTGTGACTCCACTGTACCAAAATGAAGACTCTGAGACTTTT GGGCAAAAGAACCATTTTAAGTATTTGAGTATGAGAAAGATGACAGGATGAATCCACATT CTCTCAGATTAATCTGGTAACAAGGAACAAGAAAGAGTACATTCATCTGAATATACATGG TCAGGTAACAGTGGAATTCACATCAAGAATTTTTTAAAAAAAGAAACCAGTTCATGACAT GTCCCATTCTTTTAAGACAACTAANACAGTAAGAGCTCAATGTCATAAGTATTTATAAGC AACTCTTAATTATNAAATCCAAAGGAAAAATTCTTAAACATACTGGNCTTAGTATGATTA AAGGTTTCTCATTTGCATATATGTTTTCAAN
Restriction Sites	NotI-NotI
ACCN	AK022911
Insert Size	2400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK022911.1
RefSeq Size	2460 bp
RefSeq ORF	2460 bp
Locus ID	51028
Protein Families	Transcription Factors
Protein Pathways	Endocytosis
Gene Summary	This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013]

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