RPS6KA3 (AK096084) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096084, the custom clone sequence may differ by one or more nucleotides CAAATTAGCCAGGTGTGGTGGCTGGCACCTGTGTTCCAACTACTTGGGAGACTCATGTGACAGGAAGATC ACTTGAGCCCAGGAGTTAGAAGCTGCAGTGAGCCGTGATCTTGCCACTGCACTCCAGTCTGGGCAACACA GCAAGATACTGTGTCAAAAAATTTTTTTTTGATAAAAAATAAAAGAGTTACATGACATTCAGAGACCATC CAAAAAACCTGTGGGTGCCCGGCTGGGCTCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAA AGTGGGTGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGACAACATGGTGAAACCCCATCTCTACT GAAAATAGAAAAAATTAGCCAGGCATGGTGGTGGATGCCTGTAATCGCAGCTACTCGGGAGAGGGCGCTG GAGAATCACTTGAACTCATGGTGCGCAGGTTGCAGGGAGCCAAGATGGCACCATTGTGCTCCAGCCTGGG CAACAAGAGCAAAACTCCATCTCAAAAAAAATAAAGAACCTGCGAGTGAGTTCCCACACGTTTTCCTAAT GGGCTGCTGCTTTCCTAGGAGTCTCTCGCTCATAGAAAAGGCACAAACTGAAAGAGGAAGCAGATCCCAT TGCTGTGGAAGTCCCATTGTTAGGAAGCTCTGCTTTTCTGGAGTTCAAATTCGCATTCATGACGCTTTAA ACCGTCAGAGCTGGGTGGGTCCTCCTACAACAAAATAGTTTGCTCTCTCTCTCCTAGTTAACAGGCTTTC AAATATTAGAAGATCAATGTTCTGACCCCATTAAAATTTCTCTTTTGTGGAATGAAAAGCTCTGATTTAA CCCATCTTCAAGGCTGGTTTGATGGAGGAATAGGGGCTGAGTCACCTGCATTTCCCCTCCCTGCACAAAG TCCTGGGCCCAGATCTGGGGTCTGTCTCTGCTGAGGGTGGGGTGAACCAGGAAGCACCTCCTTCTTCATC TCCTTGATGAATGGGTATAATGGTTGCCATGGAACTGGGGCTTGTTTGATGACCTGGGGCTGGGTGGGCC TCTGAGAGCCTTTATAGCTGATTGCCTTTTGGGAGAGGGGAGGTGGGAGCCCCACCCTGTCTCATGAGTC ACCCCAAAGGTGCATGGGCAGGCAGGTGCTGGGGAATCGGCTACTCCCCAGAGCTTGGCGTGGCCATCCC TGTGGCCCCTCTGGGAGTCTGGAGCCCATTCCCTCACGCTGGTACTCACTGCAGCTGGGGACATCTGCAC TAGGAAGACAGGACACGGCATGGAAGCTGGCCTCTGCCCAGAAGCCATGACATTCTGGTCACCAGCCTGA TGCTATAAAACGAGTGTCACGGCCGGGCATGGTGGTTCACACCTGTAATCCCAGCACTTTAGGAGGCCAA GGCGGGTGGATCATGAGGTCTGGAGTTCGAGACCAGCCTGGCCAACATGGCGAAATCCCGTCTCTACTAA AAATAAGAACATTAGCCAGGTGTGGTGGCACATACCTGTAGTCCCAGCTCCTCTGGAGGCTGAGGCAGGA GAATCACTTAAACCCAGGAGGCGGAGATTGCAGTGAGCCGAGACCACGGCATTGGACTCCAGGCTGGGCA ACAGAGCACGACTCCATCTCAAAAACAAACAAAAAAAAAGAGTGTCACCTGGGGCTACTTGGCCAGACAC AGAGAGCAAGGAGACATCCCTATTATCTGTCAAAAATAATTGTTGGGGCTGAGCACAGTGGCTCATGCCT GTAATCTCAGCACTTTGGGAGGTCGGGGCAGGAGGACTTGAGGCCTAGAGTTTGAGACCAGCCTGGGCAA CATAGCGAGCACCCCATCTCCAGAAAAAATTTAAAAATTGGCTGGGCGCAGTGGCTCATGCCTGTGATCC CAGCACTTTGGGAGGCCGAGGGGGATGGATCATTTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATG GTGAAGCCCCATCTCTACTAAAAATACAAAGATTGGCCGGGCATGGTGGTGGGCACCTGTGGTCCTAGCT ACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGCGGTGGTTGTAGTGAGCTAGGATCATGC CATTGCAGTCCAGCCTGGACAGCAAAGCTAGACTCCATCTCAAAAAAAAAAGAAAAGAAAAAGTAAAAAA TTTAAAAATTAGATGGGCATGGTGACATGTGCCTGTAGTCCCAGGTACTAAGGAGGCTGAGGTAGGAGGA TGACTTGAGCCTAGGAGTTCGAGGCTGCAGTGAGCTCTGATCGCACCACTGCACTCCAGCCTGAGTGACA CAGCGAGACCCTGCTTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096084 unedited TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGTGGCTGGCA CCTGTGTTCCAACTACTTGGGAGACTCATGTGACAGGAAGATCACTTGAGCCCAGGAGTT AGAAGCTGCAGTGAGCCGTGATCTTGCCACTGCACTCCAGTCTGGGCAACACAGCAAGAT ACTGTGTCAAAAAAAATTTTTTTGATAAAAAATAAAAGAGTTACATGACATTCAGAGACC ATCCAAAAAACCTGTGGGTTCCCGGCTGGGCTCAGTGGCTCATGCCTGTAATCCCAGCAC TTTGGGAGGCCAAAGTGGGTGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGACAA CATGGTGAAACCCCATCTCTACTAAAAATAGAAAAAATTAGCCAGGCATGGTGGTGGATG CCTGTAATCGCAGCTACTCAGGAGAGGGCGCTGGAGAATCACTTGAACTCATGGTGCGCA GGTTGCAGGGAGCCAAGATGGCACCATTGTGCTCCAGCCTGNGCAACAAGAGCAAAACTC CATCTCAAAAAAAATAAAGAACCTGCGAGTGAGTTCCCACACGTTTTCCTAATGGGCTGC TGCTTTCCTAGGAGTCTCTCGCTCATAGAAAAGGCACAAACTGAAAGAGGAAGCAGATCC CATTGCTGTGGAAGTCCCATTGTTAGGAAGCTCTGCTTTTCTGGAGTTCAAATTCGCATT CATGACGCTNTANACCGTCAGAGCTGGGTGGGGTCCTNCTACACAAAATAGTTTGCTCTC TCTCTNNCTAGTAACANNGCTTTCAAATATTAGAAAGATCATGNTNCTGACCCCATTNAA AATTCTCTTTTGTGGAATGAAAGCTCTGATTTAACCATC
Restriction Sites	NotI-NotI
ACCN	AK096084
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK096084.1
RefSeq Size	2327 bp
RefSeq ORF	2327 bp
Locus ID	6197
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
Gene Summary	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]

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