PSMD13 (AK092845) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSPC027; p40.5; Rpn9; S11
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092845, the custom clone sequence may differ by one or more nucleotides GTCTTTCTATACTTTACAGCCTGATTTAGCAGCTAATGAAGCCCAGCTTCTGAGGAAAATTCAGTTGTTG TGCCTCATGGAGGTAAGCGAACACCCAGGAGCCACTTTGTCTGTGGTTTCAGATTTTGGTCAGGTGGGGG CGCTGCACTCTCCAAGCCTGTGCTGAGCAGTGTGAGCTTTCAGTGGTGCTGCTTCTGCTGTGCGCCGCTC TCTTCACCACCTTCCTTTTCCTTTGCTACCACTTGCTCTTTTAAGTTTATATTTGGCTCTTAGCTCAGAC GACACCCTCTCACCCCACTGCCACCACCCTGAGGCCTTCCCTGACCATCTGAACTGAGATGAAGTAGCTG CCCCTCTCCCCGTGATGCAGTTGTTGCCTCATTGCATGTCGCTTCTTGTGTATACGCACATCTGTCTTTC CCCACTAGAACTTAAGCTTCGTCAGGAAGTGGCCCTTAGATTTCTGGTTTGCCGTGGTCACCCCTGGTCA ACCCTGGCAAATTGTGGCCTCAAGTACTTGTTCGGGGATTGAACAATGGCTACTGTCAGAAACTCCTGTA AGGCATTTTGCTGTTATCCTTCTTATCTAAGCATTAAAAGCTTGTTCTTAACAAGATATATGTCTAATAT TAGGAAACTTTTTAGTATGTGGGGTACTAATAAGATCTCTATTTTCAGAGCCAATATTGACAAAACATCC TTATCAGTTCTCTATTTTTATTTGGAAAAATAGTTTAAAATAGTTAATAAAATTTTTTCCAGATGACTTT CACACGGCCTGCCAATCACAGACAACTCACTTTTGAAGAAATTGCCAAAAGTGCTAAAATCACAGTGAAT GAGGTACGGTCCCTAGGCTCAGGGTGTTAGAGCAGCGAAGCTGCCACATGGAGGAGTCAAGGCTCTTGTG TGAGCGCTGTGCTCCCTAGACAGTAAAAAATGACGGGAGGAGCGGCATCTGCTTCTCACAAGCCATCTGG GTCCATGGGGGTGTGTCAGGCTATCTTGTCTTACACACGCCTCCCTCTCTGCACAGGTGGAGCTTCTGGT GATGAAGGCCCTTTCGGTGGGGCTGGTGAAAGGCAGTATAGACGAGGTGGACAAACGAGTCCACATGACC TGGGTGCAGCCCCGAGTGTTGGATTTGCAACAGGTGATGTGTTTGAAACCCATTATTCACCTCTGTGGAT TTTGAGGGGTTGTGTCTATACCGTCTTAGTTTCATTTGGATGGAAGCCATTTGGGAAGACAGCATTATTA GACAAGAGGTTTTGGAGAAGGAAATACTGCTGTTGGGTTTTTCTAAGAGCCTAATTTAATGCAAGCCTAG GGTTTGAACCCTGCATTTAAAAGCTTATGATGACAATGGCACTGGTTGTGCTGACGGTGCCTTTTAATCT TAAGATAGGAGCTCTGATCAGATACGTTCCTGGTTCTGTGATTTGAGCTCACGTCGCTCTTACATTTGCT GGAAATGCGATCCTGTGGATTTCCTCTGTCCTTGTCTGCTTTCTTTCATGCAAGTTTTTTCTAACGTTCC TGTGAAAAGAATTAACCCGGCAAGTCCCGTCCCACTCCCCCAGCTCAGAGGTCCTTTTTACTAGAGCTGC CCTAGAGAGTTAGCTGGAGATGTAGAGTCACCCCATCAGGTGCTGTGCCGGCCGCTCGGCCTGTGTCTCC TGCGTGTCTTAACGTCCCTTGTGTCCGGATTTCCATTTCAGATCAAGGGAATGAAGGACCGCCTGGAGTT CTGGTGCACGGATGTGAAGAGCATGGAGATGCTGGTGGAGCACCAGGCCCATGACATCCTCACCTAGGGC CCCCTGGTTCCCCGTCGTGTCTCCTTTGACTCACCTGAGAGAGGCGTTTGCAGCCAATGAAGCTGGCTGC TCAGACGGTCGACATTGAATTTGGGTGGGGGTTGGGATCCTGTCTGAAGTACAGACTGTTCTTGCTCTAA AAACAGGACTGTCCCTGATGGGAGCCAGGCCACAGGGAGGAGGCTTCTTTGTGGGTCTCTCCTGCAGAGG GTGGGGGTCTCAGGGTCTTAGGTGATACGGGAGAGAAAGAACGTGCCAGGCAGGAGGCCCCCTGAAGTCT GTGTACTCCGAGGTGGATCTCCATCCCCATCCACCTGTACGGACATCTTTTCCGTTGCGGTTTGAGAATG TTCCTATAATAAACCCCTCTGCTTTGTTCT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092845 unedited CCGAATTTGTAATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGCTCTTA GCTCAGACGACACCCTCCCACCCCACTGCCACCACCCTGAGGCCTTCCCTGACCATCTGA ACTGAGATGAAGTAGCTGCCCCTCTCCCCGTGATGCAGTTGTTGCCTCATTGCATGTCGC TTCTTGTGTACACGCACATCTGTCTTTCCCCACTAGAACTTAAGCTTCGTCAGGAAGTGG CCCTTAGATTTCTGGTTTGCCGTGGTCACCCCTGGTCAACCCTGGCAAATTGTGGCCTCA AGTACTTGTTCGGGGATTGAACAATGGCTACTGTCAGAAACTCCTGTAAGGCATTTTGCT GTTATCCTTCTTATCTAAGCATTAAAAGCTTGTTCTTAACAAGATATATGTCTAATATTA GGAAACTTTTTAGTATGTGGGGTACTAATAAGATCTCTATTTTCAGAGCCAATATTGACA AAACATCCTTATCAGTTCTCTATTTTTATTTGGAAAAATAGTTTAAAATAGTTAATAAAA TTTTTTCCAGATGACTTTCACACGACCTGCCAATCACAGACAACTCACTTTTGAAGAAAT TGCCAAAAGTGCTAAAATCACAGTGAATGAGGTACGGTCCCTAGGCTCAGGGTGTTAGAG CAGCAAAGCTGCCACATGGAGGAGTCAAGGCTCTTGTGTGAGCGCTGTGCTCCCTAGACA GTAAAAAATGACCGGAGGAGCNGCATCTGCTTCTCACANAGCCATCTGGTCCCATGGGGG TGTGTCAGGCTATCTTTGTCTACACACGCCTCCCTCTCTGCACAGGTGGAGCTTCTGGTG ATGAAAGCCCTTTTCGTGGNNGCTGTGAAAGGCAGTATANACGAAGTGGACAAACGAGTC ACATGACCCTGGTGCAGNCCCCGAGTGTGNNNATTGCACAGTGATGTGTTTGAAACCATN ATCCACTCTGTGATTTGAGGGGTGTGTCTATACGNCTAGTTCATTGGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092845 unedited NGGTTCGACTAAGNNANCGCGGNCCCGCATTCNANNGATCGATTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACAAACCCGA GGGGTTTTTTTTAGGAACATTTTCAAACCCCAACGGAAAAAAATTTCCCTCCCGGTGGAT GGGGATGGAAATCCCCCTCGGAGTACACAAACTTTAGGGGGCCCCCTGCCTGGCACGTTT TTTTTTTCCCGTTTCACCTAAAACCCTGAAACCCCCCCCCTTTGGGGGAAAAACCCCCAA AAAAACCCCCTCCCTGGGGCCTGGCTCCCATCAGGGACAGTCCTGTTTTTAAAACAAAAA CAGTTTGTTCTTTAAACAGGATCCCCACCCCCCCCCAAATTTAATGTCCACCGTTTGAAC ACCCCGCTTTTTTGGCTGCAAACCCCTTTTTCAGGGGAGTCAAAGGAAACCCCACGGGGA ACCCGGGGGCCCTAAGTGAGGATGTCATGGGCCTGGTGCTCCACCAGCATTTCCATGCTT TTTACATCCGGGCCCCAAAACTCCAGGCGGGCCTTCATTCCCTTGATCTGAAATGGAAAT CCCGACCCCAGGGACCTTAAAACCCCCAGGAAAAACAGGCCGAGCGGCCGGGACAACACC TGATGGGGGGACTTTACATTTCCAGCTAACTTTTTTGGGCAGCTTTTGTAAAAAGGACCT CTTAACTGGGGGAATGGGACCGGACTTTCCCGGTTAATTTTTTTCCAGGAACCTTTAAAA AAACTTGCCTGAAAGAAAGCCGACACGGGCCGAGGAAATCCCCCGGATCGCCTTTCCCGC AATGTTAGAACCACGTGGGCTTCAATCCAGAACCCGGACGTTTCTGATCAAACTCCTATC TTAAGATTAAAAGGCCCGTN
Restriction Sites	NotI-NotI
ACCN	AK092845
Insert Size	2200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK092845.1
RefSeq Size	2200 bp
RefSeq ORF	2200 bp
Locus ID	5719
Protein Pathways	Proteasome
Gene Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

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