PSMC5 (AK098484) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	p45; p45/SUG; S8; SUG-1; SUG1; TBP10; TRIP1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK098484, the custom clone sequence may differ by one or more nucleotides GGATGCCGGCGGTCTCTGCTGAAGAGAGAAGATGGCGCTTGACGGACCAGAGCAGGTATGGCGGGTGCAG TGGCGGCCCGGCAGGTTACGGGGCTGGGTGCGGAGCGAGCGTGATCTGAGTGGAGAGCGGGCCGGGGCAG GAGCGTCGGGTGGGTCCATGGATTTTCAATTTCACCCTAACCCCCTGCGTTGTTCAAGTGTCAACTGTAA TATAAATTGGATGGGGGAAAGCCACAAAAGACAATTCTGAATTCCAAGTGCATTTGCAGGTATTATGTTT AGTTTACCATTTTTGAATGCTTTTTCATGTGTTTTTATTCGTTCACCTTAGTAGTCTTGAAGCAGGGTAG CTGATGCTATACCCATGTGCCGTTTGGTAACAGATCTAGATTTTTAAAAATAGATTGTCTTACCTTTACT TTGCTTTTGATTGACAGCGGCTGGGCTAGGACTCAGGTTTCTCACCTAACAATCTTTCCACCAGGTGTTG TTGCCTTCCTGCAAGAAATTTGGTGTTACATGAGCCAGCTGTAACTTTTCTCCATTAGATTCTGTTACTC CTGGACCAGTATTGGACAAGCAGCCTTCTTTGATGGCTGCTGTAAGGTCCAGAGGGACTCTTGAAGGACA GGGCAGGGTCATTAGGATCATACTGGAGAATCTGAAACAACATGCAGGTGCCCTGTGCCCTTCCCTCAGC TCATTCAGACCTGTGAGGTCTTGGCCAAGATCCTACCTCCTCCTGTCTCCTGCCCTTGAATGATGGAATT TGTCTCTTGTCTGCCACAGCTGATTGTGAATGATAAGAGCCAAAACCTCCGGAGGCTGCAGGCACAGAGG AACGAACTAAATGCTAAAGGTGAGTGGAGAAAGATGGGAAGCCGCATGTGGGCAGTTTGTCTGAGGTCTG TCCTTATCTCCCTGCACTGTGTCTTCACAGCAGTGCTTACTGTTTTCTCCCTGTCATCATCTAGTAGTTT CACATGCATCTCTTTTTCCTGAGCCCTATTGTAGCCTCCTTAAATACATGAATTTTGACCTTTGGCCTTC CTTTAATAAGAGTGATGCTTAGCTCATGCACGTAGAATTGGGTCCTGGAGACTCCAAAGGAGTAGCTAGG TGACCACTGTGTCTGTTTGCCATCTAGTTCGCCTATTGCGGGAGGAGCTACAGCTGCTGCAGGAGCAGGG CTCCTATGTGGGGGAAGTAGTCCGGGCCATGGATAAGAAGAAAGTGTTGGTCAAGGTAAAAGCAGCATGA CCCCAGGGACCAGCTCGGTCTCCACTGCATTCCCACCCCTTTGTGTGTAGCCTCGGGAGACAGGGTTCTG TGTTCTGTCAAGGTATTGTGGGATTCTCATAGGTCTTCCTGGGTAGGATTAGTGATTTGGTGGCTGTCAA GGAAGGTCATGAGCCCTTGTTTCTTTAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGACAAAAAC ATTGACATCAATGATGTGAGTGTAGCAGGTGAGGTGGTGGTGGTGGTGGGGTCAGCTCTTACCGTACCAC TTCTGAAACTCGCCCCCTTCACCCAGGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGCTACAC TCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTGGAGAAAGTACCAGAT TCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAGATCAAAGAAGTGATCGAGCTGCCTG TTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATTGCTCAGCCCAAGGGAGTGCTGCTGTATGGACCTCC AGGCACTGGGAAGACACTGTTGGCCCGGGCTGTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCT GGCTCTGAACTGGTACAGAAATTCATAGGGGAAGGTAACCATGGCTAGCAATGTGAGAAGCAAGAGGTAG GGGTAGGGGGTTAGAGAGCTAATAAGCTAATAAGCTCCCTAACACCAGCTCGGCCTCCACACAGGGGCAA GAATGGTGAGGGAGCTGTTTGTCATGGCACGGGAACATGCTCCATCTATCATCTTCATGGACGAAATCGA CTCCATCTCTTTGAGGGTCTTAGTGTTCACAGTTTGTTACACATCTTCGAGTCAAATCTTAATAGTAAAG GTACCTCTAGCCAAAGGTCAACCCTTTTGGAAGAAGCCCGAATGAAAAGGATTCTTATAAAAGGGAAACA TAAGCTCAGGAGTTGAAATTTATCACCAAGAGGTTGGGTGAGGGTGAAGTGAGAATGTGAGGAAGGGCAT CATTGAGGGATCTGGACAATGCTAATTCCATAGAGACTGAATGAAAGAAATTGGGCAACCCAGAGCCCTA GCCTAAAGCGCTAAACCATAGAAAAAGGACCGTTTATGTTGTTAATCAAATGTTTGTGTTACCAGTAAGG CTTCCAGCAGTAAGGTATGGCTTCCAGTAGTAAGCCTTACAGACAACATAAACAGTAGTCTGTTAGTGTT ACGTTATACTGTTACGGTAGGCTGTTAGTAGTTTAACTTTGGACAGGTCCATGGATTTTCAATTTCACCC TAACCCCCTGCGTTGTTCAAGTGTCAACTGTAATATAAATTGGATGGGGGAAAGCCACAAAAGACAATTC TGAATTCCAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTCGACGGCTTTGAGGCCACCAAGAAC ATCAAGGTTATCATGGCTACTAATAGGATTGATATCCTGGACTCGGCACTGCTTCGCCCAGGGCGCATTG ACAGAAAAATTGAATTCCCACCCCCCAATGAGGAGGCCCGGCTGGACATTTTGAAGATTCATTCTCGGAA GATGAACCTGACCCGGGGGATCAACCTGAGAAAAATTGCTGAGCTCATGCCAGGAGCATCAGGGGCTGAA GTGAAGGGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAACGGCGAGTCCATGTCACTCAGGAGG ACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGGACAGTGAGAAAAACATGTCCATCAAGAAATTATG GAAGTGAGTGGACAGCCTTTGTGTGTATCTCTCCAATAAAGCTCTGTGGGCCAAGTCC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK098484 unedited ACACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCTGAAGAGAAAAAGGCG CTTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTCCGCCAA TATTATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAAAACCTC CGGAGGCTGCAGGCACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGGGAGGAG CTACAGCTGCTGCAGGAGCAGGGCTCCTATGTGGGGGAAGTAGTCCGGGCCATGGATAAG AAGAAAGTGTTGGTCAAGGTACATCCTGAAGGTAAATTTGTTGTAGACGTGGACAAAAAC ATTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAGGAATGACAGCTACACT CTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTGGAGAAA GTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAGATCAAA GAAGTGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATTGCTCAG CCCAAGGGAGTGCTGCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCCCGGGCT GTGGCTCATCATACGGACTGTACCTTTATTCGTGTCTCTGGCTCTGNATTGGTACAGAAA TTCATAGGGGAAGGGGGCAGAATGGTGANGGGAGCTNGTTGTCATGGCACGGGAACATGC TCCATCTATCATCTTCATGGACGAAATCGACTCCCATCGCTTCTCGCGCTGGAGAGGGGG GTTCTGGAGGNGACAANTGAANTGCACGCACGATGCCTGNAGTGCTCACCANCTCGACGC TTTTGAGGCACCAGACATCAGGNTATCATGGCTCTAATAGGATNGANTCN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK098484 unedited NGGTACTACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAC TTGGCCCACANAGCTTTATTGGAGAGNAACACACAAAGGCTGTCCACTCACTTCCATAAT TTCTTGATGGACATGTTTTTCTCACTGTCCTTCTGCATGACCTTGGCTACTGCCATCTCA AAGTCCTCCTGAGTGACATGGACTCGCCGTTCTCGCAGGGCATACATGCCAGCTTCTGTG CACACGCCCTTCACTTCAGCCCCTGATGCTCCTGGCATGAGCTCAGCAATTTTTCTCAGG TTGATCCCCCGGGTCAGGTTCATCTTCCGAGAATGAATCTTCAAAATGTCCAGCCGGGCC TCCTCATTGGGGGGTGGGAATTCAATTTTTCTGTCAATGCGCCCTGGGCGAAGCAGTGCC GAGTCCAGGATATCAATCCTATTAGTAGCCATGATAACCTTGATGTTCTTGGTGGCCTCA AAGCCGTCGAGCTGGTTGAGCAACTCCAGCATCGTGCGCTGCACTTCACTGTCCCCTCCA GAACCCCCCTCCAGCCGCGAGGAGCCGATGGAGTCGATTTCGTCCATGAAGATGATAGAT GGAGCATGTTCCCGTGCCATGACAAACAGCTCCCTCACCATTCTTGCCCCTTCCCCTATG AATTTCTGTACCAATTCAGAGCCAGAGACACGAATAAAGGTACAGTCCGTATGATGAGCC ACAGCCCGGGCCAACAGTGTCTTCCCAGTGCCTGGAGGTCCATACAGCAGCACTCCCTTG GGCTGAGCATGCCCAGTGCTTCGAAGAGCTCAGGATGCTTNACAGGCAGCTCGATCACTT CTTGATCTCCTTGATCTGGTTGTCCAGTCCACCATCATCTCATAAGTTGGATCTGGGACT TTTCTCCCCN
Restriction Sites	NotI-NotI
ACCN	AK098484
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK098484.1
RefSeq Size	3138 bp
RefSeq ORF	3138 bp
Locus ID	5705
Domains	AAA
Protein Families	Druggable Genome
Protein Pathways	Proteasome
Gene Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

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