United States
Germany
Japan
United Kingdom
China
Cytochrome P450 Reductase (POR) (NM_000941) Human Untagged Clone
CAT＃: SC100401
POR (untagged)-Human P450 (cytochrome) oxidoreductase (POR)
CNY 2,950.00
| Cited in 5 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CPR; CYPOR; P450R |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC100401 sequence for NM_000941 edited (data generated by NextGen Sequencing)
ATGATCAACATGGGAGACTCCCACGTGGACACCAGCTCCACCGTGTCCGAGGCGGTGGCC GAAGAAGTATCTCTTTTCAGCATGACGGACATGATTCTGTTTTCGCTCATCGTGGGTCTC CTAACCTACTGGTTCCTCTTCAGAAAGAAAAAAGAAGAAGTCCCCGAGTTCACCAAAATT CAGACATTGACCTCCTCTGTCAGAGAGAGCAGCTTTGTGGAAAAGATGAAGAAAACGGGG AGGAACATCATCGTGTTCTACGGCTCCCAGACGGGGACTGCAGAGGAGTTTGCCAACCGC CTGTCCAAGGACGCCCACCGCTACGGGATGCGAGGCATGTCAGCGGACCCTGAGGAGTAT GACCTGGCCGACCTGAGCAGCCTGCCGGAGATCGACAACGCCCTGGTGGTTTTCTGCATG GCCACCTACGGTGAGGGAGACCCCACCGACAATGCCCAGGACTTCTACGACTGGCTGCAG GAGACAGACGTGGATCTCTCTGGGGTCAAGTTCGCGGTGTTTGGTCTTGGGAACAAGACC TACGAGCACTTCAATGCCATGGGCAAGTACGTGGACAAGCGGCTGGAGCAGCTCGGCGCC CAGCGCATCTTTGAGCTGGGGTTGGGCGACGACGATGGGAACTTGGAGGAGGACTTCATC ACCTGGCGAGAGCAGTTCTGGCCGGCCGTGTGTGAACACTTTGGGGTGGAAGCCACTGGC GAGGAGTCCAGCATTCGCCAGTACGAGCTTGTGGTCCACACCGACATAGATGCGGCCAAG GTGTACATGGGGGAGATGGGCCGGCTGAAGAGCTACGAGAACCAGAAGCCCCCCTTTGAT GCCAAGAATCCGTTCCTGGCTGCAGTCACCACCAACCGGAAGCTGAACCAGGGAACCGAG CGCCACCTCATGCACCTGGAATTGGACATCTCGGACTCCAAAATCAGGTATGAATCTGGG GACCACGTGGCTGTGTACCCAGCCAACGACTCTGCTCTCGTCAACCAGCTGGGCAAAATC CTGGGTGCCGACTTGGACGTCGTCATGTCCCTGAACAACCTGGATGAGGAGTCCAACAAG AAGCACCCATTCCCGTGCCCTACGTCCTACCGCACGGCCCTCACCTACTACCTGGACATC ACCAACCCGCCGCGTACCAACGTGCTGTACGAGCTGGCGCAGTACGCCTCGGAGCCCTCG GAGCAGGAGCTGCTGCGCAAGATGGCCTCCTCCTCCGGCGAGGGCAAGGAGCTGTACCTG AGCTGGGTGGTGGAGGCCCGGAGGCACATCCTGGCCATCCTGCAGGACTGCCCGTCCCTG CGGCCCCCCATCGACCACCTGTGTGAGCTGCTGCCGCGCCTGCAGGCCCGCTACTACTCC ATCGCCTCATCCTCCAAGGTCCACCCCAACTCTGTGCACATCTGTGCGGTGGTTGTGGAG TACGAGACCAAGGCCGGCCGCATCAACAAGGGCGTGGCCACCAACTGGCTGCGGGCCAAG GAGCCTGCCGGGGAGAACGGCGGCCGTGCGCTGGTGCCCATGTTCGTGCGCAAGTCCCAG TTCCGCCTGCCCTTCAAGGCCACCACGCCTGTCATCATGGTGGGCCCCGGCACCGGGGTG GCACCCTTCATAGGCTTCATCCAGGAGCGGGCCTGGCTGCGACAGCAGGGCAAGGAGGTG GGGGAGACGCTGCTGTACTACGGCTGCCGCCGCTCGGATGAGGACTACCTGTACCGGGAG GAGCTGGCGCAGTTCCACAGGGACGGTGCGCTCACCCAGCTCAACGTGGCCTTCTCCCGG GAGCAGTCCCACAAGGTCTACGTCCAGCACCTGCTAAAGCAAGACCGAGAGCACCTGTGG AAGTTGATCGAAGGCGGTGCCCACATCTACGTCTGTGGGGATGCACGGAACATGGCCAGG GATGTGCAGAACACCTTCTACGACATCGTGGCTGAGCTCGGGGCCATGGAGCACGCGCAG GCGGTGGACTACATCAAGAAACTGATGACCAAGGGCCGCTACTCCCTGGACGTGTGGAGC TAG Clone variation with respect to NM_000941.2 387 a=>g;1033 c=>t;1455 t=>c >OriGene 5' read for NM_000941 unedited
NNGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGCCAG CGTTTCATGATCAACATGGNGAGACTCCCACGTGGACACCAGCTCCACCGTGTCCGAGGC GGTGGCCGAAGAAGTATCTCTTTTCAGCATGACGGACATGATTCTGTTTTCGCTCATCGT GGGTCTCCTAACCTACTGGTTCCTCTTCAGAAAGAAAAAAGAAGAAGTCCCCGAGTTCAC CAAAATTCAGACATTGACCTCCTCTGTCAGAGAGAGCAGCTTTGTGGAAAAGATGAAGAA AACGGGGAGGAACATCATCGTGTTCTACGGCTCCCAGACGGGGACTGCAGAGGAGTTTGC CAACCGCCTGTCCAAGGACGCCCACCGCTACGGGATGCGAGGCATGTCAGCGGACCCTGA GGAGTATGACCTGGCCGACCTGAGCAGCCTGCCGGAGATCGACAACGCCCTGGTGGTTTT CTGCATGGCCACCTACGGTGAGGGAGACCCCACCGACAATGCCCAGGACTTCTACGACTG GCTGCAGGAGACAGACGTGGATCTCTCTGGGGTCAAGTTCGCGGTGTTTGGTCTTGGGAA CAAGACCTACGAGCACTTCAATGCCATGGGCAAGTACGTGGACAAGCGGCTGGAGCAGCT CGGCGCCCAGCGCATCTTTGAGCTGGGGTTGGGCGACGACGATGGGAACTTGGAGGANGA CTTCATCACCTGGCGAGAGCAGTTCTGGCCGGCCGTGTGTGAACACTNNTGGGTGGAAGC CACTGGCNGAGAGTCCAGCATTCGCCAGTACGAGCTTNGGGNNTCACACCGACATAGATG CCGCCAAGGTGTACATGGNNGGAGAATGGNCCCNNGCTGAAAGCTACNANAACCANAAGC CCCCCCTTGATGCCAGNAATCCGNTCCTGGCTGCAGTCACACCCACCGGAGCTGAA >OriGene 3' read for NM_000941 unedited
TNAAGATCGAGATTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTACAGAAAAACAAA ACTTTATTCCAGGGCCAGAGGTTATTTAAAATTATTTACACTCACTGGGAAATCACGTGG AGGGGCTGGGCCCAGCTCAGCAGAAAAAAAAAAGCCCTTCTGTGCAGCCACCGAGGGCCA GAGGCCTCCAGAGGCATGGAGCCCGGTGCCCCCATGCAGGCCCTGGGCTGTGCTGCCAGG GGCCGGTGGACCTCACCTGGCCTGGGGGCTGAGGAGGATGCACCCCAGGCCCAAAGGAGT CTTTGTCACTGGGCCTGCGCCCATGCCAAGGCCAAGCCAAACACACCCAGGAGACTACGG GAGGGAGCCAGGAAAGCTGATTACAGGCCGGATTCTGTGGGGTGGGTGGGGCAAGCAGGC CCCTAGCTCCACACGTCCAGGGAGTAGCGGCCCTTGGTCATCAGTTTCTTGATGTAGTCC ACCGCCTGCGCGTGCTCCATGGCCCCGAGCTCAGCCACGATGTCGTATAAGGTGTTCTGC ACATCCCTGGCCATGTTCCGTGCATCCCCACAGACGTAGATGTGGGCACCGCCTTCGATC AACTTCCACAGGTGCTCTCGGTCTTGCTTTAACAGGTGCTGGACGTAGACCTTGTGGGAC TGCTCCCGGGAGAAGCCACGTTGAGCTGGGTGAGCGCACCGTCCCTGTGGAACTGCGCCA GCTCCTCCCGGTACAGGTAGTCCTCATCCGAGCGGCGGCAGCCGTAATACAAAAGCGTCT CCCCCACCTTCTTGCCCTGCTGGTCGCACCCAGCCCCGTCCTGGGAGAAACA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_000941 |
| Insert Size | 2650 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000941.1, NP_000932.1 |
| RefSeq Size | 2446 bp |
| RefSeq ORF | 2043 bp |
| Locus ID | 5447 |
| UniProt ID | P16435 |
| Domains | flavodoxin, NAD_binding_1, FAD_binding_1 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Gene Summary | This gene encodes an endoplasmic reticulum membrane oxidoreductase that is essential for multiple metabolic processes, including reactions catalyzed by cytochrome P450 proteins for metabolism of steroid hormones, drugs and xenobiotics. The encoded protein has a flavin adenine dinucleotide (FAD)-binding domain and a flavodoxin-like domain which bind two cofactors, FAD and FMN, that allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene cause a complex set of disorders, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome, that resemble those caused by defects in steroid metabolizing enzymes such as aromatase, 21-hydroxylase, and 17 alpha-hydroxylase. [provided by RefSeq, Aug 2020] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in higher primate species. This starting position is most commonly referred to in the literature, and the numbering system used to describe disease-associated mutations is based on this protein start. This includes data in the P450 oxidoreductase (POR) allele nomenclature locus-specific database, and the Human Gene Mutation Database (HGMD). This start codon is restricted to higher primate species, and it has a weak Kozak signal. However, it should be noted that an alternative downstream start codon, which has a strong Kozak signal and is much more widely conserved, is also present. The use of this downstream start codon would result in a protein that is 3 aa shorter at the N-terminus. Protein sequencing in PMID:2513880 indicates that this is the preferred start codon in vivo. It is therefore possible that the ribosome will initiate at the upstream start codon only a small fraction of the time (if at all), while leaky scanning will allow the downstream start codon to be used predominantly. |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Enzymatic Activities of CYP3A4 Allelic Variants on Quinine 3-Hydroxylation In Vitro
,Zhou, XY;Hu, XX;Wang, CC;Lu, XR;Chen, Z;Liu, Q;Hu, GX;Cai, JP;,
Front Pharmacol
,PubMed ID 31214030
[POR]
|
|
A CRISPR screen identifies a pathway required for paraquat-induced cell death
,Reczek, CR;Birsoy, K;Kong, H;Martínez-Reyes, I;Wang, T;Gao, P;Sabatini, DM;Chandel, NS;,
Nat. Chem. Biol.
,PubMed ID 29058724
[POR]
|
|
Identification and characterization of a novel CYP2C9 allelic variant in a warfarin-sensitive patient
,Dai, DP;Li, CB;Wang, SH;Cai, J;Geng, PW;Zhou, YF;Hu, GX;Cai, JP;,
Pharmacogenomics
,PubMed ID 26255664
[POR]
|
|
In vitro functional characterization of 37 CYP2C9 allelic isoforms found in Chinese Han population
,Dai, DP;Wang, YH;Wang, SH;Geng, PW;Hu, LM;Hu, GX;Cai, JP;,
,PubMed ID 24077631
[POR]
|
|
MicroRNA-205 suppresses the oral carcinoma oncogenic activity via down-regulation of Axin-2 in KB human oral cancer cell
,Kim, JS;Park, SY;Lee, SA;Park, MG;Yu, SK;Lee, MH;Park, MR;Kim, SG;Oh, JS;Lee, SY;Kim, CS;Kim, HJ;Chun, HS;Kim, JS;Moon, SM;Kim, DK;,
Mol Cell Biochem. 2013 Oct 29
,PubMed ID 24166197
[POR]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202172 | POR (Myc-DDK-tagged)-Human P450 (cytochrome) oxidoreductase (POR) |
CNY 3,990.00 |
|
| RC202172L1 | Lenti ORF clone of Human P450 (cytochrome) oxidoreductase (POR), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202172L2 | Lenti ORF clone of Human P450 (cytochrome) oxidoreductase (POR), mGFP tagged |
CNY 5,890.00 |
|
| RC202172L3 | Lenti ORF clone of Human P450 (cytochrome) oxidoreductase (POR), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202172L4 | Lenti ORF clone of Human P450 (cytochrome) oxidoreductase (POR), mGFP tagged |
CNY 5,890.00 |
|
| RG202172 | POR (tGFP-tagged) - Human P450 (cytochrome) oxidoreductase (POR) |
CNY 4,370.00 |
|
| SC324169 | POR (untagged)-Human P450 (cytochrome) oxidoreductase (POR) |
CNY 4,992.00 |
其它Cytochrome P450 Reductase产品
