Pcdh15 (NM_001271377) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217693 representing NM_001271377 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCACAGTTTGCTCTGTGGAAGTGTATGCCCCATGGGATCCTCCTTGCCTCACTCTTGATAGTCA GCTGGGGCCAATATGATGATGACTGGCAATATGAGGATTGTAAACTAGCTAGGGGAGGACCACCAGCTAC CATTGTGGCCATTGATGAAGAGAGTCGGAATGGTACAATTCTGGTGGACAACATGTTGATTAAGGGGACT GCCGGAGGACCAGACCCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTACTGGACC CCGTTAAGCAGATGCTTTTCCTCAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCGATGAACATACA CTCCATTGTAGTCCAAGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTGATCTATCATGAAGTACGCATC GTGGTGCGGGACCGGAATGACAACTCACCCACATTCAAGCATGAGAGCTACTATGCCACCGTGAATGAGC TCACTCCGGTCGGCACCACAATATTCACGGGGTTTGCGGGAGACAACGGAGCCACAGACATAGACGATGG CCCTAACGGACAGATAGAATACGTCATTCAGTACAACCCAGAAGATCCGACATCCAATGACACCTTCGAA ATTCCACTCATGTTGACTGGCAACGTGGTGCTGAGGAAAAGACTCAACTACGAGGATAAGACTCGCTACT ACGTCATCATCCAAGCTAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACTACTACCACCCTCAC AGTAGACGTTCTAGATGGAGATGATCTGGGACCAATGTTTCTGCCCTGTGTTCTTGTGCCAAATACCCGT GACTGCCGTCCACTCACCTATCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTT TGGTGACCCCACCTATCCAAGCCATTGACCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCAT CCTCTACTCCATTCTTGTTGGCACCCCTGAGGATTACCCCCGCTTCCTCCACATGCATCCCAGGACTGCA GAACTCACTCTCCTGGAGCCAGTAAACAGAGACTTCCATCAAAAGTTTGATTTGGTTATTAAGGCCGAAC AGGACAATGGCCACCCGCTGCCTGCCTTTGCTAGTCTACACATCGAGATACTGGACGAAAATAATCAGAG TCCATACTTTACAATGCCCAGCTATCAAGGGTACATTCTGGAATCTGCCCCAGTGGGAGCCACCATTTCT GAGAGCCTGAACTTAACCACGCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACGTGCCACCTG GTGGAGTTCCTACAAAGGACCCGGAGCTCCACCTTTTCCTGAATGATTACACCTCGGTCTTCACCGTGAC ACCCACCGGTATCACCCGCTACCTCACCCTGCTTCAACCTGTGGACAGGGAGGAACAGCAAACCTATACC TTTCTGATAACAGCGTTCGATGGCGTGCAGGAGAGCGAGCCAGTGGTGGTCAATATCCGAGTGATGGATG CCAATGACAACACGCCCACCTTCCCTGAGATCTCCTACAATGTCTATGTTTACACGGACATGAGTCCCGG GGACAGTGTCATCCAGCTGACTGCAGTGGACGCTGATGAAGGCTCTAACGGGGAGATCTCCTATGAAATA CTAGTGGGCAGCAAGGGAGACTTCGTGATCAACAGGACCACGGGGCTGGTGAGCATCGCGCCAGGAGTGG AGCTGGTCGTGGGACAGACGTATGCGCTCACGGTACAGGCTTCGGACAACGCCCCGCCCGCAGAGAGAAG GCACTCCATCTGCACGGTGTACATCGAGGTGCTGCCTCCCAACAACCAGAGCCCTCCGCGTTTCCCACAG CTGATGTACAGTCTGGAAGTCAGCGAAGCCATGAGGATCGGCGCGGTTTTATTAAATCTACAGGCAACTG ATCGAGAGGGAGACCCAATCACATATGCCATCGAGAATGGAGACCCTCAGCGAGTTTTTAATCTTTCAGA AACCACAGGGATTCTCAGCCTAGGGAAGGCTCTAGATCGAGAGAGCACGGACCGCTACATCCTCATCGTC ACAGCCTCAGATGGCAGGCCAGATGGAACCTCAACTGCCACTGTGAACATAGTGGTGACAGACGTCAATG ATAACGCTCCCGTGTTCGATCCCTATCTGCCCAGGAACCTCTCTGTGGTGGAGGAAGAGGCCAATGCCTT TGTGGGTCAAGTCCGGGCGACAGACCCAGATGCTGGGATAAATGGCCAAGTGCACTACAGCCTGGGGAAT TTCAACAACCTCTTCCGCATCACGTCCAACGGGAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCA GGGACCACTATGAACTGGTTGTTGTGGCAACAGATGGAGCTGTACACCCTCGACATTCAACTCTGACACT GTACATCAAGGTGTTGGACATTGACGATAACAGCCCTGTTTTTACCAATTCAACGTACACGGTTGTTGTT GAAGAGAACCTGCCAGCCGGGACCGCCTTCCTTCAAATAGAGGCCAAGGATGTTGATCTTGGAGCCAATG TGTCCTATCGGATCAGAAGCCCGGAAGTGAAGCACCTCTTTGCACTGCATCCATTTACTGGGGAATTGTC TCTTCTGAGGAGTTTGGATTATGAGGCCTTTCCCGACCAAGAGGCAAGCATCACGTTCTTGGTGGAGGCC TTTGATATTTATGGGACGATGCCACCCGGTATAGCAACGGTCACAGTAATTGTGAAGGACATGAATGACT ACCCTCCGGTGTTTAGCAAACGCATCTACAAGGGGATGGTGGCTCCAGATGCCGTCAAGGGGACACCAAT TACCACTGTTTATGCTGAAGACGCAGACCCACCAGGGTTGCCAGCAAGTCGGGTGAGGTACCGAGTGGAT GATGTGCAGTTTCCATACCCGGCCAGTATTTTTGACGTAGAGGAAGATTCTGGAAGAGTAATAACCCGTG TTAATCTTAATGAAGAGCCTACAACAATTTTTAAGCTGGTGGTTGTGGCTTTTGATGATGGTGAACCTGT GATGTCCAGCAGTGCCACTGTGAGAATTCTTGTCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAG GAATACAGACCTCCTCCTGTGAGCGAGCTTGCTGCCAGAGGGACTGTAGTAGGCGTCATTTCTGCTGCCG CCATCAATCAGAGCATCGTGTACTCCATTGTGGCAGGAAATGAGGACGATAAATTTGGAATCAACAATGT CACTGGCGTCATCTATGTGAATTCCCCACTGGATTACGAGACAAGGACTAGCTATGTGCTCCGGGTGCAA GCAGATTCTCTGGAAGTGGTCCTCGCCAATCTCCGAGTCCCTTCAAAAAGCAATACAGCTAAGGTGTACA TTGAGATTCAGGATGAAAACGATCACCCCCCAGTGTTCCAGAAGAAATTCTACATTGGAGGTGTGTCTGA AGACGCGAGGATGTTCACGTCTGTGCTCAGAGTGAAGGCCACTGACAGGGACACAGGAAATTATAGCGCC ATGGCCTACAGACTCATCATACCACCGATTAAAGAGGGCAAAGAGGGGTTTGTGGTGGAAACATACACGG GGCTCATCAAGACAGCCATGCTCTTCCACAATATGAGAAGATCCTACTTCAAGTTTCAAGTGATTGCAAC CGACGACTACGGGAAGGGGTTGAGCGGGAAAGCGGACGTACTGGTCTCCGTGGTCAATCAACTGGATATG CAGGTCATTGTCTCCAATGTGCCCCCTACATTAGTGGAAAAGAAGATAGAAGATCTTACAGAGATTTTGG ATCGCTATGTTCAGGAGCAAATTCCTGGTGCCAAAGTTGTTGTAGAGTCCATTGGTGCCCGGCGCCATGG AGACGCCTTCTCCCTAGAAGACTATAGCAAGTGTGACCTGACCGTCTATGCCATCGACCCTCAGACCAAC AGAGCCATCGACAGAAATGAGCTTTTTAAGTTCCTGGACGGCAAACTGCTTGATATCAATAAAGACTTCC AGCCGTATTACGGGGAAGGAGGACGCATCCTGGAGATTCGGACTCCCGAAGCAGTGACGAGCATCAAGAA GCGAGGAGAGAGCTTGGGGTACACAGAAGGGGCCTTGCTGGCCTTGGCCTTCATCATTATCCTCTGCTGC ATCCCAGCCATCTTGGTCGTCTTAGTGAGCTACCGACAATTTAAAGTACGCCAGGCTGAGTGCACGAAGA CTGCAAGGATTCAGTCTGCTATGCCTGCAGCCAAGCCTGCAGCTCCTGTACCTGCTGCGCCCCCACCACC GCCGCCGCCACCACCACCAGGAGCACATCTCTATGAAGAACTGGGAGAGAGCGCCATGCATAAGTATGAG ATGCCCCAGTATGGAAGTCGCCGCCGACTGCTGCCACCTGCTGGACAGGAGGAATACGGCGAAGTCATTG GTGAAGCTGAAGAGGAGTATGAAGAGGAAGAGGTAAGTCCAGAGAAAGTTAAAAAGCCCAAAGTTGAAAT TAGAGAGCCTAGTGAGGAAGAGGTGGTAGTCACCGTTGAGAAGCCACCAGCGGCTGAGCCCACCTACCCG ACGTGGAAGAGAGCCAGGATCTTCCCAATGATCTTTAAGAAAGTCAGGGGTCTCGCTGAGAAAAGAGGCA TTGACCTTGAGGGCGAGGAGTGGAGGAGGCGCCTTGAGGAAGAAGACAAGGACTATCTCCAACTGACTCT AGACCAGGAGGAAGCCACGGAGAGCACCGTGGAGTCAGAGGAGGAGTCCAGCGACTACACGGAATACACA GAAACCGAGTCTGAGTTCAGCGAGTCCGAGACCACTGAAGAATCCGAGTCGGAGACCCCATCTGAGGAGG CAGAGGAAAGCTCGACCCCGGAGTCAGAGGAGTCTGAGTCCACCGAGTCAGAGGGAGAGAAGGCAAGGAA AAACATCGTGCTAGCAAGAAGAAGACCCGTGGTAGAAGAAATCCAAGAAGTGAAGGGTAAGAAAGAGGAG CCGCCTGTAGAGCAGGAAGAAGAGCCCCCGGGGGAGGAGGAAGAACGGCCAGAGGAAGGAGAAGAAAGCG AAGCAGCTCCCATGGATGAATCCACAGACCTGGAGGCTCAGGATGTCCCAGAGGAGGGCAGCGCAGAATC AGCCTCGGTGGAGAGGGGCGTGGAAAGTGAGGAGGAATCGGAGTCAGAAGTGAGCAGTAGCAGCAGCAGC AGCAGCAGTGAGAGCCTCTCTGGAGGCCCCTGGGGGTTCCAGGTACCAGAGTATGACAGGAGGAAGGAGG AAGATGCCAAGAAATCTCCAGGCGCAAACTCCGAAGGATACAACACAGCCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217693 representing NM_001271377 Red=Cloning site Green=Tags(s) MSPQFALWKCMPHGILLASLLIVSWGQYDDDWQYEDCKLARGGPPATIVAIDEESRNGTILVDNMLIKGT AGGPDPTIELSLKDNVDYWVLLDPVKQMLFLNSTGRVLDRDPPMNIHSIVVQVQCVNKKVGTVIYHEVRI VVRDRNDNSPTFKHESYYATVNELTPVGTTIFTGFAGDNGATDIDDGPNGQIEYVIQYNPEDPTSNDTFE IPLMLTGNVVLRKRLNYEDKTRYYVIIQANDRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTR DCRPLTYQAAIPELRTPEELNPILVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFLHMHPRTA ELTLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFASLHIEILDENNQSPYFTMPSYQGYILESAPVGATIS ESLNLTTPLRIVALDKDIEDVPPGGVPTKDPELHLFLNDYTSVFTVTPTGITRYLTLLQPVDREEQQTYT FLITAFDGVQESEPVVVNIRVMDANDNTPTFPEISYNVYVYTDMSPGDSVIQLTAVDADEGSNGEISYEI LVGSKGDFVINRTTGLVSIAPGVELVVGQTYALTVQASDNAPPAERRHSICTVYIEVLPPNNQSPPRFPQ LMYSLEVSEAMRIGAVLLNLQATDREGDPITYAIENGDPQRVFNLSETTGILSLGKALDRESTDRYILIV TASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVGQVRATDPDAGINGQVHYSLGN FNNLFRITSNGSIYTAVKLNREARDHYELVVVATDGAVHPRHSTLTLYIKVLDIDDNSPVFTNSTYTVVV EENLPAGTAFLQIEAKDVDLGANVSYRIRSPEVKHLFALHPFTGELSLLRSLDYEAFPDQEASITFLVEA FDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITTVYAEDADPPGLPASRVRYRVD DVQFPYPASIFDVEEDSGRVITRVNLNEEPTTIFKLVVVAFDDGEPVMSSSATVRILVLHPGEIPRFTQE EYRPPPVSELAARGTVVGVISAAAINQSIVYSIVAGNEDDKFGINNVTGVIYVNSPLDYETRTSYVLRVQ ADSLEVVLANLRVPSKSNTAKVYIEIQDENDHPPVFQKKFYIGGVSEDARMFTSVLRVKATDRDTGNYSA MAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHNMRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDM QVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDAFSLEDYSKCDLTVYAIDPQTN RAIDRNELFKFLDGKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRGESLGYTEGALLALAFIIILCC IPAILVVLVSYRQFKVRQAECTKTARIQSAMPAAKPAAPVPAAPPPPPPPPPPGAHLYEELGESAMHKYE MPQYGSRRRLLPPAGQEEYGEVIGEAEEEYEEEEVSPEKVKKPKVEIREPSEEEVVVTVEKPPAAEPTYP TWKRARIFPMIFKKVRGLAEKRGIDLEGEEWRRRLEEEDKDYLQLTLDQEEATESTVESEEESSDYTEYT ETESEFSESETTEESESETPSEEAEESSTPESEESESTESEGEKARKNIVLARRRPVVEEIQEVKGKKEE PPVEQEEEPPGEEEERPEEGEESEAAPMDESTDLEAQDVPEEGSAESASVERGVESEEESESEVSSSSSS SSSESLSGGPWGFQVPEYDRRKEEDAKKSPGANSEGYNTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271377
ORF Size	5373 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271377.1, NP_001258306.1
RefSeq Size	5376 bp
RefSeq ORF	5376 bp
Locus ID	690865
MW	198.8 kDa

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