Ciita (NM_001270803) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C2ta; Mhc2ta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217454 representing NM_001270803 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTGCCAGGTTCCCGGCCCTTCTGGGTCTTACCTGCAAGGCCATACTCTGTGTGCCACCATGGAGC TGGGGTCTCCAGAGGGCAGCTACCTGGAGCTCCTTAACAGTGATGCTGACCCCCTACACCTCTACCACCT CTATGACCAGATGGACCTTGCTGGGGAGGAAGAGATCGAACTCAACTCAGAGACAGACATGGACACCATC AACTGTGACCAGTTCAGCAAGCTGTTGCAGGATATGGAAATGGATGAAGAGACCCGGGAGGCCTATGCCA ATATCGCGGAACTGGATCAGTACGTGTTCCAGGATACCCAGCTGGAGGGCCTGAGCAAGGACCTCTTCAT ACAGCACATTGGACCAGAGGAAGGCTTTGGTGAGAACGTAGAGGGCCCTGCAGAATCAGGAGAGAAGCCT CAGAAGAGACGCTTCCCAGAAGAGCATGCTATGGACTCAAAGCACAGGAAACTAGTGCCTCCCTCTAGGA CCTCACTGAACTATTTGGATCTCCCCGCTGGGCATATCCAGATCTTCACCACTCTGCCCCAGGGACTCTG GCAAATCTCAGGGGTTGGCACAGGGCTCTCCAGTGTCCTAGTCTACCACGGTGAGATGCCCCAGGTCAAC CAATTACTCTCTTCAAGCAGCCCCACTGTCCCCAGTCTCCCCGAGTCCCCAGACCGGCCTGGCTCCACCA GCCCCTTCGCGCCATCCACAGCTGACCTGCCCGGCATGCCTGAACCTGCTCTGACCTCCCGTGTAAATGA GACAGAGGACACAACACCCCCCTCCCCATGCCAAGAGGATCCCGAGCCTTCCATCAAGCTTCCAAAATGG CCAGAGTCCGTGGAGCGGTTCCAGCACTCCCTACAGGACAAGTACCAAGCACTGCCCGGGAGCCCAAGTG GTCCTCTGGTAGCCATGGAGCTGGTGCGGGCCAGGTTGGAAAGAGGTAGCAACAAGAGTCAGGAAAGGGA GCTGGCCACTCCTGACTGGACAGAGCGCCAGCTAGCCCATGGCGGTCTGGCCGAGGTACTTCGGGTTGTC GGCGACTCCAGGAGATCACGAGAGACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGTCAGGGCAAGAGCC ACTGGGCCAGGACAGTGAGCCACATTTGGGCACAAGGCCAGTTACCACAATATGACTTTGTCTTCTACGT CCCCTGTCAATGCTTGGATCGCCCTGGGGACACCTACCACCTGCGGGATCTGCTCTGTCCCCCGAACCTG CAGCCACTGGCCATGGATGACGAGGTCATTGATCATATTGTGAGGCAGCCGGACCGTGTTCTGCTCATCC TAGATGCTTTTGAGGAGCTAGAGGCCCAAGACAGCCTCCTGCATGGACCCTGTGGACCTCTGTCCCCAGA GCCCTGCTCCCTCCGGGGACTGCTGGCTGGGCTCTTCCAGCGTAAGCTGCTGCGAGGCTGCACCCTGCTC CTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGATGCCATCTTCGAGGTGCCCA GCTTCTCTACGAAACAGGCCCAGATCTATATGAGGCACTACTTTGAGAACTCAGGGACAGCAGGGAGTCA CGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTTTTCTCCTCACCCACAGTCACAGCCCTGTTCTGTGC AGTGCCGTGTGTCAGCTCTCCAAGGCCCTGCTGGATCAGGGCGCAGAGGCCCAGCTACCTTGCACACTCA CAGGACTCTATGTTGGCCTGCTAGGCCCTGCAGCTCAGAACAGTCCTCCTGGAGCCTTAGTTGAGTTGGC CAAGCTAGCCTGGGAGCTGGGACGGAGGCACCAAAGCACCTTGCAGGAAACCCAGTTTCCATCCGTGGAA GTGAAGACCTGGGCTGTGGCCCAAGGCTTGATGCAGCAGACCACAGAGGCCCAACTGGCTTTCTCCAGTT TTCTGCTACAGTGCTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAGATGAAAGACAAGGAGCTGCC ACAGTACCTGGCCTTGACCCCGAGGAAGAAGCGACCCTATGACAACTGGCTGGAGGGCGTACCACGCTTT CTGGCTGGCTTAGTTTTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGCCTGCAATGGCTGCAG TGGCAGATAGGAAACGGAAGGTTCTTACCAGGTACCTGAAGCGCTTGAAGCTGGGGACACTTCGGGCCGG GAGGTTGCTGGAGCTGCTACACTGTGCCCACGAAACACAGGAACCTGGTATTTGGAAGCATGTTTCACAC CAGCTCCCAGGGCACCTCTCCTTCCTGGGCACACGGCTCACAGCCCCAGATGTGTATGTTCTGGGCAGGG CCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCTTCGTCAGACTGGCATCGAGCCCTCCAGACTGGG AGACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGACACAATGGCATTATGGGAG TCCCTACAGCATCAGGGAGAAACCCAGCTACTCCAGGCGGCAGAGGAGAAGTTCACCATTGAGCCATTTA AGGCCAAATCCCCAAAGGATGTGGAAGACCTGGACAGCCTTGTGCAGACCCAGAGGCTGAGAAACCCCTC AGCGGATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGAGTTTGCATTGGGCCCCGTC TTGGGCCCGCAGGCTTTCCCCACACTGGCCAAGATCCTTCCAGCATTCTCTTCCCTGCAGCACCTGGACC TGGACTCACTTAGCGAGAACAAGATCGGGGACAAGGGTGTGTCGAAGCTCTCAGCCACCTTCCCTCAGCT GAAGGCCCTGGAGACGCTCAACCTGTCCCAGAATAGCATCACCGATGTGGGAGCTTGCAAGCTTGCAGAA GCCCTGCCGGCCCTGGCCAAGTCCCTCCTAAGGCTGAGCTTGTACAACAACTGCATCTGTGACGAAGGGG CCAAGAGCCTGGCACGAGTGCTTCCAGACATGGTGTCCCTCCGGGTGATGGATGTCCAGTTCAACAAGTT CACGGCTGTCGGGGCCCAGCAGCTGACCTCCAGCCTTCAGAAGTGCCCTCAGGTGGAGACACTGGCAATG TGGACACCCACTATCCCCTTTGGGGTCCAGGAACACCTGCAGCAGCTGGATGCCCGGATTAGCCTGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217454 representing NM_001270803 Red=Cloning site Green=Tags(s) MRCQVPGPSGSYLQGHTLCATMELGSPEGSYLELLNSDADPLHLYHLYDQMDLAGEEEIELNSETDMDTI NCDQFSKLLQDMEMDEETREAYANIAELDQYVFQDTQLEGLSKDLFIQHIGPEEGFGENVEGPAESGEKP QKRRFPEEHAMDSKHRKLVPPSRTSLNYLDLPAGHIQIFTTLPQGLWQISGVGTGLSSVLVYHGEMPQVN QLLSSSSPTVPSLPESPDRPGSTSPFAPSTADLPGMPEPALTSRVNETEDTTPPSPCQEDPEPSIKLPKW PESVERFQHSLQDKYQALPGSPSGPLVAMELVRARLERGSNKSQERELATPDWTERQLAHGGLAEVLRVV GDSRRSRETQVVAVLGKAGQGKSHWARTVSHIWAQGQLPQYDFVFYVPCQCLDRPGDTYHLRDLLCPPNL QPLAMDDEVIDHIVRQPDRVLLILDAFEELEAQDSLLHGPCGPLSPEPCSLRGLLAGLFQRKLLRGCTLL LTARPRGRLAQSLSKADAIFEVPSFSTKQAQIYMRHYFENSGTAGSHDKALGLLEGQPFLLTHSHSPVLC SAVCQLSKALLDQGAEAQLPCTLTGLYVGLLGPAAQNSPPGALVELAKLAWELGRRHQSTLQETQFPSVE VKTWAVAQGLMQQTTEAQLAFSSFLLQCFLGAVWLAQCNEMKDKELPQYLALTPRKKRPYDNWLEGVPRF LAGLVFQPRAHCLGALVEPAMAAVADRKRKVLTRYLKRLKLGTLRAGRLLELLHCAHETQEPGIWKHVSH QLPGHLSFLGTRLTAPDVYVLGRALETASQDFSLDLRQTGIEPSRLGDLVGLSCVTSFRASLSDTMALWE SLQHQGETQLLQAAEEKFTIEPFKAKSPKDVEDLDSLVQTQRLRNPSADAAKDLPAIRDLKKLEFALGPV LGPQAFPTLAKILPAFSSLQHLDLDSLSENKIGDKGVSKLSATFPQLKALETLNLSQNSITDVGACKLAE ALPALAKSLLRLSLYNNCICDEGAKSLARVLPDMVSLRVMDVQFNKFTAVGAQQLTSSLQKCPQVETLAM WTPTIPFGVQEHLQQLDARISLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270803
ORF Size	3219 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270803.1, NP_001257732.1
RefSeq Size	5076 bp
RefSeq ORF	3222 bp
Locus ID	85483
MW	118.3 kDa
Gene Summary	may play a role in transcriptional activation of MHC class II genes [RGD, Feb 2006]

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