Slc8a1 (NM_001270776) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ncx; Ncx1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217377 representing NM_001270776 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCGACTAAGTCTCCCACCCAATGTTTCAATGGGATTTCGTCTGGTAACTCTGGTGGCTCTCTTGT TTACCCATGTTGACCATATAACTGCAGATACAGAGGCAGAAACAGGAGGAAATGAAACCACTGAATGTAC TGGCTCATATTACTGTAAGAAAGGGGTGATTCTGCCCATTTGGGAACCCCAAGACCCATCTTTTGGGGAT AAAATTGCTAGAGCAACTGTGTATTTTGTGGCCATGGTCTACATGTTCCTCGGAGTTTCTATTATTGCCG ACCGGTTTATGTCCTCTATAGAAGTCATCACCTCTCAAGAAAAGGAGATTACCATAAAGAAACCAAATGG AGAGACCACCAAGACTACAGTGCGTATCTGGAATGAGACTGTGTCCAACCTGACCTTGATGGCCCTGGGA TCTTCCGCTCCTGAGATTCTCCTGTCTGTCATTGAAGTGTGTGGCCATAACTTCACCGCAGGGGACCTTG GTCCCAGCACCATTGTGGGAAGCGCCGCCTTCAACATGTTCATCATCATCGCGCTTTGTGTTTATGTGGT CCCAGATGGAGAGACGAGGAAGATTAAACATCTGCGTGTGTTCTTTGTGACAGCAGCCTGGAGCATCTTT GCCTATACCTGGCTTTACATAATTTTGTCTGTCAGCTCTCCTGGTGTCGTGGAGGTCTGGGAAGGATTAC TCACTTTCTTCTTCTTTCCCATCTGTGTTGTGTTCGCTTGGGTTGCAGACAGGCGGCTTCTCTTTTACAA GTATGTCTACAAGCGGTACAGGGCTGGCAAGCAGAGGGGGATGATCATTGAACATGAAGGAGACAGACCA GCTTCCAAAACTGAAATTGAAATGGATGGGAAAGTAGTCAACTCCCACGTTGACAATTTCTTAGATGGCG CTCTGGTTTTGGAAGTCGATGAGAGGGACCAAGATGACGAGGAAGCCAGGCGTGAGATGGCAAGGATTCT GAAGGAACTTAAGCAGAAGCATCCCGACAAAGAGATCGAACAATTAATAGAATTAGCCAACTATCAAGTC CTAAGTCAGCAGCAAAAGAGCCGAGCATTTTACCGAATTCAAGCTACTCGCCTGATGACTGGAGCTGGTA ACATTTTGAAGAGGCATGCAGCTGACCAAGCGAGGAAGGCTGTCAGCATGCATGAAGTCAACATGGATGT GGTTGAAAATGACCCAGTCAGTAAGGTCTTCTTTGAGCAAGGGACATACCAGTGTCTAGAAAACTGTGGT ACTGTGGCCCTCACCATTATTCGAAGAGGGGGTGACTTGACCAACACTGTGTTTGTTGACTTCAGGACGG AAGATGGCACAGCCAATGCTGGGTCTGATTATGAGTTCACGGAAGGGACTGTGATCTTCAAACCTGGGGA GACCCAGAAGGAAATCAGAGTTGGCATCATTGATGATGATATCTTTGAAGAAGATGAAAACTTTCTTGTG CATCTTAGCAACGTCAGGGTCTCTTCAGAAGTCTCGGAAGATGGCATACTAGACTCCAATCACGTGTCTG CGATTGCTTGTCTCGGGTCACCCAACACTGCCACCATAACCATTTTTGATGATGACCACGCGGGCATCTT TACTTTCGAGGAACCCGTGACTCACGTGAGCGAGAGCATTGGCATCATGGAGGTGAAGGTGCTGAGAACC TCTGGAGCGCGAGGAAATGTTATCATTCCCTATAAAACCATTGAAGGCACAGCCCGAGGTGGAGGGGAGG ACTTTGAGGACACCTGTGGAGAGCTGGAATTCCAGAATGATGAAATAGTGAAGATCATTACCATTAGAAT ATTTGACCGTGAGGAATATGAGAAAGAGTGCAGTTTCTCCCTTGTGCTTGAGGAACCAAAATGGATAAGA AGAGGAATGAAAGCCCTGTTGTTAAATGAGCTTGGTGGCTTCACATTAACAGGAAAAAAGATGTATGGCC AACCTGTCTTCAGGAAGGTCCATGCTAGAGATCATCCGATTCCCTCTACCGTAATCAGCATTTCAGAGGA GTACGATGACAAGCAGCCACTGACCAGCAAAGAGGAGGAGGAGAGGCGCATTGCAGAAATGGGGCGCCCC ATTCTAGGCGAACACACCAAGCTGGAAGTGATCATTGAAGAGTCTTACGAATTCAAGAGCACTGTGGACA AACTCATTAAGAAGACGAACCTGGCCCTCGTGGTGGGGACCAACAGCTGGAGAGAGCAGTTCATTGAAGC GATCACCGTCAGCGCTGGGGAAGATGACGATGATGATGAATGTGGGGAGGAGAAGCTGCCCTCCTGTTTT GATTACGTGATGCACTTTCTCACAGTGTTCTGGAAGGTTCTGTTTGCCTTCGTCCCACCTACAGAATATT GGAATGGCTGGGCCTGCTTCATTGTCTCCATCCTCATGATCGGCCTACTGACAGCCTTCATTGGAGATCT GGCTTCCCACTTTGGCTGCACCATTGGTCTGAAAGATTCCGTGACTGCAGTTGTGTTTGTCGCTCTTGGA ACCTCAGTGCCAGACACATTTGCCAGCAAAGTAGCAGCTACCCAGGACCAGTATGCAGATGCGTCCATAG GCAATGTCACCGGAAGCAACGCTGTGAATGTCTTCCTGGGAATCGGCGTGGCCTGGTCCATTGCTGCCAT CTACCATGCGGCCAACGGGGAACAGTTCAAAGTGTCCCCTGGCACGCTAGCTTTCTCTGTCACTCTCTTC ACCATTTTTGCTTTCATCAACGTGGGGGTGCTGCTGTATCGGCGGAGGCCAGAAATAGGAGGTGAGCTGG GTGGGCCCCGGACTGCCAAGCTCCTCACATCTTCCCTGTTTGTGCTCCTGTGGCTCTTGTACATTTTCTT CTCCTCCCTGGAGGCCTACTGCCACATAAAAGGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217377 representing NM_001270776 Red=Cloning site Green=Tags(s) MLRLSLPPNVSMGFRLVTLVALLFTHVDHITADTEAETGGNETTECTGSYYCKKGVILPIWEPQDPSFGD KIARATVYFVAMVYMFLGVSIIADRFMSSIEVITSQEKEITIKKPNGETTKTTVRIWNETVSNLTLMALG SSAPEILLSVIEVCGHNFTAGDLGPSTIVGSAAFNMFIIIALCVYVVPDGETRKIKHLRVFFVTAAWSIF AYTWLYIILSVSSPGVVEVWEGLLTFFFFPICVVFAWVADRRLLFYKYVYKRYRAGKQRGMIIEHEGDRP ASKTEIEMDGKVVNSHVDNFLDGALVLEVDERDQDDEEARREMARILKELKQKHPDKEIEQLIELANYQV LSQQQKSRAFYRIQATRLMTGAGNILKRHAADQARKAVSMHEVNMDVVENDPVSKVFFEQGTYQCLENCG TVALTIIRRGGDLTNTVFVDFRTEDGTANAGSDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLV HLSNVRVSSEVSEDGILDSNHVSAIACLGSPNTATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRT SGARGNVIIPYKTIEGTARGGGEDFEDTCGELEFQNDEIVKIITIRIFDREEYEKECSFSLVLEEPKWIR RGMKALLLNELGGFTLTGKKMYGQPVFRKVHARDHPIPSTVISISEEYDDKQPLTSKEEEERRIAEMGRP ILGEHTKLEVIIEESYEFKSTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEDDDDDECGEEKLPSCF DYVMHFLTVFWKVLFAFVPPTEYWNGWACFIVSILMIGLLTAFIGDLASHFGCTIGLKDSVTAVVFVALG TSVPDTFASKVAATQDQYADASIGNVTGSNAVNVFLGIGVAWSIAAIYHAANGEQFKVSPGTLAFSVTLF TIFAFINVGVLLYRRRPEIGGELGGPRTAKLLTSSLFVLLWLLYIFFSSLEAYCHIKGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270776
ORF Size	2907 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270776.1, NP_001257705.1
RefSeq Size	6236 bp
RefSeq ORF	2910 bp
Locus ID	29715
UniProt ID	Q01728
MW	108.1 kDa
Gene Summary	displays Na+ gradient-dependent Ca2+ transport activity; plays a role in regulation of calcium ion transport [RGD, Feb 2006]

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