Trpc3 (NM_021771) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TrpC3c; Trrp3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217251 representing NM_021771 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGGCCGAGGGAGCCGAGCCGCAGCGCCGCCGCCGGGGCTGGAGGGGCGTCAACGGCGGGCTGGA GCCGCCCTGCCCGCGCGCGCCGCCGTCCCCGGGGCCCGACGCAGTACGGCAACATCCCTGTGGTGCGCAA GATGCTGGAGGAGTCTCGCACGCTCAATGTCAACTGCGTGGACTACATGGGCCAGAATGCGCTGCAGCTG GCCGTGGGCAATGAGCATCTGGAGGTGACCGAGCTGCTGCTGAAGAAGGAGAACCTGGCGCGTATCGGTG ATGCGCTGCTGCTCGCCATCAGCAAAGGCTATGTGCGCATCGTGGAGGCCATCCTAGGCCACCCAGGCTT TGCGGCCAGCCGGCGCCTGACCCTCAGCCCTTGCGAGCAAGAACTGCGCGATGACGACTTCTATGCTTAC GACGAGGATGGCACACGCTTCTCACCTGACATCACGCCCATCATCCTGGCTGCGCATTGCCATAAATACG AGGTGGTGCACCTGCTGCTACTCAAGGGTGCACGCATCGAGCGGCCACACGACTACTTCTGTCGCTGCGC CGACTGTGCTGAGAAGCAAAGGCTTGATGCCTTCAGCCACTCAAGGTCTAGGATCAATGCCTACAAGGGA CTGGCCAGCCCAGCATACTTGTCGCTGTCCAGCGAGGACCCTGTGCTCACAGCGCTGGAACTCAGCAATG AGCTGGCCAAGCTGGCCAACATAGAGAAGGAGTTCAAGAATGACTACAGGAAGCTCTCCATGCAGTGCAA AGACTTCGTAGTAGGTGTGCTGGACCTGTGCCGGGACTCAGAGGAGGTGGAAGCCATTCTGAATGGAGAT CTGGAATCGGTGGAACCCCTGGAGAGACACGGGCACAAGGCGTCGCTGAGTCGGGTCAAACTTGCCATTA AATATGAAGTCAAAAAGTTTGTGGCTCACCCCAACTGCCAACAGCAGCTTTTGACCATCTGGTACGAGAA CCTCTCGGGCCTTCGGGAGCAGACCATCGCTATCAAGTGTCTGGTCGTGTTGGTCGTGGCCTTGGGCCTT CCATTCCTCGCCATCGGCTACTGGATTGCACCTTGTAGCAGGCTGGGGAAAATTCTTCGAAGCCCCTTCA TGAAGTTCGTGGCTCACGCTGCCTCCTTCATCATCTTCCTGGGTCTGCTTGTGTTCAACGCCTCAGACCG GTTTGAAGGCATCACCACGCTGCCCAACATCACCGTTATTGACTACCCCAAGCAAATCTTCAGGGTGAAG ACCACCCAGTTCACATGGACAGAAATGCTAATTATGGTCTGGGTTCTCGGGATGATGTGGTCTGAGTGCA AGGAGCTGTGGCTGGAGGGGCCCCGGGAGTACATCGTGCAGCTGTGGAACGTGCTTGACTTCGGGATGCT CTCCATCTTCATTGCTGCCTTCACCGCCAGGTTCCTAGCGTTTCTGCAAGCCACCAAAGCGCAGCAGTAT GTGGACAGCCACGTGCAGGAGAGCGACCTGAGCGAAGTCACACTCCCACCAGAGGTTCAGTATTTCACCT ATGCTAGAGATAAATGGCTTCCTTCTGACCCTCAGATCATATCGGAAGGCCTCTATGCCATAGCTGTGGT GCTCAGCTTCTCCCGGATCGCGTACATTCTCCCTGCAAACGAGAGCTTTGGGCCCTTGCAGATCTCTCTG GGGAGGACTGTGAAGGACATATTCAAGTTCATGGTTCTCTTCATCATGGTGTTCCTGGCTTTCATGATTG GCATGTTCATACTTTACTCCTACTACCTTGGGGCCAAAGTAAACCCTGCTTTTACCACGGTTGAAGAAAG TTTCAAGACTTTGTTTTGGTCCATATTTGGACTCTCTGAAGTGACTTCTGTTGTCCTCAAATATGACCAC AAATTCATAGAGAACATTGGCTATGTCCTTTATGGAATATACAATGTAACTATGGTGGTCGTTCTGCTCA ACATGCTGATTGCTATGATTAACAGCTCATACCAAGAAATCGAGGATGACAGTGATGTAGAGTGGAAGTT TGCTCGTTCCAAACTCTGGCTATCCTACTTCGATGATGGAAAAACATTACCTCCACCCTTCAGTCTGGTC CCTAGTCCAAAATCGTTTGTTTATTTCATCATGAGGATCACTAACTTTTCCAAATGCAGGAGGAGAAGAC TTCAGAAGGATCTGGAACTGGGCATGGGTAACTCAAAGTCCAGGTTAAACCTCTTCACTCAGTCTAACTC GAGAGTTTTTGAATCACACAGTTTTAACAGCATTCTCAATCAGCCAACACGATATCAGCAGATAATGAAA AGACTCATAAAACGGTATGTTTTGAAAGCACAAGTAGACAAAGAAAATGATGAGGTGAACGAAGGTGAAC TGAAAGAAATCAAGCAGGATATCTCCAGCCTTCGTTACGAACTTTTGGAAGATAAGAGCCAAGCGACGGA GGAACTGGCCATCTTGATTCATAAACTCAGTGAGAAACTGAACCCCAGTGCGCTGAGGTGTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217251 representing NM_021771 Red=Cloning site Green=Tags(s) MARPREPSRSAAAGAGGASTAGWSRPARARRRPRGPTQYGNIPVVRKMLEESRTLNVNCVDYMGQNALQL AVGNEHLEVTELLLKKENLARIGDALLLAISKGYVRIVEAILGHPGFAASRRLTLSPCEQELRDDDFYAY DEDGTRFSPDITPIILAAHCHKYEVVHLLLLKGARIERPHDYFCRCADCAEKQRLDAFSHSRSRINAYKG LASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDSEEVEAILNGD LESVEPLERHGHKASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKCLVVLVVALGL PFLAIGYWIAPCSRLGKILRSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNITVIDYPKQIFRVK TTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYIVQLWNVLDFGMLSIFIAAFTARFLAFLQATKAQQY VDSHVQESDLSEVTLPPEVQYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISL GRTVKDIFKFMVLFIMVFLAFMIGMFILYSYYLGAKVNPAFTTVEESFKTLFWSIFGLSEVTSVVLKYDH KFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDGKTLPPPFSLV PSPKSFVYFIMRITNFSKCRRRRLQKDLELGMGNSKSRLNLFTQSNSRVFESHSFNSILNQPTRYQQIMK RLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKLNPSALRCE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021771
ORF Size	2514 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021771.2, NP_068539.2
RefSeq Size	3420 bp
RefSeq ORF	2517 bp
Locus ID	60395
MW	95.6 kDa
Gene Summary	a calcium-activated cation channel [RGD, Feb 2006]

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