Trpv2 (NM_001270798) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Vrl1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217180 representing NM_001270798 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCAGCCTCCAGCCCCCCAGCTTTCAGGCTGGAGACTTCCGATGGAGATGAAGAGGGCAATGCTG AGGTGAACAAGGGGAAGCAGGAACCGCCCCCCATGGAGTCACCATTCCAGAGGGAGGACCGGAATTCCTC CCCTCAGATCAAAGTGAACCTCAACTTCATAAAGAGACCTCCTAAAAACACTTCTGCTCCCAGCCAGCAG GAGCCAGATCGGTTTGACCGTGACCGACTCTTCAGTGTGGTCTCCCGGGGTGTCCCCGAGGAACTGACTG GACTGCTAGAATACCTGCGCTGGAACAGCAAGTACCTCACTGACTCTGCATACACAGAAGGCTCCACTGG AAAGACGTGCCTGATGAAGGCTGTGCTGAACCTTCAGGATGGGGTCAATGCCTGCATCATGCCGCTGCTG CAGATTGACAAGGATTCCGGCAATCCCAAGCCCCTCGTCAATGCCCAGTGCATCGATGAGTTCTACCAAG GCCACAGTGCGCTGCACATCGCCATAGAGAAGAGGAGCCTGCAGTGCGTGAAGCTGCTGGTAGAGAATGG AGCGGATGTTCACCTCCGAGCCTGTGGCCGCTTCTTCCAAAAGCACCAAGGAACTTGTTTCTATTTTGGA GAGCTACCTCTTTCTCTGGCTGCGTGCACCAAGCAGTGGGATGTGGTGACCTACCTCCTGGAGAACCCAC ACCAGCCGGCCAGCCTGGAGGCCACCGACTCCCTGGGCAACACAGTCCTGCATGCTCTGGTAATGATTGC AGATAACTCGCCTGAGAACAGTGCCCTGGTGATCCACATGTACGACGGGCTTCTACAAATGGGGGCGCGC CTCTGCCCCACTGTGCAGCTTGAGGAAATCTCCAACCACCAAGGCCTCACACCCCTGAAACTAGCCGCCA AGGAAGGCAAAATCGAGATTTTCAGGCACATTCTGCAGCGGGAATTCTCAGGACCGTACCAGCCCCTTTC CCGAAAGTTTACTGAGTGGTGTTACGGTCCTGTGCGGGTATCGCTGTACGACCTGTCCTCTGTGGACAGC TGGGAAAAGAACTCGGTGCTGGAGATCATCGCTTTTCATTGCAAGAGCCCGAACCGGCACCGCATGGTGG TTTTAGAACCACTGAACAAGCTTCTGCAGGAGAAATGGGATCGGCTCGTCTCAAGATTCTTCTTCAACTT CGCCTGCTACTTGGTCTACATGTTCATCTTCACCGTCGTTGCCTACCACCAGCCTTCCCTGGATCAGCCA GCCATCCCCTCATCAAAAGCGACTTTTGGGGAATCCATGCTGCTGCTGGGCCACATTCTGATCCTGCTTG GGGGTATTTACCTCTTACTGGGCCAGCTGTGGTACTTTTGGCGGCGGCGCCTGTTCATCTGGATCTCATT CATGGACAGCTACTTTGAAATCCTCTTTCTCCTTCAGGCTCTGCTCACAGTGCTGTCCCAGGTGCTGCGC TTCATGGAGACTGAATGGTACCTACCCCTGCTAGTGTTATCCCTAGTGCTGGGCTGGCTGAACCTGCTTT ACTACACACGGGGCTTTCAGCACACAGGCATCTACAGTGTCATGATCCAGAAGGTCATCCTTCGAGACCT GCTCCGTTTCCTGCTGGTCTACCTGGTCTTCCTTTTCGGCTTTGCTGTAGCCCTAGTAAGCTTGAGCAGA GAGGCCCGAAGTCCCAAAGCCCCTGAAGATAACAACTCCACAGTGACGGAACAGCCCACGGTGGGCCAGG AGGAGGAGCCAGCTCCATATCGGAGCATTCTGGATGCCTCCCTAGAGCTGTTCAAGTTCACCATTGGTAT GGGGGAGCTGGCTTTCCAGGAACAGCTGCGTTTTCGTGGGGTGGTCCTGCTGTTGCTGTTGGCCTACGTC CTTCTCACCTACGTCCTGCTGCTCAACATGCTCATTGCTCTCATGAGCGAAACTGTCAACCACGTTGCTG ACAACAGCTGGAGCATCTGGAAGTTGCAGAAAGCCATCTCTGTCTTGGAGATGGAGAATGGTTACTGGTG GTGCCGGAGGAAGAAACATCGTGAAGGGAGGCTGCTGAAAGTCGGCACCAGGGGGGATGGTACCCCTGAT GAGCGCTGGTGCTTCAGGGTGGAGGAAGTAAATTGGGTTGCTTGGGAGAAGACTCTTCCCACCTTATCTG AGGATCCATCAGGGCCAGGCATCACTGGTAATAAAAAGAACCCAACCTCTAAACCGGGGAAGAACAGTGC CTCAGAGGAAGACCATCTGCCCCTTCAGGTCCTCCAGTCCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217180 representing NM_001270798 Red=Cloning site Green=Tags(s) MTSASSPPAFRLETSDGDEEGNAEVNKGKQEPPPMESPFQREDRNSSPQIKVNLNFIKRPPKNTSAPSQQ EPDRFDRDRLFSVVSRGVPEELTGLLEYLRWNSKYLTDSAYTEGSTGKTCLMKAVLNLQDGVNACIMPLL QIDKDSGNPKPLVNAQCIDEFYQGHSALHIAIEKRSLQCVKLLVENGADVHLRACGRFFQKHQGTCFYFG ELPLSLAACTKQWDVVTYLLENPHQPASLEATDSLGNTVLHALVMIADNSPENSALVIHMYDGLLQMGAR LCPTVQLEEISNHQGLTPLKLAAKEGKIEIFRHILQREFSGPYQPLSRKFTEWCYGPVRVSLYDLSSVDS WEKNSVLEIIAFHCKSPNRHRMVVLEPLNKLLQEKWDRLVSRFFFNFACYLVYMFIFTVVAYHQPSLDQP AIPSSKATFGESMLLLGHILILLGGIYLLLGQLWYFWRRRLFIWISFMDSYFEILFLLQALLTVLSQVLR FMETEWYLPLLVLSLVLGWLNLLYYTRGFQHTGIYSVMIQKVILRDLLRFLLVYLVFLFGFAVALVSLSR EARSPKAPEDNNSTVTEQPTVGQEEEPAPYRSILDASLELFKFTIGMGELAFQEQLRFRGVVLLLLLAYV LLTYVLLLNMLIALMSETVNHVADNSWSIWKLQKAISVLEMENGYWWCRRKKHREGRLLKVGTRGDGTPD ERWCFRVEEVNWVAWEKTLPTLSEDPSGPGITGNKKNPTSKPGKNSASEEDHLPLQVLQSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270798
ORF Size	2283 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270798.1, NP_001257727.1
RefSeq Size	2750 bp
RefSeq ORF	2286 bp
Locus ID	29465
UniProt ID	Q9WUD2
MW	86.7 kDa
Gene Summary	ion channel that may be activated by high temperature; may transduce high threshold heat responses in sensory ganglia [RGD, Feb 2006]

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