Ldb3 (NM_001277165) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1564875; ZASP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217144 representing NM_001277165 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTACAGTGTGACTCTGACTGGGCCTGGGCCCTGGGGCTTCCGTCTGCAGGGAGGCAAGGACTTCA ATATGCCCCTCACTATCTCCCGGATCACCCCAGGCAGCAAGGCGGCCCAGTCCCAGCTCAGTCAAGGAGA TCTTGTGGTGGCCATTGATGGAGTCAACACAGACACCATGACCCACCTGGAGGCCCAGAACAAGATCAAG TCGGCCAGCTACAACCTGAGCCTCACTCTGCAGAAGTCCAAGCGTCCTATCCCCATCTCCACGACAGCAC CCCCTATCCAGTCCCCATTGCCAGTGATCCCCCACCAAAAGGACCCTTCTCTGGACACGAACAGCAGCCT GGCGACTCCCAGCCCCAGCCCTGAGGCGAGGGCCAGCCCAGGCACCTCAGGCACCCTGGAGTTCGGGGAC ACCTTTAGCTCTTCCTTCTCCCAGACCTCTGTCTGCACCTCACACATGGAGGCCTCGGGGCCTGTCCTTC CTCTGGGCAGCCCAGTGGCCAAGGCCAGCTCAGAGGGTACCCAGGGCTCAGTCAGCCCCAAAGTTCTGCC AGGCCCCAGCCAACCAAGGCTATATAACAACCCCATTGGCCTGTACTCAGCAGAGACCCTGAGGGAGATG GCTCAGATGTATCAGATGAGCCTCCGAGGGAAGGCCTCAGGTGCTGGACTCCTAGGAGGGAGCCTCCCTG TGAAAGACCTGGCGGTGGACAGCGCCTCTCCCGTGTACCAGGCTGTGATCAAAACCCAAAGCAAGCCAGA AGATGAGGCTGATGAGTGGGCCCGCCGGTCCTCCAACCTGCAGTCTCGTTCCTTCCGAATCTTGGCCCAG ATGACCGGGACAGAATACATGCAAGACCCTGATGAGGAAGCTCTGCGAAGGTCAAGCACCCCTATTGAGC ATGCTCCAGTGTGCACAAGCCAGGCCACTTCCCCGCTGCTGCCTGCTTCTGCCCCGTCGCCTGCTGCTGC CTCTCCCATTGCGGCCTCGCCAACCCTGGCCACAGCTGCTGCCGCCCATGCTGTTGCTGCCTCCGCTGCA GGCCCTGCCTCAAGCCCCGTGGAGAATCCGAGGCCACAGGCTTCTGCTTACAGCCCTGCAGCAGCCACTT CTCCAGCACCTGCTGCCCATACCAGCTACAGTGAAGGTCCAGCTGCCCCTGCACCCAAGCCTCGGGTTGT AACCACTGCCAGCATCCGGCCTTCGGTCTACCAGCCAGTGCCTGCATCTCCCTACAGCCCATCTCCAGGA GCCAATTATAGTCCGACTCCCTATACTCCTTCACCAGCTCCTGCTTACACCCCCTCACCTGCTCCCACCT ATACCCCCTCACCTGCACCCACCTACTCCCCCTCTCCTGCACCAGCTTATACCCCATCACCTGCCCCAAA CTATAACCCTACACCATCAGCTGCCTACAGTGGGGGCCCTTCAGAATCTGCCAGCCGCCCACCCTGGGTG ACCGACGACAGCTTCTCTCAGAAGTTTGCTCCCGGAAAGAGCACCACCTCTGTCAGCAAGCAGACCCTGC CCAGGGGGGCTCCAGCCTACAACCCAACAGGCCCACAAGTGACTCCTCTTGCCAGGGGCACCTTCCAGAG AGCTGAGCGCTTCCCAGCAAGCAGCCGGACTCCCCTCTGTGGGCACTGTAACAATGTCATCAGGGGTCCT TTTCTGGTAGCCATGGGCCGCTCTTGGCACCCAGAAGAGTTCAATTGTGCCTACTGCAAGACCTCCCTAG CAGACGTGTGCTTTGTGGAGGAGCAGAACAATGTTTACTGTGAGCGGTGTTACGAGCAGTTTTTTGCCCC GATCTGTGCCAAGTGTAACACCAAGATCATGGGGGAAGTGATGCACGCGCTGCGACAGACCTGGCATACC ACCTGCTTCATCTGCGCCGCCTGTAAGAAGCCTTTTGGGAACAGCCTCTTCCACATGGAGGATGGAGAGC CATACTGTGAGAAAGACTACATCAACCTGTTCAGCACGAAGTGTCATGGATGTGATTTCCCTGTGGAAGC TGGTGACAAGTTTATCGAGGCATTGGGGCACACCTGGCACGACACCTGCTTCATTTGTGCGGTCTGCCAC GTGAATCTGGAGGGGCAGCCATTCTACTCCAAGAAAGACAAACCGCTGTGCAAGAAGCACGCGCATGCCA TCAACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217144 representing NM_001277165 Red=Cloning site Green=Tags(s) MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIK SASYNLSLTLQKSKRPIPISTTAPPIQSPLPVIPHQKDPSLDTNSSLATPSPSPEARASPGTSGTLEFGD TFSSSFSQTSVCTSHMEASGPVLPLGSPVAKASSEGTQGSVSPKVLPGPSQPRLYNNPIGLYSAETLREM AQMYQMSLRGKASGAGLLGGSLPVKDLAVDSASPVYQAVIKTQSKPEDEADEWARRSSNLQSRSFRILAQ MTGTEYMQDPDEEALRRSSTPIEHAPVCTSQATSPLLPASAPSPAAASPIAASPTLATAAAAHAVAASAA GPASSPVENPRPQASAYSPAAATSPAPAAHTSYSEGPAAPAPKPRVVTTASIRPSVYQPVPASPYSPSPG ANYSPTPYTPSPAPAYTPSPAPTYTPSPAPTYSPSPAPAYTPSPAPNYNPTPSAAYSGGPSESASRPPWV TDDSFSQKFAPGKSTTSVSKQTLPRGAPAYNPTGPQVTPLARGTFQRAERFPASSRTPLCGHCNNVIRGP FLVAMGRSWHPEEFNCAYCKTSLADVCFVEEQNNVYCERCYEQFFAPICAKCNTKIMGEVMHALRQTWHT TCFICAACKKPFGNSLFHMEDGEPYCEKDYINLFSTKCHGCDFPVEAGDKFIEALGHTWHDTCFICAVCH VNLEGQPFYSKKDKPLCKKHAHAINV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277165
ORF Size	2178 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277165.1, NP_001264094.1
RefSeq Size	4847 bp
RefSeq ORF	2181 bp
Locus ID	498587
MW	76.8 kDa

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