Nrg1 (NM_001271128) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216722 representing NM_001271128 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAGCGCAAAGAAGGCAGAGGCAAGGGGAAGGGCAAGAAGAAGGACCGGGGATCCCGCGGGAAGC CCGGGCCCGCCGAGGGCGACCCGAGCCCAGCACTGCCTCCCAGATTGAAAGAAATGAAGAGCCAGGAGTC AGCTGCAGGCTCCAAGCTAGTGCTCCGGTGCGAAACCAGCTCCGAGTACTCCTCACTCAGATTCAAATGG TTCAAGAATGGGAACGAGCTGAACCGCAAAAATAAACCAGAAAACATCAAGATACAGAAGAAGCCAGGGA AGTCAGAGCTTCGAATTAACAAAGCATCCCTGGCTGACTCTGGAGAGTATATGTGCAAAGTGATCAGCAA GTTAGGAAATGACAGTGCCTCTGCCAACATCACCATTGTTGAGTCAAACGAGTTCATCACTGGCATGCCA GCCTCGACTGAGACAGCCTATGTGTCCTCAGAGTCTCCCATTAGAATCTCAGTTTCAACAGAAGGCGCAA ACACTTCTTCATCCACATCGACATCCACGACTGGGACCAGCCATCTCATAAAGTGCGCGGAGAAGGAGAA AACTTTCTGTGTGAATGGGGGCGAGTGCTTCACGGTGAAGGACCTGTCAAACCCGTCAAGATACTTGTGC AAGTGCCAACCTGGATTCACTGGAGCAAGATGTACTGAGAATGTACCCATGAAAGTCCAAACCCAAGAAA AAGCGGAGGAACTCTACCAGAAGAGGGTGCTGACAATTACTGGCATCTGTATCGCCCTGCTGGTGGTCGG CATCATGTGTGTGGTGGCCTACTGCAAAACCAAGAAGCAGCGGCAGAAGCTTCATGATCGGCTTCGGCAG AGTCTTCGGTCAGAACGGAGCAACCTGGTGAACATAGCGAATGGGCCTCACCACCCAAACCCACCGCCAG AGAACGTGCAGCTGGTGAATCAATACGTATCTAAAAACGTCATCTCCAGTGAGCATATTGTTGAGAGAGA AGTGGAGACTTCCTTTTCCACCAGTCATTACACTTCCACAGCCCATCACTCCACGACTGTCACCCAGACT CCTAGTCACAGCTGGAGTAATGGGCACACGGAGAGCATCATTTCAGAAAGCAACTCCGTAATCATGATGT CTTCGGTAGAGAACAGCAGGCACAGCAGTCCCGCCGGGGGCCCACGAGGACGTCTTCATGGCCTGGGAGG CCCTCGTGATAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGACTCCTACAGAGACTCTCCTCATAGC GAAAGACATAACCTTATAGCTGAGCTAAGGAGAAACAAGGCTTACAGATCCAAATGCATGCAGATCCAGC TGTCAGCAACTCATCTTAGACCCTCTTCCATTACCCATTTGGGCTTCATTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216722 representing NM_001271128 Red=Cloning site Green=Tags(s) MSERKEGRGKGKGKKKDRGSRGKPGPAEGDPSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKW FKNGNELNRKNKPENIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEFITGMP ASTETAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLIKCAEKEKTFCVNGGECFTVKDLSNPSRYLC KCQPGFTGARCTENVPMKVQTQEKAEELYQKRVLTITGICIALLVVGIMCVVAYCKTKKQRQKLHDRLRQ SLRSERSNLVNIANGPHHPNPPPENVQLVNQYVSKNVISSEHIVEREVETSFSTSHYTSTAHHSTTVTQT PSHSWSNGHTESIISESNSVIMMSSVENSRHSSPAGGPRGRLHGLGGPRDNSFLRHARETPDSYRDSPHS ERHNLIAELRRNKAYRSKCMQIQLSATHLRPSSITHLGFIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271128
ORF Size	1383 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271128.1, NP_001258057.1
RefSeq Size	3397 bp
RefSeq ORF	1386 bp
Locus ID	112400
MW	50.8 kDa
Gene Summary	ligand for Erbb3 and Erbb4 receptors; gene produces many different alternative splicing isoforms; involved in neural and organ development [RGD, Feb 2006]

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