Gck (NM_001270850) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GLK; GLUKA; RNGK2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216698 representing NM_001270850 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGATGACAGAGCCAGGATGGAGGCCACCAAGAAGGAAAAGGTCGAGCAGATCCTGGCAGAGTTCC AGCTGCAGGAGGAAGACCTGAAGAAGGTGATGAGCCGGATGCAGAAGGAGATGGACCGTGGCCTGAGGCT GGAGACCCACGAGGAGGCCAGTGTAAAGATGTTACCCACCTACGTGCGTTCCACCCCAGAAGGCTCAGAA GTCGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTTCAGAGTGATGCTGGTCAAAGTGGGAGAGG GGGAGGCAGGGCAGTGGAGCGTGAAGACAAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACGGG CACTGCCGAGATGATGAAGCACAAGAAACTGCCCCTGGGCTTCACCTTCTCCTTCCCTGTGAGGCACGAA GACCTAGACAAGGGCATCCTCCTCAATTGGACCAAGGGCTTCAAGGCCTCTGGAGCAGAAGGGAACAACA TCGTAGGACTTCTCCGAGATGCTATCAAGAGGAGAGGGGACTTTGAGATGGATGTGGTGGCAATGGTGAA CGACACAGTGGCCACAATGATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGGGC ACTGGCTGCAATGCCTGCTACATGGAGGAAATGCAGAATGTGGAGCTGGTGGAAGGGGATGAGGGACGCA TGTGCGTCAACACGGAGTGGGGCGCCTTCGGGGACTCGGGCGAGCTGGATGAGTTCCTACTGGAGTATGA CCGGATGGTGGATGAAAGCTCAGCGAACCCCGGTCAGCAGCTGTACGAGAAGATCATCGGTGGGAAGTAT ATGGGCGAGCTGGTACGACTTGTGCTGCTTAAGCTGGTGGACGAGAACCTTCTGTTCCACGGAGAGGCCT CGGAGCAGCTGCGCACGCGTGGTGCTTTTGAGACCCGTTTCGTGTCACAAGTGGAGAGCGACTCCGGGGA CCGAAAGCAGATCCACAACATCCTAAGCACTCTGGGGCTTCGACCCTCTGTCACCGACTGCGACATTGTG CGCCGTGCCTGTGAAAGCGTGTCCACTCGCGCCGCCCATATGTGCTCCGCAGGACTAGCTGGGGTCATAA ATCGCATGCGCGAAAGCCGCAGTGAGGACGTGATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAA GCTGCACCCGAGCTTCAAGGAGCGGTTTCACGCCAGTGTGCGCAGGCTGACACCCAACTGCGAAATCACC TTCATCGAATCAGAGGAGGGCAGCGGCAGGGGAGCCGCGCTGGTCTCTGCGGTGGCCTGCAAGAAGGCTT GCATGCTGGCCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216698 representing NM_001270850 Red=Cloning site Green=Tags(s) MLDDRARMEATKKEKVEQILAEFQLQEEDLKKVMSRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSE VGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQMYSIPEDAMTGTAEMMKHKKLPLGFTFSFPVRHE DLDKGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRQCEVGMIVG TGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRMVDESSANPGQQLYEKIIGGKY MGELVRLVLLKLVDENLLFHGEASEQLRTRGAFETRFVSQVESDSGDRKQIHNILSTLGLRPSVTDCDIV RRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEIT FIESEEGSGRGAALVSAVACKKACMLAQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270850
ORF Size	1344 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270850.1, NP_001257779.1
RefSeq Size	2220 bp
RefSeq ORF	1347 bp
Locus ID	24385
UniProt ID	P17712
MW	50.1 kDa
Gene Summary	catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006]

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