Tpm1 (NM_001034071) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Alpha-tm; Tma2; Tmsa
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216242 representing NM_001034071 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTCGACAAAGAGAACGCCTTGGATCGAGCAGAGC AGGCGGAGGCTGACAAGAAGGCTGCGGAAGACCGGAGCAAGCAGCTCGAGGAGGACATCTCAGCAAAAGA GAAGCTGCTGCGGGCGTCGGAGGACGAGCGGGACCGGGTGCTGGAGGAGCTGCACAAGGCAGAGGACAGC CTGCTGGCTGCCGACGAGACCGCCGCCAAGGCTGAAGCTGACGTAGCATCTCTGAACAGACGCATCCAGC TGGTTGAGGAGGAGTTGGATCGCGCTCAGGAGCGTCTGGCCACAGCTCTACAGAAGCTGGAGGAGGCTGA GAAGGCTGCAGATGAGAGTGAGAGAGGCATGAAAGTCATTGAAAGCCGAGCCCAAAAAGATGAAGAAAAG ATGGAGATTCAGGAGATCCAGCTGAAAGAGGCCAAGCACATTGCTGAAGATGCTGACCGAAAGTATGAAG AGGTGGCCCGTAAGCTGGTCATCATCGAGAGCGATCTGGAGCGTGCGGAGGAGAGGGCTGAGCTCTCGGA AGGCCAAGTTCGACAGCTGGAAGAACAGTTAAGAATAATGGATCAGACCTTGAAAGCATTAATGGCTGCA GAGGATAAGTACTCTCAGAAAGAAGACAAGTATGAAGAGGAGATCAAGGTTCTCTCTGACAAGCTGAAGG AGGCTGAGACCCGGGCTGAGTTTGCAGAGAGATCAGTAACCAAATTGGAGAAAAGCATTGATGACTTAGA AGAGAAAGTGGCACATGCCAAAGAAGAAAACCTTAGTATGCACCAGATGCTGGACCAGACTTTACTGGAG CTAAACAACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216242 representing NM_001034071 Red=Cloning site Green=Tags(s) MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEEDISAKEKLLRASEDERDRVLEELHKAEDS LLAADETAAKAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEK MEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGQVRQLEEQLRIMDQTLKALMAA EDKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEEKVAHAKEENLSMHQMLDQTLLE LNNM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001034071
ORF Size	852 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001034071.1, NP_001029243.1
RefSeq Size	1740 bp
RefSeq ORF	855 bp
Locus ID	24851
MW	32.8 kDa
Gene Summary	This gene is a member of the tropomyosin family and encodes a protein that heterodimerizes with a beta subunit, binding actin in both muscle and nonmuscle cells. In both smooth and striated muscle cells, it plays a role in calcium dependent-regulation of muscle contraction. In nonmuscle cells, it stabilizes cytoskeleton actin filaments. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

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