Ch25h (NM_001025415) Rat Tagged ORF Clone
CAT#: RR215344
- TrueORF®
Ch25h (Myc-DDK-tagged ORF) - Rat cholesterol 25-hydroxylase (Ch25h), (10 ug)
"NM_001025415" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR215344 representing NM_001025415
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGCCACAACGTTTCGGAGCTCCAAGACCTTGGTTGTTCCAACCAGCTGCTCCTGCAGCCTCTCT GGGACAGCATAAGGACGGGAGAGGCGTCCGCGCGCTCACCCTTCTTCCCAGTCATCTTTTCTATCTTCAC TTACCTGGGCTTCTGCCTACCGTTCGTGGTGCTAGATGTCCTCTGTCCCTGGGTGCCCATCTTGAGACGC TACAAGATCCACCCGGACTTCTCGCCGTCCGTAAGGCAGCTTCTGCCCTGCCTGGGGCTGACACTCTACC AGCACCTGGTGTTCGTGTTCCCAGTGACGCTGATGCACTGGGCGCGCAGCCCCGCGCTGCTGCCGCGGGA GGCCCCCGAGCTCTCCCAGCTCCTGAGTCACGTCCTGATCTGCCTGCTGCTCTTCGACACCGAGATCTTC GCGTGGCATCTGCTGCACCATAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCATCACCAGAACT CGTCCTCCTTCGCGTTGGCTACCCAATACATGAGCGTCTGGGAGCTGCTTTCTCTGACCTTCTTCGATGT GCTGAACGTCGCGATGCTTCAGTGTCACCCACTCACCATCCTCGTCTTTCACGTGGTTAACATCTGGCTG TCGGTGGAGGACCACTCAGGTTACGACTTTCCGTGGTCCACTCACAGACTTGTGCCGTTTGGCTGGTACG GGGGCGTGGCTCACCACGACCTGCATCACTCTCAGTTTAACTGCAACTTTGCTCCGTACTTCACACACTG GGACAAAATGCTGGGGACCCTGCGTTGCGCACCCCACTCGAAGCGCCTGTGTGCCGGCAGTGAGAGCTGC TTGGACTCCGGAGAGCAATGTACTGTACACTTGAACCAGAAGAAGAAACAGACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215344 representing NM_001025415
Red=Cloning site Green=Tags(s) MACHNVSELQDLGCSNQLLLQPLWDSIRTGEASARSPFFPVIFSIFTYLGFCLPFVVLDVLCPWVPILRR YKIHPDFSPSVRQLLPCLGLTLYQHLVFVFPVTLMHWARSPALLPREAPELSQLLSHVLICLLLFDTEIF AWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVNIWL SVEDHSGYDFPWSTHRLVPFGWYGGVAHHDLHHSQFNCNFAPYFTHWDKMLGTLRCAPHSKRLCAGSESC LDSGEQCTVHLNQKKKQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001025415 |
ORF Size | 894 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025415.1, NP_001020586.1 |
RefSeq Size | 1341 bp |
RefSeq ORF | 897 bp |
Locus ID | 309527 |
UniProt ID | Q4QQV7 |
MW | 34.4 kDa |
Gene Summary | Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN215344 | Ch25h (untagged ORF) - Rat cholesterol 25-hydroxylase (Ch25h), (10 ug) |
CNY 3,990.00 |
|
RR215344L3 | Lenti ORF clone of Ch25h (Myc-DDK-tagged ORF) - Rat cholesterol 25-hydroxylase (Ch25h), (10 ug) |
CNY 6,080.00 |
|
RR215344L4 | Lenti ORF clone of Ch25h (mGFP-tagged ORF) - Rat cholesterol 25-hydroxylase (Ch25h), (10 ug) |
CNY 6,650.00 |