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Polr2e (NM_001109614) Rat Tagged ORF Clone
CAT＃: RR213420
- TrueORF®
Polr2e (Myc-DDK-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug)
"NM_001109614" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213420 representing NM_001109614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACGAGGAGGAAACGTATCGGCTGTGGAAGATCCGCAAGACTATCATGCAGCTGTGTCATGACC GAGGCTACCTGGTGACCCAGGATGAGCTGGACCAGACACTTGAAGAGTTCAAGGCACAGTTTGGGGACAA GCCCAGCGAAGGACGGCCGAGGCGCACAGACCTCACCGTGCTGGTGGCCCACAACGATGATCCCACAGAC CAGATGTTTGTCTTCTTCCCAGAGGAGCCCAAGGTGGGCATCAAGACCATCAAGGTGTACTGCCAGCGCA TGCAGGAGGAGAACATCACACGGGCGCTGATCGTGGTGCAGCAGGGCATGACACCCTCCGCCAAGCAGTC CCTGGTAGACATGGCCCCTAAGTACGTGCTAGAACAGTTTCTACAGCAGGAGCTGCTCATCAATATCACA GAACATGAGCTAGTCCCTGAGCACGTGGTCATGACCAAGGAGGAGGTGACGGAACTGCTGGCGCGATACA AGCTCCGTGAGAGCCAGCTGCCCAGGATCCAGGCTGGGGACCCGGTGGCACGGTACTTCGGGATCAAGCG AGGGCAGGTTGTGAAGATTATCCGCCCCAGTGAGACAGCCGGCCGGTACATCACCTACCGCCTGGTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213420 representing NM_001109614
Red=Cloning site Green=Tags(s) MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTD QMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAPKYVLEQFLQQELLINIT EHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRYITYRLVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001109614 |
| ORF Size | 630 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001109614.1, NP_001103084.1 |
| RefSeq Size | 1082 bp |
| RefSeq ORF | 633 bp |
| Locus ID | 690966 |
| UniProt ID | B0BNE2 |
| MW | 24.6 kDa |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213420 | Polr2e (untagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug) |
CNY 3,990.00 |
|
| RR213420L3 | Lenti ORF clone of Polr2e (Myc-DDK-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug) |
CNY 6,080.00 |
|
| RR213420L4 | Lenti ORF clone of Polr2e (mGFP-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (Polr2e), (10 ug) |
CNY 6,650.00 |
