Disp2 (NM_001107759) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-AC-Myc-DDK
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213123 representing NM_001107759. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTCCCGAGGCAAGCCCAGAGAGAAGCTGCTCCCTCCACACCTGTCCCCTGGAAGACCCAACCGGG GCTCCGGTACCACCACCAACAGTTTCCACGCTCCAGGCTGTAGACCCAACCAGTCCGTTAACAGCTGGC CATTTTGCCTTTCCTCGAGCGCCTCAAGACTATCAAGAAGGCAGTTCGTTACTAGGATTTGGAGACCAA GCATCTCTATGTGCCCATGTCTCCAACCTCAACACTTCCATAGACCCCTCTCAGCATGATGGGGTCTGG AAGCCACCTTCTGTGCAGCGCCATGTGGTCAGTGTCAGGCAGGAACGCACTTTCCGGATGCCGAAGAGC TATTCTCATATGATTGCTGAGTGGCCAATGGCTGTGCTCCTGGGATGTCTGGCCTTCATCTTCCTCTGT ACCCTAGCTGGGTTGTTGGGAAGCCCACCGCTTGACTTTTCTGAGCCTTTACTGGGTTTTGAGCCTCGA GACACAGAGATCAACCGAAAGCTAGAAGTTTGGAAAGCCATGCAGGCCCTCACTGGCCCCAAGAATCTG TTTTCTCTTTCCCCAGATCCTGAGGTGAACAGCTCAACCCTCCTCAGCACCCTGAGCCCTGCAGCCTGG GGCAGGGCCGAGGAAAGTGTGGTCCGGACCAAGAGGATGGTGGGGCCTGTGGAAGTCAAAGAAGAGGAG AACTTCTTTTGTGGTCCCCCTGAGAAAAAACTTGCAAAACTGGTGTTCGTGTCCACCTCTGGGGGAAGC CTGTGGAACCTGCAAGCCATCCATTCCATGTGTCGCATAGAACAGGAGCAGATCCGCTCTCACATCAGC TTTGGGGCTCTGTGCCAGCGATCAGCAGCCAATGAGTGCTGCCCCAGCTGGTCCCTGGGAAACTATCTG GCCGTGCTGTCTAACCGCTCCTCCTGCCAAGACACCACCCAAGCTGATACAGACCGCACACTGGCCCTG CTACGGTTCTGTGCCACCTTCTACCACCGTGGTGTCCTGGTGCCTGCTTGTGTGGGATCTGGGCAGGAC AAACCCCCATTTTGTGCCCAAGTTCCCGCCAAGTGTACCGGAAGCAGCGCTGTCTATCAACTCCTGCAC TTCCTGCTAGACAGAGACTTTCTGAGTCCCCAGACTGCGGATTACCAGGTGCCCTCCCTCAAGTTCGCC CTGCTCTTCCTGCCCATTATAAAGACCTCCTCTCTTCTAGACATCTACCTGGACGGCCTAGGTGACCCG ATTAAAGTTTCTGATAACTACACATCTATCAGTGGCATGGACTTGGGCCTCAAGCCCAGACTATTGAAG TACTACCTAGCAGAAGATACCATGTACCCCTTGCTATCCCTGGTTATCATCTTCTTTGGCATGTCCCTC TACCTGCGTTCACTCTTCATCACATTCATGTCACTTCTAGGGGTGCTGGGCTCCCTAATGGTGGCTTTC TTTCTTTACCATGTGGCATTCCGCATGGCCTACTTCCCCTTTGTCAATCTAGCGTCTCTCCTTCTGCTT AGTGGCGTCTGTGTCAATTACACACTCATCTTCTTCGATTTGTGGCGCCTCAGCAGGGGCCAGGTGCCC TCCGGGGGCCTAGCACACCGTGTGGGCCGTACCATGCACCACTTTGGCTACCTGCTCCTGGTCTCAGGC CTCACCACCAGCGCGGCCTTCTACGGCAGCTACCTGAGCCGCCTGCCCACAGTGCGTTGTTTTGCTCTT TTCATGGGCACCGCTGTTCTAGTGCATATGGGACTCACACTGCTCTGGCTTCCTGCCACCGTGGTGCTC CACGAGCGCTACCTGGCACACGGCTGTGTGGCCCAAGCGCAGGGCCAGAGGGGTGGCAGCGACCCCCTG CGGCTGTTGCTGGCGTTGCACAGGCGGATTCGCATTCTTCGCAAGATTTTTTCCATCCTCTCACGCCTG CTCTTTCAACGCCTGCTGCCCTGTGGTGTCATTAAGTTTCGATACATCTGGATCTGCTGGTTCGCGGCG TTGGCGGCAGGGGGCGCCTACATCGGCGGCGTCAGCCCTCGCCTGCGACTGCCCATTCTACTGCCACTT GGCGGCCAGTTCTTCCGGTCTAGCCATCCCTTTGAGCGCTTTGATGCAGAGTACCGCCAGCAGTTCCTG TTCGAAGACCTACCTCCAAATGAGGGCGGCAACTTGCCAGTGGTTCTGGTGTGGGGAATCCTGCCAGTG GACACTAGTGATCCCCTGGACCCTCGCACCAACAGCTCAGTGGTAAGCGATCCTGACTTCTCGCCCAGC AGCCCCGAGGCCCAGGAGTGGCTCCAGGCTCTCTGTCAGGGAGCCCAGAACCAGAGCTTCTTTGGAGAC CAGCCAGAGGGTTGGCCTACGCTGTGTTTAGTGGAAGCCCTGCAGCAGTGGATGGAAAGCCCCAGCTGT GGCCGCCTGGGTGCCGATCTATGCTGTGGCCAGTCAGAATTCCCCTGGGCCCCCCAGCTTTACCTGCAC TGTCTGAAGATGATGGCTCTGGAGCAAAGTCCTGATGGCACACGTGACCTGGGACTCCGCTTCGATTCT CACGGCAACCTAGCAGCTCTAGTTCTGAAGTTCCAGACCAACTTACCATACAGTACGGAGTATGGCCCA GTCCACCATTTCTACACTGAAGTCAGCCGCTGGCTGTCAACAGAGATGAGCAAGGCACCTCCTGGGCTC AACAAGGGTTGGTTCACCAGCACCTTGGAGCTGTACAGCTTACAGCATAGTCTAAGCACAGAGCCTGCC GTGGTGCTCGGTCTGGCTCTGGCACTAGCCTTTGCCACACTGTTGCTGGGCACTTGGAACGTTCCCCTC AGCCTGTTCTCTGTGGCAGCCGTGGCTGGCACTGTGCTGCTCACTGTGGGCTTGCTGGTTCTTCTTGAG TGGCAACTCAACACTGCCGAGGCCCTCTTTCTCTCTGCCTCTGTGGGCCTCTCTGTAGACTTAACTGTT AACTACTGTATCTCCTATCACTTGTGCCCCCACCCAGACCGCCTGAGCCGTGTGGCCTTCTCCTTACGT CAGACCAGTCGGGCCACAGCAATGGGGACTGGAGTGTTGTTTGCCTCTGGCGTGATCATGCTGCCTTCC ACCATACTGCTCTATCGAAAGCTGGGCATCATCGTCATGATGGTCAAGTTTCTTGGCTGTGGCTTTGCC AGCTTCTTCTTCCAGTCCCTGTGCTGTTTCTTCGGGCCAGAGAAGAATTGTGGGCAGATCCTATGGCCC TGTGCCCATCTGCCGTGGGATGCTGGGACTGAGGATCCCGATGAGAAGGGGCGAACAGGGCCACCAGGG TTCTCTGAACACTATGAGTTGCAGCCCCTGGCGCGGCGCCGGAGTCCCAGCTTCGACACCAGCACAGCC ACCAGCAAGCTTTCCCATCGGCCTTCCATACTCTCTGAAGACCTGCAGATACACGATGGCAGCTGCTGC CTCCAGCACGCCCAAGTCCCTGTCTCCCCAAGGGATCTGCTCCTGGACCACCAGACAGTCTTCAGCCAG TGTCCAGCCCTGCAGACCTCTTCTCCATATAAGCAGGTTGGTCCTAACCCCCAAACCTGGATCAGGCAA GATTCCCAGGGACAGAAAACTGAGCCCTTGCAGGCCCTGCCAGAAGGCCCTGCCCACTGCCCTAAGCCC AAAGTCATGGAAGAGCTCCCTGATGGCCTGTGCTCCTCAGCCAGCACCCTGGAGGGACTCAGCGTCTCA GATGACACCTGTGCCTCTGAGCCCAGTGTCCGTGTGCCAGATTCTGTGGGCACCTCCCCAGAAGTCATG AATGGCACTGGGCACCCCATGCTTGAGCGGGGTCAGCTGAATGGGAAACGTGACACCCTCTGGCTGGCA CTGAAGGAGACCATCTATGACCCAAACATGCCCAATTCTCACCACAGCAGCTTGTCCTGGAAGGGCCGT GGAGGGCCAGGTGATATAAGCCCCGTGGTACTTCCCAACAGTCAGCCAGATCTTCCAGATGTTTGGCTC CGTAGGCCTAGCACCTACGCCTCTGGCTACAGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RR213123 Blue=ORF Red=Cloning site Green=Tag(s) MAPEASPERSCSLHTCPLEDPTGAPVPPPTVSTLQAVDPTSPLTAGHFAFPRAPQDYQEGSSLLGFGDQ ASLCAHVSNLNTSIDPSQHDGVWKPPSVQRHVVSVRQERTFRMPKSYSHMIAEWPMAVLLGCLAFIFLC TLAGLLGSPPLDFSEPLLGFEPRDTEINRKLEVWKAMQALTGPKNLFSLSPDPEVNSSTLLSTLSPAAW GRAEESVVRTKRMVGPVEVKEEENFFCGPPEKKLAKLVFVSTSGGSLWNLQAIHSMCRIEQEQIRSHIS FGALCQRSAANECCPSWSLGNYLAVLSNRSSCQDTTQADTDRTLALLRFCATFYHRGVLVPACVGSGQD KPPFCAQVPAKCTGSSAVYQLLHFLLDRDFLSPQTADYQVPSLKFALLFLPIIKTSSLLDIYLDGLGDP IKVSDNYTSISGMDLGLKPRLLKYYLAEDTMYPLLSLVIIFFGMSLYLRSLFITFMSLLGVLGSLMVAF FLYHVAFRMAYFPFVNLASLLLLSGVCVNYTLIFFDLWRLSRGQVPSGGLAHRVGRTMHHFGYLLLVSG LTTSAAFYGSYLSRLPTVRCFALFMGTAVLVHMGLTLLWLPATVVLHERYLAHGCVAQAQGQRGGSDPL RLLLALHRRIRILRKIFSILSRLLFQRLLPCGVIKFRYIWICWFAALAAGGAYIGGVSPRLRLPILLPL GGQFFRSSHPFERFDAEYRQQFLFEDLPPNEGGNLPVVLVWGILPVDTSDPLDPRTNSSVVSDPDFSPS SPEAQEWLQALCQGAQNQSFFGDQPEGWPTLCLVEALQQWMESPSCGRLGADLCCGQSEFPWAPQLYLH CLKMMALEQSPDGTRDLGLRFDSHGNLAALVLKFQTNLPYSTEYGPVHHFYTEVSRWLSTEMSKAPPGL NKGWFTSTLELYSLQHSLSTEPAVVLGLALALAFATLLLGTWNVPLSLFSVAAVAGTVLLTVGLLVLLE WQLNTAEALFLSASVGLSVDLTVNYCISYHLCPHPDRLSRVAFSLRQTSRATAMGTGVLFASGVIMLPS TILLYRKLGIIVMMVKFLGCGFASFFFQSLCCFFGPEKNCGQILWPCAHLPWDAGTEDPDEKGRTGPPG FSEHYELQPLARRRSPSFDTSTATSKLSHRPSILSEDLQIHDGSCCLQHAQVPVSPRDLLLDHQTVFSQ CPALQTSSPYKQVGPNPQTWIRQDSQGQKTEPLQALPEGPAHCPKPKVMEELPDGLCSSASTLEGLSVS DDTCASEPSVRVPDSVGTSPEVMNGTGHPMLERGQLNGKRDTLWLALKETIYDPNMPNSHHSSLSWKGR GGPGDISPVVLPNSQPDLPDVWLRRPSTYASGYSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107759
ORF Size	4038 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107759.2, NP_001101229.2
RefSeq Size	6549 bp
RefSeq ORF	4041 bp
Locus ID	311324
MW	147.9 kDa

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