Zmynd8 (NM_001100838) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Prkcbp1; spikar
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212873 representing NM_001100838 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATCTCTACTCGCTCCAAAGATCCTGGCTCTACCGAGAGAACGGCCCAGAAACGGAAGGTCCCCA GCCCCCCACATTCCTCCAATGGCCATTCGCCCCAGGACTCATCCACAAGCCCCATTAAAAAGAAAAAGAA ACCCGGCTTACTGAACAGTAGCAGTAAGGATCAGTCAGAGCTAAGACATGGTCCGTTTTACTATATGAAG CAGCCACTCACCACAGACCCTGTTGATGTTGTACCGCAGGACGGACGGAATGACTTCTATTGCTGGGTTT GTCACCGGGAAGGACAAGTCCTTTGCTGTGAGCTCTGTCCCCGGGTTTATCACGCTAAGTGTCTGAGACT GACATCGGAACCAGAGGGGGACTGGTTTTGTCCTGAATGTGAGAAGATTACAGTAGCAGAATGCATCGAG ACGCAGAGCAAAGCCATGACCATGCTGACCATCGAACAGCTGTCCTACCTGCTCAAGTTTGCTATTCAGA AAATGAAGCAGCCCGGGACGGACGCATTCCAGAAGCCCGTTCCGTTGGAACAGCACCCCGACTATGCAGA GTATATTTTCCATCCCATGGATCTTTGCACGTTGGAAAAGAATGCAAAAAAGAAGATGTATGGCTGTACG GAAGCCTTCCTGGCTGATGCCAAGTGGATCCTGCACAACTGCATTATTTATAATGGGGGAAACCACAAGT TGACGCAAATAGCAAAGGTTGTCATCAAGATCTGTGAGCACGAGATGAATGAAATTGAAGTATGCCCAGA ATGTTATCTCGCAGCTTGCCAGAAACGAGACAACTGGTTCTGTGAGCCCTGTAGCAATCCGCACCCTTTG GTCTGGGCAAAACTTAAAGGATTCCCATTCTGGCCAGCGAAAGCTCTGAGGGACAAAGACGGACAGGTTG ATGCCCGTTTCTTTGGACAACATGACAGAGCCTGGGTTCCAGTCAATAATTGCTACCTCATGTCAAAAGA AATCCCCTTTTCTGTGAAAAAGACTAAGAGTATCTTCAACAGCGCCATGCAGGAGATGGAAGTTTACGTG GAGAACATACGGAAGAAGTTCGGGGTTTTTAATTACTCCCCGTTCAGGACGCCCTACACCCCCAATAGCC AGTACCAAATGCTGCTGGATCCCAGCAATCCCAGCGCCGGCACTGCCAAGACAGACAAACAGGAGAAGGT GAAGCTCAATTTTGACATGACGGCGTCCCCCAAGATCATTCTGAGCAAGCCCCTTCTGAGCGGGGGCGCG GGCCGCAGGATCTCCCTGTCCGACATGCCTCGCTCCCCCACCAGCACGAACTCCTCTGTGCACACGGGCT CCGATGTGGAGCAGGACCCCGAGAAGAAGGCCCCATCCAGCCACTTCAGCGCAAGCGAAGAGTCCATGGA CTTCCTTGATAAGAGCACAGCGTCTCCAGCCTCCACCAAGACGGGGCAAGCAGGGAGCTTGTCCGGCAGC CCGAAGCCCTTCTCTCCACAAGCGCCAACGCCCATCATGACGAAACCTGACAAGACCTCCACCGCCACCA CCGGGAGCATCCTGAACTTGAACCTGGATCGAAGCAAAGCCGAGATGGACCTGAAGGAGCTGAGCGAGTC GGTCCAGCAGCAGTCAGCTCCCGTCCCCCTCATCTCTCCCAAGCGGCAAATTCGAAGCCGTTTCCAGCTC AACCTGGACAAGACCATAGAGAGTTGCAAAGCACAGCTAGGCATAAACGAGATCTCGGAGGACGTTTATA CGGCCGTGGAGCACAGCGACTCAGAGGATTCGGAAAAGTCAGAGAGCAGCGACAGCGAGTACGTCAGCGA CGAGGAACAGAAGCCCAAGAATGAGCCGGAGGACCCCGAGGACAAGGAGGGGAGCCGGGTGGACAAAGAG GCCCCTGCCATCAAAAGGAAGCCCAAGCCCTCGAACCAGGTGGAGGTCAAAGAGGAAGTGAAGAGTAACT CGCCCGTTGGAGAGAAAGTGGACCCCGCACCCGCCAAGGACAAGGCCAGCCCCGAGCCCGAGAAGGACTT TGCAGAGAAAGCAAAGCCATCCCCTCACCCCACGAAGGACAAATTGAAGGGAAAGGATGAGACAGATTCT CCCACAGTGCACTTGGGCTTGGATTCGGACTCAGAGAGCGAACTTGTCATAGACTTAGGAGAAGATCCTT CTGGGCGGGAGGGTCGAAAAAACAAGAAAGAGCCCAAGGTGCCGTCGCCCAAGCAGGATGCCATAGGTAA ACCGCCACCACCCACTCCGGCCGGCAACCAGTCTCCCCCAGAGACGCCGGTTCTCACCCGCTCAGCCACG CAAGCACCTGCGGCCGGGGTCACCGTGGCTGCCACCACCAGCACGATGTCTACCGTCACGGTCACAGCGC CGGCCACCGCCGCCGTCACCGGAAGCCCAGTGAAGAAGCAGAGGCCGCTCTTACCGAAGGAGACTGTCCC AGCCGTGCAGCGGGTCGTGTGGAACGCATCAAGTAAGTTTCAAACGTCCTCCCAAAAGTGGCACATGCAG AAGATACAGCGCCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGAGCCAACAGCAGCAGCCTC AGTCTTCCCAGGGGACAAGATATCAGACCAGACAGGCCGTGAAAGCTGTCCAGCAGAAGGAGGTCACCCA GAGCCCGTCCACATCCACCATCACGCTGGTGACCAGCACACAGCCGGCAGCCCTGGTCAGCAGCTCGGGC TCTGCCAGTACCTTAGCGTCTGTGATCAACGCTGACCTGCCCATTGCCACTGCCTCAGCCGATGTGGCTG CGGACATTGCCAAGTACACCAGCAAAATGATGGACGCCATAAAGGGGACTATGACAGAAATCTACAACGA CCTCTCCAAGAACACCACTGGGAGCACAATTGCTGAGATTCGAAGGCTGAGGATTGAGATTGAGAAGCTG CAGTGGCTGCACCAGCAGGAGCTCGCCGAGATGAAGCACAACTTGGAGTTGACCATGGCAGAGATGCGGC AGAGCTTGGAACAGGAGCGGGATCGGCTCATCGCCGAGGTGAAGAAGCAGCTGGAGCTGGAGAAGCAGCA GGCGGTGGACGAGACCAAGAAGAAGCAGTGGTGTGCCAACTGCAAGAAGGAGGCCATTTTTTACTGCTGC TGGAACACCAGCTACTGCGACTACCCCTGTCAGCAGGCCCACTGGCCCGAGCACATGAAGTCCTGTACCC AGTCGGCTACCGCCCCTCAGCAGGAAGCGGATGCTGAGGCAAACCAGGAAACAGGAAATAAGACATCTCA GGGCAGCTCTTCCAATACACAGTCAGCACCTTCAGAACCAGCCAGCACCCCCAAAGAGAAAGAGACGTCG TCATCGTCCTCGAATTCCTCCTCAACGGAGAAGAGCAAGGACAGTAACTCGACCCTGGACCTTTCCGGCT CCAGAGAGACGCCCTCCTCCATTCTCTTAGGCTCCAACCAAGGCTCTGACCATTCCCGGAGCAGCAAGGC GGGTTTGTGGAGCAGCAGCGAGGAAAAGCGAGGGTCATCCCGCTCCGAGCACAGTGCAGGGACCAGCACG AAGAACCTCATGCCCAAAGAGTCCCGGGAGTCTAGGCTAGACACCTTCTGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212873 representing NM_001100838 Red=Cloning site Green=Tags(s) MDISTRSKDPGSTERTAQKRKVPSPPHSSNGHSPQDSSTSPIKKKKKPGLLNSSSKDQSELRHGPFYYMK QPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLRLTSEPEGDWFCPECEKITVAECIE TQSKAMTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYAEYIFHPMDLCTLEKNAKKKMYGCT EAFLADAKWILHNCIIYNGGNHKLTQIAKVVIKICEHEMNEIEVCPECYLAACQKRDNWFCEPCSNPHPL VWAKLKGFPFWPAKALRDKDGQVDARFFGQHDRAWVPVNNCYLMSKEIPFSVKKTKSIFNSAMQEMEVYV ENIRKKFGVFNYSPFRTPYTPNSQYQMLLDPSNPSAGTAKTDKQEKVKLNFDMTASPKIILSKPLLSGGA GRRISLSDMPRSPTSTNSSVHTGSDVEQDPEKKAPSSHFSASEESMDFLDKSTASPASTKTGQAGSLSGS PKPFSPQAPTPIMTKPDKTSTATTGSILNLNLDRSKAEMDLKELSESVQQQSAPVPLISPKRQIRSRFQL NLDKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSESSDSEYVSDEEQKPKNEPEDPEDKEGSRVDKE APAIKRKPKPSNQVEVKEEVKSNSPVGEKVDPAPAKDKASPEPEKDFAEKAKPSPHPTKDKLKGKDETDS PTVHLGLDSDSESELVIDLGEDPSGREGRKNKKEPKVPSPKQDAIGKPPPPTPAGNQSPPETPVLTRSAT QAPAAGVTVAATTSTMSTVTVTAPATAAVTGSPVKKQRPLLPKETVPAVQRVVWNASSKFQTSSQKWHMQ KIQRQQQQQQQQQQQQQSQQQQPQSSQGTRYQTRQAVKAVQQKEVTQSPSTSTITLVTSTQPAALVSSSG SASTLASVINADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKL QWLHQQELAEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCC WNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEANQETGNKTSQGSSSNTQSAPSEPASTPKEKETS SSSSNSSSTEKSKDSNSTLDLSGSRETPSSILLGSNQGSDHSRSSKAGLWSSSEEKRGSSRSEHSAGTST KNLMPKESRESRLDTFWD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100838
ORF Size	3624 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100838.1, NP_001094308.1
RefSeq Size	4019 bp
RefSeq ORF	3627 bp
Locus ID	296374
MW	133.6 kDa

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