Cps1 (NM_017072) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	cps; cps-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212123 representing NM_017072 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGAGGATTTTGACAGCATGCAAAGTGGTGAAGACACTGAAGAGTGGCTTTGGTTTGGCCAATGTGA CTTCGAAGCGACAGTGGGACTTCTCTAGACCTGGCATCAGGCTCCTTTCTGTGAAGGCACAGACAGCACA CATTGTTCTGGAAGATGGAACTAAGATGAAGGGCTACTCCTTTGGCCATCCCTCCTCGGTTGCTGGCGAA GTGGTTTTTAATACTGGCTTAGGAGGGTACTCGGAAGCACTTACTGATCCTGCCTACAAGGGGCAGATCC TCACCATGGCCAACCCTATCATTGGGAATGGTGGGGCCCCTGATACAACGGCACGTGATGAACTGGGACT GAATAAGTACATGGAGTCTGATGGAATCAAGGTGGCGGGTCTGCTGGTGCTGAATTACAGTCATGACTAC AACCACTGGCTGGCCACCAAGAGTCTGGGTCAGTGGCTGCAGGAGGAGAAGGTCCCTGCAATTTATGGAG TGGATACAAGAATGCTGACTAAAATAATTCGGGATAAGGGTACCATGCTTGGGAAGATTGAGTTTGAGGG CCAGTCTGTGGACTTTGTGGATCCTAATAAGCAGAATTTGATTGCCGAGGTTTCAACCAAGGATGTCAAG GTGTTTGGCAAAGGAAACCCCACGAAAGTGGTAGCCGTGGACTGTGGGATAAAAAACAATGTCATCCGCC TGCTAGTTAAGCGAGGAGCGGAAGTGCATTTGGTCCCCTGGAATCATGACTTCACCCAGATGGACTATGA CGGACTTCTGATCGCTGGAGGACCTGGGAACCCAGCTCTGGCACAGCCACTAATTCAGAACGTGAAGAAG ATTTTGGAGAGTGACCGCAAAGAGCCGTTGTTTGGAATCAGTACAGGAAACATAATAACAGGATTGGCTG CTGGCGCCAAATCCTACAAGATGTCCATGGCCAACAGAGGACAGAACCAACCTGTTTTGAATATCACAAA CAGACAGGCTTTCATAACTGCTCAGAATCATGGCTATGCTCTGGACAACACCCTCCCTGCTGGCTGGAAA CCACTGTTTGTCAATGTCAATGACCAAACAAATGAGGGGATTATGCATGAGAGCAAACCCTTCTTCGCTG TGCAGTTCCACCCAGAGGTCAGCCCGGGGCCAACAGACACTGAGTACCTATTTGATTCCTTCTTCTCGCT GATAAAGAAGGGCAAAGGCACCACCATTACCTCAGTTCTGCCCAAGCCAGCATTGGTGGCATCTCGAGTC GAGGTTTCCAAGGTCCTTATCCTAGGATCAGGAGGCCTGTCCATTGGTCAGGCAGGTGAATTTGATTACT CCGGATCTCAGGCTGTAAAAGCCATGAAGGAAGAAAACGTCAAAACAGTCCTGATGAACCCGAACATTGC ATCCGTGCAGACCAACGAGGTGGGATTGAAGCAGGCAGATGCAGTCTACTTTCTCCCTATCACCCCCCAG TTTGTCACAGAAGTTATCAAGGCTGAACGGCCCGATGGGTTAATTCTGGGCATGGGTGGCCAGACAGCTC TGAACTGTGGAGTGGAACTATTCAAGAGGGGTGTGCTCAAGGAATATGGTGTGAAGGTCTTGGGAACATC GGTCGAGTCCATTATGGCCACAGAAGACAGGCAGCTTTTCTCAGACAAGCTGAATGAGATCAACGAGAAG ATTGCTCCTAGCTTTGCAGTGGAATCAATGGAGGATGCCTTGAAGGCAGCAGACACCATTGGCTACCCTG TGATGATTCGGTCTGCGTATGCTCTGGGTGGGTTAGGCTCCGGCATCTGTCCCAACAAGGAGACCTTAAT GGATCTTGGCACAAAGGCATTTGCTATGACCAACCAGATCCTGGTGGAGCGGTCAGTGACAGGTTGGAAA GAAATAGAATATGAAGTTGTCCGAGATGCGGATGATAACTGTGTCACAGTCTGTAACATGGAGAATGTTG ACGCCATGGGTGTTCACACAGGTGATTCAGTTGTTGTGGCCCCGGCCCAGACCCTCTCCAATGCAGAGTT CCAGATGTTGAGACGCACTTCAATCAATGTTGTTCGCCACTTGGGCATTGTGGGTGAATGCAACATTCAG TTTGCTCTTCACCCCACTTCCATGGAATACTGTATCATTGAAGTGAATGCCAGGCTCTCCAGGAGTTCTG CCCTGGCCTCCAAGGCCACTGGCTACCCACTGGCGTTCATCGCAGCAAAGATCGCTCTAGGAATCCCACT TCCAGAAATCAAGAATGTTGTGTCTGGGAAGACCTCAGCCTGCTTCGAACCTAGCCTGGATTACATGGTG ACCAAGATTCCTCGCTGGGACCTTGACCGTTTTCATGGAACATCCAGCCGAATTGGTAGCTCAATGAAAA GTGTAGGCGAGGTCATGGCCATTGGTCGCACCTTTGAGGAAAGTTTCCAGAAGGCTCTGAGGATGTGCCA TCCATCTGTGGATGGGTTCACTCCCCGTCTCCCAATGAATAAGGAATGGCCAGCAAACCTGGATCTGAGG AAGGAGCTGTCTGAACCCTCCAGCACCCGCATCTATGCCATTGCTAAGGCCTTGGAAAACAACATGTCCC TTGACGAAATCGTGAAGCTCACATCCATTGACAAGTGGTTTTTGTATAAGATGCGTGACATTTTAAACAT GGATAAGACACTGAAAGGGCTTAACAGTGAGTCTGTTACAGAAGAAACTCTGAGACAGGCCAAAGAGATT GGGTTCTCAGACAAGCAGATTTCAAAATGTTTAGGACTGACCGAGGCTCAGACAAGAGAGCTGAGATTGA AGAAAAACATCCACCCTTGGGTTAAACAGATTGATACATTGGCTGCAGAATACCCATCAGTGACAAATTA CCTGTATGTTACCTACAATGGCCAGGAGCACGACATCAAATTTGATGAACATGGAATAATGGTGCTGGGC TGTGGCCCATACCACATTGGCAGCAGTGTGGAATTTGATTGGTGTGCTGTCTCCAGTATCCGCACACTGC GCCAACTTGGCAAGAAGACGGTGGTGGTGAATTGTAACCCGGAGACTGTGAGCACTGACTTTGATGAGTG TGACAAACTCTACTTTGAAGAGCTGTCTTTGGAGAGAATCCTAGATATCTACCACCAGGAGGCATGTAAT GGCTGCATCATATCAGTCGGGGGCCAGATTCCAAACAACTTGGCGGTTCCGCTATACAAGAACGGTGTCA AGATCATGGGTACCAGTCCTCTGCAGATCGATAGGGCTGAGGATCGCTCCATCTTCTCGGCTGTCTTAGA TGAGCTGAAGGTGGCCCAGGCTCCTTGGAAAGCTGTTAACACTTTGAACGAGGCGCTGGAATTTGCAAAC TCTGTGGGCTATCCCTGCTTACTGAGACCTTCCTATGTTTTGAGTGGGTCTGCCATGAACGTGGTATTCT CTGAGGATGAGATGAAAAGGTTCCTTGAGGAGGCCACTCGAGTCTCTCAGGAACACCCAGTGGTGCTGAC CAAGTTTATTGAGGGGGCTCGGGAAGTGGAGATGGACGCTGTTGGCAAAGAAGGACGGGTCATCTCCCAT GCCATCTCTGAACATGTTGAAGATGCAGGTGTCCACTCAGGGGATGCCACACTGATGCTACCTACGCAGA CCATCAGCCAAGGAGCCATTGAAAAGGTGAAGGATGCCACACGAAAGATTGCAAAGGCTTTTGCCATCTC TGGGCCATTCAATGTTCAGTTTCTTGTCAAAGGAAATGATGTCTTGGTGATTGAGTGCAATCTGAGAGCC TCTCGATCCTTCCCCTTTGTCTCCAAGACTCTTGGGGTGGACTTCATTGATGTGGCCACCAAGGTGATGA TCGGAGAGAGTGTTGATGAGAAGCATCTACCCACACTGGAACAACCCATCATCCCCTCTGACTATGTTGC CATTAAGGCTCCCATGTTTTCCTGGCCCCGACTGAGGGATGCTGATCCTATTCTGAGATGTGAGATGGCT TCTACTGGAGAGGTGGCGTGTTTTGGTGAGGGCATTCATACAGCCTTCCTAAAGGCAATGCTGTCCACAG GGTTTAAGATACCTCAGAAGGGCATTCTGATTGGCATCCAGCAATCATTCCGTCCAAGATTCCTTGGTGT TGCTGAGCAGTTACACAATGAAGGTTTCAAGCTTTTTGCCACAGAAGCCACATCAGACTGGCTCAACGCC AACAATGTTCCTGCCACCCCAGTGGCTTGGCCATCTCAGGAAGGACAGAATCCCAGCCTCTCTTCCATCA GAAAGTTGATAAGAGACGGAAGCATTGACCTAGTGATTAACCTCCCCAATAACAACACCAAATTTGTCCA TGATAATTATGTGATTCGGAGGACAGCTGTGGACAGTGGAATTGCTCTGCTCACCAATTTCCAGGTGACC AAACTTTTTGCTGAGGCTGTGCAGAAAGCTCGTACTGTGGACTCCAAGAGTCTGTTCCACTACAGGCAGT ACAGCGCTGGAAAGGCAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212123 representing NM_017072 Red=Cloning site Green=Tags(s) MTRILTACKVVKTLKSGFGLANVTSKRQWDFSRPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGE VVFNTGLGGYSEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSHDY NHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVK VFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMDYDGLLIAGGPGNPALAQPLIQNVKK ILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWK PLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRV EVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQ FVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEK IAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLMDLGTKAFAMTNQILVERSVTGWK EIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSINVVRHLGIVGECNIQ FALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMV TKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLR KELSEPSSTRIYAIAKALENNMSLDEIVKLTSIDKWFLYKMRDILNMDKTLKGLNSESVTEETLRQAKEI GFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLG CGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACN GCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAN SVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFIEGAREVEMDAVGKEGRVISH AISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRA SRSFPFVSKTLGVDFIDVATKVMIGESVDEKHLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMA STGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNA NNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVT KLFAEAVQKARTVDSKSLFHYRQYSAGKAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017072
ORF Size	4500 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017072.2, NP_058768.1
RefSeq Size	4503 bp
RefSeq ORF	4503 bp
Locus ID	497840
UniProt ID	P07756
MW	164.6 kDa
Gene Summary	catalyzes synthesis of carbamyl phosphate from NH3, HCO3, and 2ATP [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.