Adcy8 (NM_017142) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ac8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210573 representing NM_017142 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTCTCGGATGTGCACTGCCTTAGCGGCAGCGAGGAACTCTATACCATTCACCCGACGCCCCCGG CCGCGGACGGCGGGAGCGGCTCTCGGCCGCAGCGGCTGCTGTGGCAGACGGCGGTGCGGCACATCACCGA GCAGCGCTTCATCCACGGCCACCGAGGCGGCGGCGGCGGGGGCTCCCGCAAAGCCTCGAACCCTGCGGGC AGCGGACCAAACCACCACGCGCCGCAGCTGTCTAGCGACTCGGTGCTGCCTCTCTATTCTCTGGGCTCCG GAGAGCGAGCGCACAACACCGGTGGCACCAAAGTCTTTCCGGAACGCAGCGGGAGCGGCAGTGCCAGTGG CAGCGGGGGTGGGGGCGACTTGGGCTTCCTGCACCTTGACTGTGCCCCAAGTAACTCGGATTTCTTCCTC AATGGGGGATACAGCTACCGTGGGGTCATTTTCCCAACCCTACGCAACTCCTTCAAGTCTCGGGATCTGG AGCGCCTCTATCAACGCTATTTCCTGGGCCAGAGGCGCAAATCGGAGGTAGTGATGAACGTGCTGGATGT ACTAACCAAACTCACCCTTCTAGTCCTTCACTTGAGCCTAGCCTCGGCTCCCATGGACCCTCTCAAGGGC ATCCTGCTAGGCTTTTTCACTGGCATCGAGGTGGTGATCTGCGCCCTCGTGGTGGTCAGGAAGGACACCA CCTCCCACACTTACCTGCAATACAGCGGCGTGGTCACTTGGGTGGCTATGACCACCCAGATTCTGGCAGC AGGCCTGGGCTATGGGCTTCTGGGCGACGGCATAGGCTACGTGCTTTTTACACTCTTCGCCACCTACAGC ATGCTTCCGCTGCCTCTCACCTGGGCCATCTTGGCCGGCCTGGGCACATCCTTGCTGCAAGTCACACTTC AAGTGCTCATACCCAGACTAGCGGTCTTTTCCATCAACCAGGTCCTGGCCCAGGTGGTGCTCTTCATGTG CATGAATACAGCAGGCATCTTCATCAGTTACCTTTCAGACCGCGCCCAGCGGCAGGCCTTCCTGGAGACC CGGAGGTGTGTGGAGGCCAGGCTCCGCCTGGAGACAGAGAACCAAAGACAGGAGCGGCTTGTGCTCTCTG TGCTCCCCAGGTTTGTCGTCCTAGAAATGATCAATGACATGACCAATGTGGAGGACGAGCACCTGCAGCA TCAGTTCCACCGCATCTACATCCATCGCTACGAGAACGTCAGTATTCTTTTTGCAGATGTCAAAGGATTT ACCAACCTCTCTACGACCTTGTCTGCTCAGGAGCTTGTCAGGATGCTCAACGAGCTCTTTGCCAGATTTG ATCGGCTGGCCCATGAGCATCACTGTCTTCGCATTAAAATCCTGGGGGACTGCTACTACTGTGTGTCAGG ACTGCCTGAGCCCCGCCAGGACCATGCTCATTGCTGTGTTGAAATGGGCCTCAGCATGATCAAAACTATC AGGTTTGTGAGGTCCAGAACGAAGCATGATGTTGACATGCGAATTGGAATCCATTCAGGCTCTGTGCTGT GTGGTGTGTTGGGCCTGAGAAAATGGCAGTTTGATGTCTGGTCTTGGGATGTGGACATCGCAAACAAACT TGAATCTGGAGGAATCCCTGGGAGAATTCACATTTCCAAAGCCACACTGGATTGCCTCAGTGGTGACTAT AATGTGGAAGAGGGCCACGGTAAGGAGAGGAATGAATTCTTGAGGAAGCATAATATAGAGACCTATTTGA TTAAGCAGCCCGAGGAGAGTTTGCTATCCTTGCCTGAAGATATAGTTAAGGAGTCGGTGAGCTGCTCGGA CAGGAGAAACAGTGGGGCAACGTTCACAGAAGGATCCTGGAGCCCAGAACTGCCATTCGACAACATCGTG GGCAAACAGAATACTCTGGCTGCCCTAACAAGAAATTCAATAAATCTGCTTCCAAACCATCTCGCACAAG CTTTGCATGTCCAGTCTGGGCCTGAGGAAATTAACAAGAGAATAGAGCATACCATCGACTTGCGGAGTGG CGATAAGTTGAGAAGAGAGCATATCAAGCCATTCTCACTGATGTTTAAAGACTCCAGCCTGGAGCACAAG TATTCTCAAATGCGGGATGAAGTATTCAAGTCAAACTTGGTCTGTGCATTTATCGTTCTTCTGTTTATCA CTGCGATTCAAAGTTTGCTTCCTTCTTCGAGGCTGATGCCTATGACCATCCAGTTCTCCATCCTGATCAT GCTGCACTCCGCCCTGGTCCTCATCACCACGGCAGAAGACTATAAGTGTCTGCCTCTCATTCTCCGCAAA ACCTGTTGTTGGATTAACGAGACCTATTTGGCCCGCAACGTCATCATCTTTGCTTCCATCTTGATTAATT TCCTGGGAGCCGTCATAAATATTCTGTGGTGTGATTTTGACAAGTCGATACCCTTGAAGAACCTGACTTT CAATTCCTCAGCTGTGTTTACAGATATCTGCTCCTACCCAGAGTACTTTGTCTTCACTGGGGTGTTGGCC ATGGTGACGTGTGCAGTATTTCTCCGGCTTAACTCTGTCCTGAAGCTGGCTGTGCTGCTCATTATGATCG CCATCTACGCCCTGCTGACAGAGACCATCTATGCAGGTCTCTTTCTGAGTTATGACAACCTGAACCACAG TGGAGAAGATTTTCTGGGGACCAAGGAGGCATCACTGCTACTGATGGCCATGTTCCTTCTTGCTGTATTC TACCATGGACAGCAGCTGGAGTACACAGCCCGCCTAGATTTCCTGTGGCGAGTACAGGCCAAAGAGGAGA TCAACGAGATGAAGGACTTGAGGGAACACAATGAGAACATGCTTCGCAATATCTTACCCGGCCACGTGGC CCGCCACTTCCTGGAGAAAGACAGAGACAATGAGGAGCTGTATTCTCAATCCTATGATGCCGTTGGGGTA ATGTTTGCCTCCATTCCTGGATTTGCAGACTTTTACTCTCAGACAGAAATGAACAACCAGGGAGTGGAAT GTCTGCGCTTGCTGAATGAGATCATTGCTGACTTTGATGAGTTACTTGGAGAGGACCGCTTTCAGGACAT AGAGAAGATTAAGACCATTGGTAGTACATACATGGCTGTCTCAGGACTGTCACCAGAGAAACAGCAATGT GAAGATAAATGGGGACATTTGTGTGCCCTGGCTGACTTCTCTCTTGCCCTGACTGAAAGCATACAAGAGA TCAACAAGCATTCGTTCAACAATTTTGAACTCCGTATTGGCATCAGCCATGGCTCAGTGGTAGCAGGTGT AATTGGCGCTAAGAAACCACAGTATGACATTTGGGGTAAAACTGTGAACTTGGCAAGCCGAATGGACAGC ACAGGAGTGAGTGGCCGGATCCAAGTTCCTGAGGAGACCTATCTCATCCTGAAGGATCAGGGCTTTGCCT TCGACTACCGGGGAGAGATATATGTGAAGGGCATCAGCGAACAAGAAGGGAAAATCAAAACATATTTTCT CCTGGGACGAGTCCAACCCAACCCATTCATCTTACCCCCAAGAAGACTTCCTGGGCAGTACTCTCTGGCT GCAGTTGTCCTTGGGCTTGTCCAGTCTCTCAACAGGCAAAGGCAGAAGCAACTACTCAATGAGAACAGCA ACTCTGGCATCATCAAGAGCCATTACAACCGGCGGACTTTGCTAACGCCAAGTGGGCCAGAGCCTGGAGC ACAGGCTGAAGGCACTGACAAATCCGATTTGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210573 representing NM_017142 Red=Cloning site Green=Tags(s) MELSDVHCLSGSEELYTIHPTPPAADGGSGSRPQRLLWQTAVRHITEQRFIHGHRGGGGGGSRKASNPAG SGPNHHAPQLSSDSVLPLYSLGSGERAHNTGGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFFL NGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLKG ILLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYS MLPLPLTWAILAGLGTSLLQVTLQVLIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLET RRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGF TNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTI RFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDY NVEEGHGKERNEFLRKHNIETYLIKQPEESLLSLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNIV GKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHK YSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRK TCCWINETYLARNVIIFASILINFLGAVINILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVLA MVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEASLLLMAMFLLAVF YHGQQLEYTARLDFLWRVQAKEEINEMKDLREHNENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGV MFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQQC EDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDS TGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLA AVVLGLVQSLNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEPGAQAEGTDKSDLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017142
ORF Size	3744 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017142.1, NP_058838.1
RefSeq Size	4601 bp
RefSeq ORF	3747 bp
Locus ID	29241
UniProt ID	P40146
MW	139.8 kDa
Gene Summary	may play a role in calcium/calmodulin regulated intracellular signaling; may mediate long term potentiation [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.