Agl (NM_001108564) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209001 representing NM_001108564 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACACAGTAAGCAGATTCGAATTTTACTATTGAACGAAATGGAGAAGCTAGAGAAGACCCTCTTCA GACTTGAGCAAGGATTTGAACTACAGTTCCGATTAGGCCCAACTTTACAAGGAAAAGCTGTTACTGTTTA TACAAATTACCCATTTCCTGGAGAAGCATTTAATCGAGAAAAATTCCGTTCCTTGGATTGGGAAAACCCA ACAGAGAGAGAAGATGATTCGGACAAATACTGTAAAGTTCATCTGCAGCAGTCTGGGTCTTTTCAGTACT ACTTCCTTCAAGGACAGGAGAAAAGTGGCGGAGGTTACATAGTTGTGGATCCTATTCTGCGAGTTGGAGC TGATAATCACGTGCTACCCTTGGATTGTGTTACTCTGCAGACATTTTTAGCAAAGTGTTTAGGACCCTTT GATGAGTGGGAAAGCCGACTACGGGTGGCAAAGGAGTCAGGCTACAACATGATTCACTTCACCCCGCTGC AGGCTCTCGGACTGTCCAGGTCATGCTATTCCCTTGCCGATCAGTTAGAATTAAATCCTGACTTCTCAAG GCCCAATAGAAGATACACCTGGGATGATGTTGGGCAGCTAGTAGAGAAGCTGAAGAGGGAGTGGAACATT CTTTGTATTACCGATGTGGTCTACAATCATACTGCTGCTAATAGTAAGTGGATCCATGAGCATCCAGAAT CTGCGTACAATCTTGTAAATTCTCCACACTTGAAACCTGCCTGGGTCCTAGACAGAGCGCTCTGGCATTT GTCCTGTGATGTTGCAGATGGGAAGTACAGAGAAAAAGGAGTCCCTGCTCTAATTGAAAATGACCATCAC ATGAACTGCATTCGGAAAATAATTTGGGAGGACATTTTCCCAAGGATTCAGCTCTGGGAGTTTTTCCAAG TAGATGTTCAGAAAGCGGTTGAACAATTTAGAAGACTGCTCTCTCAAGAAAACAGAAGAGTAACTAAGTC TGAACCGAAAGAACATCTTAAAATTATTCAGGACCCCGAATTCAGGCGGCTGGGCTGTGCTGTAGATATG AACATTGCTCTGGCGACCTTTATACCACATGACAATGGGCCAGCTGCAATTGAAGAGTGCTGTAATTGGT TCCGTAAGAGACTCGAGGAGTTAAATTCAGAGAAACACCACCTCACCAGCTGTCATCAGGAACAGGCCGT TAATTGTCTTTTGGGAAACGTGTTTTATGAACGGCTGGCGGGCCATGGCCCTAAACTAGGACCCGTCACC AGGAAGTACCCTTTAGTTACCAGGTATTTTACTTTCCCCTTTGAAGAAATGGCCTTGTCTGCAGAAGAGT CCCTGCTCCACCGCCCAGATAAAGCTTGCTTTCTGATGGCACACAATGGCTGGGTGATGGGCGATGACCC GCTTCGGAACTTTGCTGAGCCAGGTTCCAACGTTTATTTAAGGAGAGAACTTATTTGCTGGGGTGACAGT GTGAAATTGCGCTATGGGAACAAACCGGAGGACTGCCCTTACCTGTGGGCACACATGAAGAAATACACTG AGATCACTGCCACTCACTTCCAGGGAGTGCGCCTTGACAACTGCCACTCCACGCCTCTCCACGTGGCTGA GTACATGCTGGATGCTGCTAGGAAATTACAGCCCAATCTTTATGTAGTGGCTGAGCTGTTCACAGGAAGC GAAGATCTGGACAATATCTTCGTTACTAGACTGGGCATCAGTTCCTTAATAAGAGAGGCAATGAGCGCAT ATAACAGTCACGAAGAGGGCAGATTGGTCTACCGGTACGGAGGGGAGCCCGTCGGATCCTTCGTTCAGCC CTGTTTGAGGCCTCTTATGCCAGCTATCGCGCATGCCCTGTTTATGGACATCACCCACGATAATGAGTGT CCAATTGTGCACAGATCAGCCTATGATGCGCTTCCAAGTACCACAGTTGTCTCCATGGCGTGTTGTGCTA GTGGAAGTACAAGAGGCTACGACGAGCTAGTGCCTCATCAGATTTCAGTGGTTGCTGAAGAACGGTTTTA CACTAAGTGGAATCCTGGGGCATCCCCATCGGATACAGGAGATGTTAGCATCCACAGTGGGATTATTGCA GCCAGATGTGCCATCAACAGACTCCATCAAGAGCTTGGAGCCAAGGGTTTCATTCAAGTGTATGTGGATC AAGTGGATGAAGACATAGTGGCAGTAACCAGGCACTCACCAAGCATCCATCAGTCTGTTGTGGCTGTATC GAGAACGGCTTTTAGGAATCCCAAGACTTCATTTTACAGCAAAGAAGTGCCTCAAATGTGCATCCCTGGC AAAATTGAAGAGGTAGTTCTGGAAGCTAGAACTATTGAGAGGAATACAAAACCTTATAGGAAAGATGAGA ATTCAATCAATGGAATGCCGAACATGACAGTGGAACTTAGAGAACGTATCCAGCTTCATGAAAGTAAAAT TGTCAAACAAGCTGGAGTTGCCACAAAGGGTCCCAATGAATATATTCAGGAAATAGAATTTGAAAACCTA TCTCCAGGAAGTGTTATTATATTCAGAGTTAGCCTTGATCCACATGCGCAAGTTGCTGTTGGAATTCTTC GAAATCACCTGACCCAATTCAGCTCCCATTTTAAATCTGGAAGCCTTGCTGTTGACAACTCGGATCCTAT ATTAAAAATCCCCTTCGCTTCCATTGCCTCTAAATTGACTTTGGCTGAGCTAAATCAGGTACTTTACCGA TGTGAATCAGAAGAACAAGAAGACGGTGGAGGTTGCTATGATATACCAAACTGGTCGTCCCTTAAGTATG CAGGTCTGCAAGGATTAATGTCCGTGCTGGCAGAAATAAGACCAAAGAATGACTTGGGGCATCCCTTCTG TGAAAACTTGAGGTCTGGAGACTGGATGATCGACTACGTCAGTGGCCGGCTAATCTCACGATCAGGGAGT ATTGCTGAAGTTGGAAAATGGTTGCAGGCTATGTTCTTCTACCTGAAGCAGATCCCGCGATATCTCATCC CATGTTACTTTGATGCTATATTAATCGGTGCATATACCACTCTGCTGGATGTAGCCTGGAAACAGATGTC AAGCTTTGTTCAGAATGGCTCGACCTTCGTGAAGCACCTGTCCCTGGGTTCTGTCCAGATGTGTGGAGTC GGGAAGTGTCCTTGTCTGCCGCTGCTTTCACCCTCACTCCTGGATGTGCCGTGCAGGTTAAATGAGATCA CGAAGGAGAAAGAGCAGTGCTGTGCTTCCTTGGCTGCAGGCTTACCTCACTTTTCTTCTGGTCTTTTTCG CTGCTGGGGGAGGGATACTTTCATTGCACTTCGAGGGATGCTGCTGGTTACTGGTCGCTACCTAGAAGCC AGGAATATTATTTTAGCATTTGCTTGTACCCTGAGGCATGGTCTCATTCCTAACCTCCTTGGCGAAGGAA CGTATGCCAGGTACAACTGTCGTGATGCCGTGTGGTGGTGGCTGCAGTGCATTCAGGACTATTGCAAGAT GGTTCCAAATGGTCTAGACATCCTCAAATGCCCAGTTTCCAGAATGTATCCAACAGATGACTCTGCACCT TTGCCTGCTGGGACTCTGGATCAGCCTTTGTTTGAAGTCATACAGGAAGCTATGCAGAGGCACATGCAAG GCATTCAGTTCCGAGAGCGGAATGCCGGTCCACAGATCGATCGAAACATGAAGGATGAGGGTTTCAACAT AACCGTGGGGGTTGATGAAGAAACAGGATTTGTTTATGGAGGAAATCGCTTCAACTGTGGCACATGGATG GATAAAATGGGAGAGAGTGACAGAGCTAGAAACAGAGGGATCCCAGCCACCCCAAGAGATGGGTCTGCTG TGGAAATCGTGGGCTTGTGTAAATCTGCTGTTCGTTGGTTGCTGGAATTGTCCAAGAAAAACATTTTTCC TTATCATGAAGTCAGAGTAAGAAGACATGGAAAGGTTGTAGCAGTCTCCTATGATGAGTGGAACAGAAAA ATACAAAACAGTTTTGAAAAGCTCTTTCATGTTTCCGAAGACCCTTCAGATCCTAACGAGAAGCATCCGA ATCTGGTTCACAAGCGGGGCATCTACAAGGACAGCTACGGTGCGTCAAGTCCTTGGTGTGACTATCAGCT CAGGCCCAACTTTACCATAGCAATGGTCGTGGCCCCTGAGCTCTTTACTGCTGAAAAGGCTTGGAAGGCT TTGGAAATTGCAGAAGGAAAATTGCTTGGTCCCCTTGGCATGAAGACTTTGGATCCAGATGACATGGTTT ACTGTGGAGTCTATGACAATGCCTTAGACAATGACAACTACAACCTTGCCAGAGGTTTCAATTACCACCA AGGACCTGAGTGGCTATGGCCGATTGGGTATTTTCTTCGTGCAAAGTTGTATTTTTCCAAAATGATGGGC CCAGAGACTGCTGCGAAGACTGTATTTTTGGTTAAAAATGTTCTTTCTAGACATTATGTTCATCTTGAGA GATCCCCATGGAAAGGACTTCCAGAGCTCACCAATGAGAATGGCCAGTACTGCCCTTTCAGCTGTGAGAC ACAGGCATGGTCGATGGCTGCTGTTCTCGAAACCCTCTATGATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209001 representing NM_001108564 Red=Cloning site Green=Tags(s) MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPFPGEAFNREKFRSLDWENP TEREDDSDKYCKVHLQQSGSFQYYFLQGQEKSGGGYIVVDPILRVGADNHVLPLDCVTLQTFLAKCLGPF DEWESRLRVAKESGYNMIHFTPLQALGLSRSCYSLADQLELNPDFSRPNRRYTWDDVGQLVEKLKREWNI LCITDVVYNHTAANSKWIHEHPESAYNLVNSPHLKPAWVLDRALWHLSCDVADGKYREKGVPALIENDHH MNCIRKIIWEDIFPRIQLWEFFQVDVQKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEFRRLGCAVDM NIALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVT RKYPLVTRYFTFPFEEMALSAEESLLHRPDKACFLMAHNGWVMGDDPLRNFAEPGSNVYLRRELICWGDS VKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGS EDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNEC PIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPSDTGDVSIHSGIIA ARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPG KIEEVVLEARTIERNTKPYRKDENSINGMPNMTVELRERIQLHESKIVKQAGVATKGPNEYIQEIEFENL SPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNSDPILKIPFASIASKLTLAELNQVLYR CESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGS IAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGV GKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEA RNIILAFACTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAP LPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITVGVDEETGFVYGGNRFNCGTWM DKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVRRHGKVVAVSYDEWNRK IQNSFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKA LEIAEGKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMG PETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAAVLETLYDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108564
ORF Size	4596 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108564.1, NP_001102034.1
RefSeq Size	7567 bp
RefSeq ORF	4599 bp
Locus ID	362029
MW	174.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.