Gli2 (NM_001107169) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208592 representing NM_001107169 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACACCAGGAGGGAAGGTACCATTATGACCCTCACCCTGTCCACGGTGTGCACGGGCCTCCCACGC TAAGTGGCAGTCCTGTCATCTCAGATATCTCCTTGATACGACTTTCTCCACACCCTGCTGGCCCTGGAGA ATCGCCCTTCAGCGCCCACCACCCCTACATGAACCCCCACATGGAGCACTACCTCCGGTCTGTGCACAGC AGCCCCACACTCTCAATGATCTCTGCCGCCAGGGGCCTCAGCCCTGCTGATGTGGCCCATGAACATCTGA AAGAGAGGGGACTATTTAGTCTCGCTGCCCCAGGCACCAACCCTTCAGACTATTACCACCAGATGACCCT CATGGCAAGCCACCCCACACCCTACGGGGACATTCTAATGCAAAGTGGCGGTGCTGCTAGCGCACCCCAT CTCCATGACTACCTCAACCCTGTGGATGCATCACGATTCTCCAGCCCACGTGTGACCCCGCGACTGAGCC GCAAGCGGGCTCTATCCATCTCCCCGCTCTCAGATGCCAGCCTTGACCTACAGCGCATGATCCGGACCTC TCCCAACTCACTGGTAGCCTACATCAACAACTCCAGGAGCAGCTCAGCAGCCAGCGGTTCTTACGGGCAT CTGTCAGCTGGTGCCCTCAGCCCAGCCTTCACCTTCCCCCACCCCATCAATCCCGTGGCGTACCAGCAGA TCCTGAGCCAGCAGCGGGGCCTGGGTTCAGCCTTTGGACATACACCACCCCTGATCCAGCCTTCACCCAC CTTCTTGGCTCAGCAGCCCATGACTCTCACCTCCATCACCGTGCCTACCCAGCTAGGCAGCAATAGCAGC AACTGTATAAGTGATGCCAACCAGAATAAGCAGAACAGCGAGTCGGCCGTGAGCAGCACCGTGAACCCCA TCACCATCCATAAGCGGAGCAAGGTCAAGACTGAGGCTGAGGGCCTACGGCCAGCCTCCCCGCTTGGACT GACACAGGAGCAACTGGCTGATCTCAAGGAAGATCTGGACAGGGATGACTGTAAGCAGGAGGCTGAAGTG GTCATCTACGAGACCAACTGCCACTGGGCAGACTGCACGAAGGAATATGACACCCAGGAGCAGCTGGTGC ATCACATCAACAATGAGCACATCCACGGGGAGAAGAAGGAGTTCGTGTGCCGCTGGCAGGCCTGCACGAG AGAGCAGAAGCCCTTCAAGGCCCAGTACATGCTGGTTGTCCACATGCGCAGGCACACGGGTGAGAAGCCG CACAAGTGCACGTTTGAAGGCTGCTCAAAGGCCTACTCTCGCCTGGAGAACTTGAAGACACACCTGCGGT CCCACACAGGGGAGAAGCCCTATGTGTGTGAACACGAAGGCTGCAACAAAGCCTTCTCCAATGCCTCGGA CCGTGCCAAGCACCAGAACCGCACTCACTCCAATGAGAAACCCTACATCTGTAAGATCCCAGGCTGCACT AAGAGGTACACAGACCCCAGCTCACTCCGCAAGCATGTGAAGACCGTCCACGGGCCAGATGCCCATGTGA CCAAGAAACAGCGTAATGATGTGCACCTCCGTGCTCCACTGCTCAAGGAGAATGGGGACAATGAGGCCGG TGCTGAGCCAGGTGGCCGGGGACCCGAGGAGAGTGTGGAGGCCAGTAGCACCAGTCACACTGCGGAGGAC TGCCTGCATATCAAAGCCATCAAGACAGAGAGCTCCGGGCTGTGCCAGTCCAGCCCCGGGGCCCAGTCAT CCTGCAGCAGCGAGCCCTCTCCCCTGGGCAGTGTCCCCAACAATGACAGTGGCGTGGAGATGCCGGGGAC AGGGCCCGGGAGTCTGGGAGACCTGACAGCACTGGACGACACGTCTCCAGGAGCTGACACATCAGCCCTG GCTGTACCTTCCACTGGTGGCCTGCAGCTGCGCAAACACATGACCACCATGCATCGCTTTGAGCAGCTGA AGAGAGAGAAGCTCAAGTCTCTAAAGGATTCCTGCTCGTGGGCTGGCCCAGCCCCACACACCCGCAACAC CAAGCTGCCTCCCCTTCCAGTGAATGGTTCTGTTCTGGAAAACTTCAACAATACTGGGGGCTGTGGACCA GCGGGACTGCTGCCCAACCAGCGGCTACCAGAACTGACAGAAGTGACCATGCTGAGCCAGCTGCAGGAAC GGAGAGACAGCTCCACCAGCACTATGAGCTCAGCCTACACCGTGAGCCGCCGCTCCTCCGGCATCTCCCC ATACTTCTCCAGCCGCCGTTCCAGCGAGGCCTCGCCTCTTGGTGGCATACGCCCGCACAATGCCAGCTCA GCGGACTCCTACGACCCCATCTCCACAGACGCCTCTCGGCGCTCCAGTGAGGCCAGTCAGTGCAGCGGTG GTGGCCCCGGGCTGCTCAACCTGACACCCGCACAGCAGTATAGCCTGCGTGCCAAGTACGCAGCGGCCAC AGGCGGGCCACCACCCACGCCACTGCCGGGCCTCGACCGCGTCAGCCTTCGTACCCGCCTGGCATTACTA GATGCTCCTGAGCGTGCACTGCCAGGCGCCTGCCCACAACCTCTGGGGCCACGGCGTGGTAGCGACGGGC CTACCTATAACCACGGCCATGGCTATGTAGGTGCGGCTCCTGCCTTACCCCATGAGGGGCCTAACGGCAG CACACGGCGGGCCAGCGACCCTGTGCGGCGACCTGACCCCCTTGTTCTGCCTCGAGTGCAACGTTTCCAC AGCATCCACAACATGAATCCAGGTCCGCTGCCCCCCTGCACTGATCGGCGTGGCCTGCATTTACAGAGCC ACCCCAGCGTAGATGGCAGCCTGACCCGCCATGCCTACTCTCCGCGACCCCCTAGCATCAATGAGAACGT GGTGATGGAAGCCGTGGCTGCTGGGGTAGAGGGTGCCGGGCTAGAGTCTGACCTGGGGCTGGTGGAGGAT GACCTCGTGCTGCCAGATGATGTGGTACAGTACATCAAGGCTCACACAGGTGGTGCCTTGGATGACAGCA CACGGCAGGTGTATCCCACAGAAGGTACTGGCTTCCCCGAGAACTCTAAACTGCCCAGTCCCGGGCTACA AGGCCACCGCAGGCTAGCGGCTGCCGACTCCAACATGAGCCCTTCTGCTCCTGGGCTGGGAGGCTGCCAG CTGAGCTACAGCCCCTCCTCCAGCCTCAACAAGAGCAACATGCCTGTGCAGTGGAACGAGGTGAGTTCTG GCACCGTGGATGCCCTGCCCACCCAGGTGAAGCCACCCTCTTTTCCTCAGAGCAACCTGGTTCAGCAGAA GCCAGCCTTTGCCCACTATCCAGGCTATAGCCCACAAGCCCTGCAGAGCGGCTCTGGAGGTCTAGACAGC ACCCAACCACACCTACAGCTTCGAGGGGCCCCCTCTGCATCAAGAGGGAGCTACACGCAACAGCCTCGAC TGCCAGCTACAGGCAGTCAGTGCCTGGGTGTAACTGCAGCCATGAGCCCTCAGGCCAACTACAGCCAAAC CCACCACCAGCTGAGTCCAAACGTCGTCAGTGGATCTCTGAACCAGTTTGCCCCCTCCTGCAGCAATATG GCAGCCAAGCCTAGCCACCTGGGACTCCCTCAGCAAATGGAAGTTGTCCCCAATGCCACCATCATAAGTG GCCATCAACGGGAACTTGGGATTGCCAGTTCATCCCTGGCTGCAGTGTCACAACCTCACCCAGTCCTGGG CTATCCCCAGCAGGACGGCTACCAACAGGTCTCTGGCCTTCCGTCATCCCATCAGCCTAGCTTCATGGAG TCCCAGCAGAACACGGGCTTTGGTCTCATGCAACCTCGGCCGCCCCTGGAGCCCAACACAGCTAGTCGTC ACCGAGGAGTACGTTCTGGCCAACAGCAGCAGCAGCTGTATGCCAGGACCGCTGGCCAAGCCGTGGTCAC ATCAGCCAACCAAGAGACAGCAGAAGCTATGCCCAAGGGAACAGCAGGGACCGTGGTATCCCTAACTCCT CAGCCATCTCAGGACACAGGGCGGTCACAGGACCAGAACACGCTCTACTACTATGGCCAGATCCACATGT ATGAACAGAACGGAGGCTGCCCAGCCATGCAGCCCCAGCCGCCACAACCACAAGGTTGCTCAGACAATAT CCAGCCTGAACCTCTGCCTTCACCAGGAGTCAACCAGGTGTCCAGCACCGTGGACTCCCAGCTCCTGGAG CCCCCCCAGATTGACTTTGATGCCATCATGGACGATGGCGATCACTCGAGTTTGTTCTCTGGTGCACTGA GCCCAAGCCTGCTCCACAATCTCTCCCAGAATTCCTCACGCCTCACCACACCCCGGAATTCCCTGACACT GCCCTCCATCCCCGTGGGCATCAGCAACATGGCTGTTGGGGACATGAGTTCCATGCTCACCAGCTTGGCT GAAGAGAACAAGTTTTTAAACATGATGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208592 representing NM_001107169 Red=Cloning site Green=Tags(s) MRHQEGRYHYDPHPVHGVHGPPTLSGSPVISDISLIRLSPHPAGPGESPFSAHHPYMNPHMEHYLRSVHS SPTLSMISAARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDILMQSGGAASAPH LHDYLNPVDASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGH LSAGALSPAFTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSITVPTQLGSNSS NCISDANQNKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEV VIYETNCHWADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKP HKCTFEGCSKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCT KRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVHLRAPLLKENGDNEAGAEPGGRGPEESVEASSTSHTAED CLHIKAIKTESSGLCQSSPGAQSSCSSEPSPLGSVPNNDSGVEMPGTGPGSLGDLTALDDTSPGADTSAL AVPSTGGLQLRKHMTTMHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGCGP AGLLPNQRLPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGIRPHNASS ADSYDPISTDASRRSSEASQCSGGGPGLLNLTPAQQYSLRAKYAAATGGPPPTPLPGLDRVSLRTRLALL DAPERALPGACPQPLGPRRGSDGPTYNHGHGYVGAAPALPHEGPNGSTRRASDPVRRPDPLVLPRVQRFH SIHNMNPGPLPPCTDRRGLHLQSHPSVDGSLTRHAYSPRPPSINENVVMEAVAAGVEGAGLESDLGLVED DLVLPDDVVQYIKAHTGGALDDSTRQVYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMSPSAPGLGGCQ LSYSPSSSLNKSNMPVQWNEVSSGTVDALPTQVKPPSFPQSNLVQQKPAFAHYPGYSPQALQSGSGGLDS TQPHLQLRGAPSASRGSYTQQPRLPATGSQCLGVTAAMSPQANYSQTHHQLSPNVVSGSLNQFAPSCSNM AAKPSHLGLPQQMEVVPNATIISGHQRELGIASSSLAAVSQPHPVLGYPQQDGYQQVSGLPSSHQPSFME SQQNTGFGLMQPRPPLEPNTASRHRGVRSGQQQQQLYARTAGQAVVTSANQETAEAMPKGTAGTVVSLTP QPSQDTGRSQDQNTLYYYGQIHMYEQNGGCPAMQPQPPQPQGCSDNIQPEPLPSPGVNQVSSTVDSQLLE PPQIDFDAIMDDGDHSSLFSGALSPSLLHNLSQNSSRLTTPRNSLTLPSIPVGISNMAVGDMSSMLTSLA EENKFLNMMT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107169
ORF Size	4440 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107169.1, NP_001100639.1
RefSeq Size	6146 bp
RefSeq ORF	4443 bp
Locus ID	304729
MW	158.6 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.