Apaf1 (NM_023979) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207514 representing NM_023979 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCAAAGGCCCGAAATTGTTTGCTTCAACATAAAGAAGCTCTGGAAAAGGACATCAAAACATCCT ACATCATGGATCACATGATCAGTAATGGCGTCTTAACAGTAGTAGAGGAGGAAAAGGTCAAAAGTCAGGC CACTCAATATCAACGAGCAGCTGCTTTAATTAAAATGATACTTAATAAAGACAATTATGCCTACATTTCA TTCTACAACGCTCTACTACACGAGGGCTATAAAGACCTTGCTGGACTTCTGCATAGCGGCCTTCCTCTTG TGTCTTCTTCCAGCGGCAAGGACACAGACGGTGGAAACACTTCATTTGTAAGGACAGTGCTGTGTGAAGG TGGAGTGCCACAGAGGCCAGTCATCTTTGTCACTAGGAAGAAGCTGGTCAGTGCGATTCAGCAGAAGCTC TGGAAACTGAATGGGGAGCCAGGGTGGGTCACCATCTATGGAATGGCAGGCTGTGGCAAGTCTGTATTAG CTGCGGAAGCTGTTCGCGACCATGCCCTCCTAGAAGGTTGTTTTTCAGGGGGCGTACACTGGGTTTCCAT CGGAAAACAAGACAAGTCCGGGCTTCTAATGAAGCTGCAGAATCTTTGCACGCGGTTGGGTCAAGAAGAG AGTTTCTCTCAGAGGCTCCCGCTTAACATTGAGGAGGCCAAAGACCGTCTCCGTGTTCTGATGCTGCGAA AACACCCAAGGTCTCTGTTGATCTTGGATGACGTTTGGGATCCTTGGGTGTTAAAGGCTTTTGACAACCA GTGTCAGATTCTTCTTACCACCAGAGATAAGAGTGTTACCGATTCAGTAATGGGTCCTAAGTATGTTATC CCTGTGGAGAGTGGTCTAGGGAAAGAGAAGGGACTTGAAATCTTGTCACTTTTTGTTAATATGAAGAAAG AAGATCTGCCAGTGGAGGCTCACAGTATTATAAAGGAGTGCAAAGGCTCTCCTCTCGTCGTGTCTTTAGT TGGTGCGCTTTTACGGGATTTTCCCAATCGCTGGGCATACTACCTCAGGCAGCTTCAGAATAAGCAGTTT AAGAGAATAAGGAAATCTTCATCTTATGACTACGAGGCTCTGGATGAAGCCATGTCCATAAGTGTGGAGA TGCTCAGAGAAGACATCAAGGACTACTACACAGACCTCTCCATCCTTCAGAAGGATGTCAAGGTGCCTAC GAAGGTGCTGTGTGTTCTCTGGGACTTGGAAACGGAAGAAGTTGAAGACATCTTGCAGGAGTTCGTTAAT AAGTCTCTCTTATTCTGTAATCGGAATGGGAAGTCATTTTGTTATTATTTACACGATCTTCAAGTAGATT TCCTTACAGAGAAGAATCGAAGTCAGCTCCAGGATCTGCACAGGAAGATGGTCACTCAATTTCAGAGGTA TCACCAGCCGCATACGCTGTCTCCAGGTCAGGAGGACTGCATGTACTGGTACAACTTCCTGGCCTATCAT ATGGCCAGTGCCGGTATGCACAAAGAACTTTGTGCTTTAATGTTTTCCCTGGATTGGATTAAAGCAAAAA CAGAACTTGTCGGCCCTGCGCATCTGATTCATGAGTTCGTGGAATATAGGCATATACTGGATGAAAAGGA TTGTGCAGTCTGTGAGAATTTTCAGGAGTTTTTATCTTTAAACGGACACCTTCTTGGACGACAGCCATTT CCTAACATTGTGCAACTGGGCCTCTGTGAACCAGAGACTTCCGAAGTTTATCAACAAGCAAAGCTGCAGG CCAAGCAGGAGGTGGATACCGGGCGGCTTTACCTGGAATGGATCAACAAAAAAACCATCAAGAATCTGTC CCGCTTAGTCGTTCGCCCCCATACAGACGCCGTGTACCATGCGTGTTTTTCTCAGGATGGTCAGAGAATA GCTTCTTGTGGAGCTGATAAAACCTTGCAGGTGTTTAAAGCCGAGACAGGAGAGAAACTTCTTGACATTA AAGCTCATGAAGATGAGGTGCTTTGCTGTGCGTTCTCCTCAGATGACAGTTACATAGCAACCTGCTCAGT GGATAAGAAAGTTAAGATTTGGGATTCTGGGACTGGGAAGCTAGTGCACACCTACGAAGAACACTCAGAG CAGGTCAATTGCTGCCATTTCACCAATAAGAGCAACCACCTTCTTTTGGCCACCGGGTCAAATGACTCCT TCCTTAAGCTCTGGGATTTGAATCAAAAAGAATGCCGAAATACCATGTTCGGTCACACGAACTCAGTCAC CCACTGCAGGTTCTCACCAGACGATGAGCTCTTGGCGAGTTGCTCAGCCGACGGGACTTTAAAGCTTTGG GATGTGAGATCAGCAAACGAGAAGAAAAGCATTAATGTGAAGCGCTTCTTCCTGAGTTCAGAAGACCCAC CAGAGGACGTGGAGGTGATCGTGAAATGTTGCTCCTGGTCTGCAGATGGCGACAGAATAATAGTGGCAGC AAAAAACAAAGTCCTCCTTCTTGATATTCATACTAGTGGCCTGCTGACTGAGATCCACACAGGTCATCAC AGCACCATCCAGTACTGTGACTTCTCCCCCTACGACCATTTGGCAGTGATTGCCCTGTCTCAGTACTGTG TGGAGTTGTGGAACATAGACTCCCGGGTAAAGGTGGCTGACTGCAGGGGACATTTAAGTTGGGTCCACGG TGTGATGTTTTCTCCTGATGGCTCCTCGTTCTTGACAGCTTCTGATGACCAAACAATAAGGGTCTGGGAG ACAAGAAAGGTGTGCAAGAACTCTGCCATTGTGTTAAAGCAGGAAATAGATGTCGTGTTTCAAGAAAACG AAATGATGGTCCTTGCAGTTGACAACATAAGAGGCCTACAACTCATTGCTGGAAAAACAGGCCAGATTGA TTACCTGCCTGAAGCCCAAGTGAGTTGCTGCTGCCTAAGTCCACATCTTGAGTACGTTGCATTTGGAGAT GAAGAGGGAGCCATTAAGATTATAGAACTTCCAAACAACAGAGTTTTCAGCTCTGGGATTGGGCACAAGA AAGCTGTACGGCACATCCAGTTCACAGCTGATGGGAAGACACTGATTTCAAGTTCTGAAGACTCTGTGAT TCAGGTGTGGAATTGGCAGACAGAGGAGTATGTATTTCTGCAAGCCCATCAGGAAACAGTAAAGGACTTC AGACTCCTGAGAGATTCACGATTGCTTTCCTGGTCATTCGATGGAACGGTGAAGGTGTGGAATGTCATTA CCGGAAGAATTGAAAGAGACTTTACTTGTCATCAGGGCACAGTGCTTTCCTGTGCTATCTCTTCTGATGC CACCAAGTTTTCATCTACCTCAGCTGATAAGACCGCCAAGATCTGGAGTTTTGAACTCCCTTCCCCTCTT CATGAGCTGAAGGGCCATAACAGCTGTGTCCGCTGCTCTGCCTTCTCGCTGGATGGCATCCTGTTAGCAA CTGGAGATGACAATGGAGAAATCAGGATATGGAATGTCTCAGATGGCCAGCTTCTTCATTTGTGTGCTCC GATTTCCATAGAAGAAGGAACGGCTACCCACGGAGGCTGGGTGACTGATGTTTGCTTCTCTCCTGACAGA AAAATGCTTGTCTCTGCCGGAGGATACCTTAAGTGGTGGAATGTTGTTACTGGGGAGTCCTCACAGACCT TCTACACCAATGGAACAAACCTCAAGAAAATACATGTGTCCCCTGACTTCAGAACATATGTGACCGTTGA TAATCTTGGTATTTTATATATTTTACAGGTTTTAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207514 representing NM_023979 Red=Cloning site Green=Tags(s) MDAKARNCLLQHKEALEKDIKTSYIMDHMISNGVLTVVEEEKVKSQATQYQRAAALIKMILNKDNYAYIS FYNALLHEGYKDLAGLLHSGLPLVSSSSGKDTDGGNTSFVRTVLCEGGVPQRPVIFVTRKKLVSAIQQKL WKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHALLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCTRLGQEE SFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKYVI PVESGLGKEKGLEILSLFVNMKKEDLPVEAHSIIKECKGSPLVVSLVGALLRDFPNRWAYYLRQLQNKQF KRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVN KSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYHQPHTLSPGQEDCMYWYNFLAYH MASAGMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVCENFQEFLSLNGHLLGRQPF PNIVQLGLCEPETSEVYQQAKLQAKQEVDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRI ASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSVDKKVKIWDSGTGKLVHTYEEHSE QVNCCHFTNKSNHLLLATGSNDSFLKLWDLNQKECRNTMFGHTNSVTHCRFSPDDELLASCSADGTLKLW DVRSANEKKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDRIIVAAKNKVLLLDIHTSGLLTEIHTGHH STIQYCDFSPYDHLAVIALSQYCVELWNIDSRVKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWE TRKVCKNSAIVLKQEIDVVFQENEMMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGD EEGAIKIIELPNNRVFSSGIGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTEEYVFLQAHQETVKDF RLLRDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFELPSPL HELKGHNSCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHLCAPISIEEGTATHGGWVTDVCFSPDR KMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_023979
ORF Size	3747 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_023979.1, NP_076469.1
RefSeq Size	6111 bp
RefSeq ORF	3750 bp
Locus ID	78963
UniProt ID	Q9EPV5
MW	141.2 kDa
Gene Summary	protein involved in the initiation of apoptosis [RGD, Feb 2006]

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