Myh10 (NM_031520) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MCH-B; NMMHC-B; SMemb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207155 representing NM_031520 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACC CTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCTGAACGCCATGGTTTTGAGGC AGCTAGTATTAAAGAAGAACGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAACGGGAAGAAAGCGATG GTCAACAAAGATGACATTCAGAAGATGAACCCGCCGAAGTTCTCCAAGGTGGAGGACATGGCAGAGCTCA CGTGCTTGAACGAAGCCTCTGTTTTACACAATCTGAAGGATCGCTACTATTCAGGACTCATCTATACTTA CTCGGGACTCTTCTGTGTGGTGATAAATCCCTACAAGAACCTTCCGATTTACTCTGAGAATATTATTGAG ATGTACAGAGGGAAGAAGCGCCATGAGAAGCCGCCACACATCTACGCCATATCAGAGTCTGCCTACAGAT GCATGCTTCAAGATCGTAAGGACCAGTCGATTCTTTGCACGGGTGAATCAGGTGCCGGGAAGACAGAAAA TACAAAGAAAGTCATTCAGTACCTTGCCCACGTTGCTTCTTCACACAAAGGAAGAAAGGACCACAATATT CCTGGGGAACTTGAACGGCAGCTTTTACAAGCAAATCCAATTCTGGAATCATTTGGAAATGCGAAGACTG TGAAAAATGATAACTCATCTCGCTTTGGCAAGTTTATCCGGATCAACTTTGATGTAACCGGCTATATTGT TGGGGCCAACATTGAAACATACCTTCTGGAAAAGTCCCGTGCTGTTCGTCAAGCTAAAGATGAACGCACG TTTCATATCTTTTATCAGTTGCTCTCTGGAGCAGGGGAACACCTGAAGTCCGACTTACTCCTGGAAGGCT TTAATAACTACAGATTCCTCTCCAACGGCTACATCCCTATTCCTGGACAGCAAGACAAGGATAACTTTCA GGAGACCATGGAAGCCATGCACATAATGGGCTTCTCTCACGAAGAGATCCTCTCAATGCTTAAAGTCGTG TCTTCAGTGCTGCAGTTTGGAAACATCTCTTTCAAAAAGGAGAGGAACACTGACCAAGCTTCCATGCCCG AGAACACAGTTGCACAGAAACTCTGCCACCTGCTTGGGATGAATGTGATGGAGTTCACTCGGGCTATCCT CACGCCCAGGATCAAAGTTGGCCGAGATTATGTACAGAAAGCCCAGACCAAAGAGCAGGCAGATTTTGCA GTGGAAGCATTGGCAAAGGCTACCTATGAGCGGTTGTTTCGCTGGCTCGTTCACCGCATCAATAAGGCGC TGGATAGGACCAAACGCCAGGGAACGTCTTTCATTGGGATCCTGGATATTGCTGGTTTTGAAATTTTTGA GCTGAACTCCTTTGAACAACTATGCATCAACTACACCAATGAGAAGCTGCAGCAGCTGTTCAACCACACC ATGTTCATCCTGGAGCAGGAGGAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTTGGTCTCG ACTTGCAGCCCTGCATCGACCTGATAGAGAGACCTGCCAATCCCCCTGGTGTGCTGGCCCTCCTGGATGA AGAATGCTGGTTCCCCAAAGCCACAGACAAAACGTTTGTTGAGAAGCTGGTTCAGGAGCAAGGTTCCCAC TCCAAGTTTCAGAAACCACGGCAACTGAAAGACAAAGCCGACTTCTGCATCATCCACTATGCAGGGAAGG TGGACTATAAGGCGGATGAGTGGCTGATGAAGAACATGGACCCCCTGAATGACAACGTGGCCACCCTCCT GCACCAGTCATCAGACAGATTTGTCGCCGAGCTTTGGAAGGATGTGGACCGAATTGTAGGTCTGGATCAA GTCACTGGCATGACTGAGACAGCTTTTGGCTCTGCATATAAAACCAAGAAGGGCATGTTCCGCAACGTCG GGCAGCTCTACAAAGAGTCTCTCACCAAGCTCATGGCAACCCTCCGAAACACCAACCCCAACTTCGTACG CTGCATCATTCCAAATCACGAGAAGCGGGCTGGAAAACTGGATCCACATCTTGTGCTGGATCAGCTCCGC TGTAACGGTGTCCTGGAAGGAATCAGGATCTGTCGCCAGGGATTCCCCAACCGGATAGTTTTCCAGGAGT TCAGACAGAGATATGAGATCCTAACTCCCAACGCTATTCCCAAAGGCTTCATGGATGGCAAACAGGCCTG TGAGCGAATGATCCGAGCTTTAGAACTGGACCCAAACCTGTATAGAATTGGACAGAGCAAGATATTTTTC CGAGCTGGAGTTCTGGCGCACTTAGAGGAAGAAAGAGATTTAAAAATCACTGATATAATCATCTTTTTCC AAGCTGTATGCAGAGGCTACCTAGCCAGAAAGGCCTTTGCCAAGAAACAGCAGCAGCTAAGTGCCTTAAA GGTCTTGCAGCGGAACTGTGCGGCGTACCTGAAGCTGCGACACTGGCAGTGGTGGCGCGTCTTCACAAAG GTGAAGCCTCTCCTCCAAGTGACCCGCCAGGAGGAGGAGCTCCAGGCTAAAGATGAGGAGCTGCTGAAGG TGAAAGAGAAGCAGACAAAAGTGGAAGGGGAACTTGAGGAGATGGAACGGAAGCACCAGCAGCTGCTGGA AGAGAAGAATATCCTGGCAGAACAGCTGCAGGCCGAGACTGAGCTCTTTGCTGAAGCAGAGGAGATGAGA GCAAGACTCGCTGCCAAGAAGCAGGAACTGGAGGAGATCCTCCATGACCTGGAGTCCAGGGTGGAGGGAG AGGAAGAGCGAAACCAGATCCTGCAGAACGAGAAGAAGAAGATGCAAGCGCACATTCAGGATCTAGAAGA GCAACTGGATGAGGAGGAGGGGGCGCGGCAAAAGCTGCAGCTGGAGAAGGTGACCGCGGAGGCTAAAATC AAGAAGATGGAAGAGGAGGTTCTGCTTCTCGAGGACCAGAATTCCAAATTTATCAAAGAAAAGAAACTCA TGGAAGATCGAATTGCTGAGTGTTCCTCTCAGCTGGCTGAAGAGGAAGAAAAGGCAAAAAACTTAGCCAA AATCAGGAATAAGCAAGAAGTGATGATCTCGGATTTAGAAGAACGCTTGAAGAAGGAGGAGAAAACACGG CAGGAACTGGAAAAGGCCAAACGGAAACTGGACGGTGAAACAACTGATCTGCAGGACCAGATCGCCGAGT TGCAAGCACAGGTCGATGAGCTCAAAGTCCAGTTGACCAAGAAGGAGGAGGAGCTTCAGGGGGCGCTGGC CAGAGGAGATGATGAGACGCTGCACAAGAATAATGCGCTTAAAGTTGCGCGGGAGCTGCAGGCCCAAATC GCCGAGCTCCAGGAAGACTTTGAGTCTGAAAAGGCTTCAAGGAACAAGGCTGAGAAACAAAAACGGGACC TGAGTGAGGAGCTGGAAGCTCTAAAGACAGAACTGGAGGACACCCTAGACACCACAGCAGCTCAGCAGGA ACTCCGCACAAAGCGGGAGCAGGAAGTAGCTGAGCTGAAGAAGGCTCTTGAGGATGAAACTAAGAACCAC GAAGCTCAGATCCAGGACATGAGACAGAGGCACGCCACGGCACTGGAGGAACTCTCTGAGCAACTGGAAC AGGCTAAGAGGTTCAAAGCCAATCTGGAGAAGAACAAACAGGGCCTGGAGACGGACAACAAGGAGCTGGC GTGTGAGGTGAAGGTGCTGCAGCAAGTCAAGGCTGAGTCAGAGCACAAGAGGAAGAAGCTCGATGCCCAG GTCCAGGAGCTCCACGCCAAGGTGTCAGAAGGTGACAGGCTCAGGGTAGAACTGGCCGAGAAAGCAAACA AGCTACAGAATGAGCTGGATAATGTCTCAACCCTGCTGGAAGAAGCCGAGAAGAAAGGAATGAAGTTCGC TAAGGACGCCGCAGGTCTCGAGTCTCAGCTACAGGACACACAGGAGCTCCTTCAGGAGGAGACGCGGCAG AAACTGAACCTGAGCAGTCGGATCCGGCAGCTGGAGGAGGAGAAGAACAGCCTTCAGGAGCAGCAGGAGG AGGAAGAGGAGGCCAGGAAGAACTTGGAAAAGCAGGTGTTGGCCCTGCAGTCCCAGCTGGCTGACACCAA GAAGAAAGTGGACGATGACCTGGGGACAATTGAGGGTTTGGAAGAAGCCAAGAAGAAACTGCTTAAGGAC GTGGAGGCACTGAGTCAGCGACTGGAGGAAAAGGTCCTAGCATATGACAAGCTGGAGAAGACCAAGAACC GGCTGCAACAGGAGCTGGATGACCTGACGGTGGACCTGGACCACCAGCGCCAGATCGTCTCCAACTTGGA GAAGAAACAGAAGAAGTTTGACCAGCTGTTGGCAGAAGAGAAGGGCATCTCTGCTCGCTATGCAGAAGAA CGGGACCGGGCTGAAGCTGAGGCCAGAGAGAAAGAAACCAAGGCGCTCTCCCTGGCGCGGGCCCTGGAGG AGGCCTTGGAGGCCAAGGAGGAATTCGAGAGGCAGAATAAGCAGCTTCGGGCAGACATGGAAGACCTCAT GAGCTCGAAAGATGATGTGGGGAAGAACGTCCACGAGCTGGAGAAGTCCAAGCGGGCCCTGGAGCAGCAG GTGGAGGAGATGAGGACCCAGCTGGAGGAGCTGGAGGACGAGTTGCAGGCCACTGAGGACGCCAAACTCC GCCTGGAAGTCAACATGCAGGCCATGAAGGCCCAGTTTGAGAGGGACCTGCAAACCAGAGATGAGCAGAA TGAAGAGAAGAAGCGGCTGCTGCTCAAGCAGGTACGGGAGCTCGAGGCAGAGCTGGAGGATGAGCGGAAG CAGCGGGCACTTGCTGTGGCTTCAAAGAAGAAGATGGAGATAGACCTGAAGGACCTGGAGGCTCAGATCG AGGCCGCCAACAAGGCCCGGGATGAAGTGATCAAGCAGCTTCGCAAGCTTCAGGCACAGATGAAGGATTA CCAGCGTGAACTAGAAGAAGCTCGAGCATCTAGAGATGAGATTTTTGCTCAATCCAAAGAAAGTGAAAAG AAACTGAAGAGTCTAGAAGCAGAAATCCTCCAGTTGCAAGAGGAGCTGGCCTCATCTGAGCGAGCCCGCA GACACGCAGAGCAGGAGCGAGATGAGCTGGCTGACGAGATTGCCAACAGCGCCTCTGGGAAGTCCGCTCT GCTGGATGAGAAGCGGCGCCTGGAAGCCCGGATCGCGCAGCTGGAGGAGGAGCTAGAAGAGGAGCAGAGT AACATGGAGCTGCTCAATGACCGCTTCCGCAAGACCACGCTCCAGGTGGACACCCTGAACACAGAGTTGG CAGCGGAGCGCAGCGCTGCCCAGAAGAGCGACAATGCCCGCCAGCAGCTGGAGCGACAAAACAAGGAGCT GAAGGCCAAGCTGCAGGAGCTGGAGGGGGCGGTCAAGTCCAAGTTCAAAGCCACCATCTCAGCCCTGGAA GCCAAGATTGGGCAGCTGGAGGAACAGCTGGAGCAGGAAGCCAAGGAACGCGCAGCTGCCAACAAACTAG TCCGTCGGACAGAGAAGAAACTGAAAGAGATCTTCATGCAGGTCGAAGACGAGCGCCGGCATGCGGATCA GTATAAGGAGCAGATGGAGAAAGCCAATGCCAGGATGAAGCAGCTTAAACGCCAGCTGGAAGAAGCTGAG GAGGAGGCTACACGTGCCAATGCATCTCGGCGTAAACTCCAAAGGGAACTGGATGATGCCACCGAGGCCA ATGAGGGCCTGAGCCGTGAGGTCAGCACTCTCAAGAACCGGCTTAGGCGGGGCGGCCCAATCAGCTTTTC TTCAAGCCGATCTGGCCGGCGCCAGCTACACATTGAGGGGGCATCGCTAGAGCTGTCAGATGACGACACA GAAAGCAAGACCAGTGATGTCAATGAGACACAGCCACCCCAGTCGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207155 representing NM_031520 Red=Cloning site Green=Tags(s) MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAM VNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIE MYRGKKRHEKPPHIYAISESAYRCMLQDRKDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNI PGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERT FHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVV SSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFA VEALAKATYERLFRWLVHRINKALDRTKRQGTSFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHT MFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSH SKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQ VTGMTETAFGSAYKTKKGMFRNVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLR CNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFF RAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTK VKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMR ARLAAKKQELEEILHDLESRVEGEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKI KKMEEEVLLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTR QELEKAKRKLDGETTDLQDQIAELQAQVDELKVQLTKKEEELQGALARGDDETLHKNNALKVARELQAQI AELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEDETKNH EAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQ VQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGMKFAKDAAGLESQLQDTQELLQEETRQ KLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLALQSQLADTKKKVDDDLGTIEGLEEAKKKLLKD VEALSQRLEEKVLAYDKLEKTKNRLQQELDDLTVDLDHQRQIVSNLEKKQKKFDQLLAEEKGISARYAEE RDRAEAEAREKETKALSLARALEEALEAKEEFERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQ VEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLLKQVRELEAELEDERK QRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEK KLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQS NMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALE AKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAE EEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDT ESKTSDVNETQPPQSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031520
ORF Size	5928 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031520.1, NP_113708.1
RefSeq Size	7648 bp
RefSeq ORF	5931 bp
Locus ID	79433
UniProt ID	Q9JLT0
MW	229 kDa
Gene Summary	nonmuscle myosin II heavy chain: produces motor activity with actin filaments; isoform B2 may play a role in neuronal cell motility [RGD, Feb 2006]

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