Ppt2 (NM_019367) Rat Tagged ORF Clone

CAT#: RR206591

  • TrueORF®

Ppt2 (Myc-DDK-tagged ORF) - Rat palmitoyl-protein thioesterase 2 (Ppt2), (10 ug)



  "NM_019367" in other vectors (3)

Need custom modification / cloning service?
Get a free quote

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms PPT-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR206591 representing NM_019367
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGGCTATGGAGGCAGAGGCTTCCTTCGGCTTGGGCTTTGCTTCTCCTGCCTTTCCTGCCGCTGC
TGTTGCCCGCAGCCCCCGCACCCCACCGCGGGTCCTACAAGCCAGTGATCGTGGTGCATGGGCTCTTTGA
CAGTTCATACAGCTTCCGCCACCTGCTGGACTACATCAATGAGACACACCCCGGGACTGTGGTGACAGTG
CTTGATCTCTTCGATGGCAGAGAGAGCTTGCGACCCCTGTGGGAACAGGTTCAAGGGTTCCGAGAGGCTG
TGGTCCCCATCATGGAAAAGGCCCCTGAGGGGGTACACCTCATCTGCTACTCCCAGGGAGGCCTGGTGTG
CCGCGCATTGCTGTCTGTCATGGATGAGCACAATGTGGATTCCTTCATCTCCCTTTCTTCTCCACAGATG
GGACAGTATGGAGACACGGACTATTTGAAGTGGCTGTTCCCTACGTCCATGCGGTCTAACCTCTATCGGA
TCTGCTATAGCCCCTGGGGCCAGGAATTTTCCATTTGCAACTACTGGCACGACCCTCACCACGATGACTT
GTACCTCAACGCCAGCAGCTTTCTGGCCCTCATCAATGGGGAGAGAGACCATCCGAATGCCACTGCATGG
CGGAAGAACTTCCTTCGTGTGGGCCGTCTGGTGCTGATTGGGGGTCCGGACGATGGAGTCATCACTCCCT
GGCAATCTAGCTTCTTTGGTTTCTATGATGCCAACGAGACGGTCTTGGAGATGGAGGAACAGCCGGTGTA
TCTTCGAGACTCTTTTGGGTTGAAGACTCTCTTGGCCCGGGGGGCCATAGTGAGATGCCCCATGGCTGGG
GTCTCTCACACCACCTGGCACTCCAATCGCACGCTCTATGACGCTTGCATTGAGCCCTGGCTCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR206591 representing NM_019367
Red=Cloning site Green=Tags(s)

MPGLWRQRLPSAWALLLLPFLPLLLPAAPAPHRGSYKPVIVVHGLFDSSYSFRHLLDYINETHPGTVVTV
LDLFDGRESLRPLWEQVQGFREAVVPIMEKAPEGVHLICYSQGGLVCRALLSVMDEHNVDSFISLSSPQM
GQYGDTDYLKWLFPTSMRSNLYRICYSPWGQEFSICNYWHDPHHDDLYLNASSFLALINGERDHPNATAW
RKNFLRVGRLVLIGGPDDGVITPWQSSFFGFYDANETVLEMEEQPVYLRDSFGLKTLLARGAIVRCPMAG
VSHTTWHSNRTLYDACIEPWLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019367
ORF Size 906 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019367.2, NP_062240.1
RefSeq Size 1719 bp
RefSeq ORF 909 bp
Locus ID 54398
UniProt ID O70489
MW 34.4 kDa
Gene Summary human homolog catalyzes the removal of long chain fatty acyl groups from modified cysteine residues [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

Other Versions

Customer Reviews 
Loading...