United States
Germany
Japan
United Kingdom
China
Acsl4 (NM_053623) Rat Tagged ORF Clone
CAT＃: RR205894
- TrueORF®
Acsl4 (Myc-DDK-tagged ORF) - Rat acyl-CoA synthetase long-chain family member 4 (Acsl4), (10 ug)
"NM_053623" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Acs4; Facl4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205894 representing NM_053623
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCCGGAAGTCCATATCGCTCTGTCACGCACT TCGACTCACTAGCTGTCATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTAGC CAAGTTTGGGAAGAAGGACAGCCTGGGGACCCGGGAGATCCTGAGCGAAGAAAATGAAATGCAGCCAAAT GGAAAGGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATAAACTATCTTGAAGTGAACTGCCGAG TGAATAACTTTGGAAGTGGCCTCACTGCACTGGGACTGAAACCAAAGAACACCATTGCCATTTTCTGTGA GACCAGGGCGGAGTGGATGATTGCAGCGCAGACCTGCTTTAAGTACAACTTTCCGCTTGTGACTTTATAT GCTACCCTTGGCAAAGAAGCTGTCGTTCATGGATTAAATGAGTCTGAGGCTTCCTATCTGATTACTAGTG TTGAACTTCTGGAAAGCAAACTGAAGGCGGCATTATTAGATATCAATTGTGTTAAACATATCATTTATGT GGATAACAAGACTATCAATAGAGCGGAGTACCCCGAGGGGCTTGAGATTCACAGCATGCAGTCAGTAGAG GAGCTGGGATCCAAGCCAGAAAACTCAAGCATTCCTCCAAGTAGACCAACTCCTTCGGACATGGCCATTG TCATGTACACCAGTGGCTCTACTGGCCGCCCCAAGGGAGTGATGATGCACCATAGCAATCTGATAGCTGG GATGACAGGCCAGTGTGAGCGCATACCTGGATTAGGACCAAAGGACACATATATTGGCTACTTACCTTTG GCTCATGTGCTGGAACTGACAGCAGAGATCTCCTGCTTTACCTACGGCTGTAGGATTGGATATTCGTCAC CACTCACACTGTCTGACCAGTCCAGCAAAATCAAGAAGGGAAGCAAGGGTGATTGTACTGTACTGAAACC TACACTTATGGCGGCCGTTCCGGAAATCATGGATAGAATTTACAAGAATGTTATGAGCAAGGTTCAAGAG ATGAATTATATTCAGAAGACTCTATTTAAAATTGGGTATGATTATAAATTAGAGCAAATCAAGAAAGGAT ATGATGCCCCTCTTTGTAATCTGATACTGTTTAAGAAGGTGAAGGCTCTTCTAGGAGGAAATGTCCGCAT GATGCTGTCCGGCGGTGCCCCACTGTCCCCCCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCC ATTGGTCAGGGCTATGGGCTGACAGAATCATGCGGTGCTGGAACAGTTACTGAAGTTACCGACTACACTA CTGGAAGAGTTGGCGCTCCTCTTATTTGCTGTGAAATTAAACTGAAGGACTGGCAGGAAGGTGGTTATAC AGTTCATGATAAGCCAAACCCCAGAGGTGAAATTGTGATTGGAGGCCAGAATATCTCCATGGGATATTTT AAAAATGAAGAGAAAACAGCAGAAGATTATTCTGTGGATGAAAATGGACAAAGGTGGTTTTGCACTGGTG ATATTGGAGAATTCCACCCTGATGGATGCTTACAGATTATCGATCGTAAGAAAGATCTGGTGAAGCTGCA AGCCGGAGAGTACGTGTCTCTTGGGAAAGTCGAAGCCGCACTGAAGAATTGTCCACTGATCGACAACATC TGTGCTTTTGCCAAAAGTGACCAGTCTTATGTGATCAGTTTTGTGGTTCCCAACCAGAAAAAGTTGACTC TTTTGGCACAACAGAAGGGGGTAGAAGGATCTTGGGTTGATATTTGCAATAATCCTGCCATGGAAGCTGA AATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAACGATTTGAAATTCCAATCAAAGTT CGATTGAGCCCAGAGCCATGGACCCCTGAAACTGGTTTGGTAACAGATGCCTTCAAGCTGAAAAGGAAGG AACTGAAGAACCATTATCTTAAAGACATTGAGCGGATGTATGGGGGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205894 representing NM_053623
Red=Cloning site Green=Tags(s) MAKRIKAKPTSDKPGSPYRSVTHFDSLAVIDIPGADTLDKLFDHAVAKFGKKDSLGTREILSEENEMQPN GKVFKKLILGNYKWINYLEVNCRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLY ATLGKEAVVHGLNESEASYLITSVELLESKLKAALLDINCVKHIIYVDNKTINRAEYPEGLEIHSMQSVE ELGSKPENSSIPPSRPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPL AHVLELTAEISCFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQE MNYIQKTLFKIGYDYKLEQIKKGYDAPLCNLILFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCP IGQGYGLTESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDWQEGGYTVHDKPNPRGEIVIGGQNISMGYF KNEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNI CAFAKSDQSYVISFVVPNQKKLTLLAQQKGVEGSWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKV RLSPEPWTPETGLVTDAFKLKRKELKNHYLKDIERMYGGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053623 |
| ORF Size | 2010 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053623.1, NP_446075.1 |
| RefSeq Size | 4862 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 113976 |
| UniProt ID | O35547 |
| MW | 74.3 kDa |
| Gene Summary | catalyzes the ligation of a fatty acid to produce acyl Co-A; acts on a wide range of fatty acids with preferential activity for arachidonate and eicosapentaenoate; plays a role in fatty acid metabolism [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205894 | Acsl4 (untagged ORF) - Rat acyl-CoA synthetase long-chain family member 4 (Acsl4), (10 ug) |
CNY 7,368.00 |
|
| RR205894L3 | Lenti ORF clone of Acsl4 (Myc-DDK-tagged ORF) - Rat acyl-CoA synthetase long-chain family member 4 (Acsl4), (10 ug) |
CNY 10,450.00 |
|
| RR205894L4 | Lenti ORF clone of Acsl4 (mGFP-tagged ORF) - Rat acyl-CoA synthetase long-chain family member 4 (Acsl4), (10 ug) |
CNY 11,500.00 |
