Cacna1c (NM_012517) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RATIVS302
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205823 representing NM_012517 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGGGCCTTCGCTCAGCCATCCACACCCCCATACCAGCCGCTCTCCAGTTGCCTGTCTGAGGACA CAGAGAGGAAGTTCAAGGGCAAGGTGGTACACGAAGCTCAACTCAACTGTTTCTACATTTCTCCTGGAGG TTCCAACTATGGGAGCCCACGCCCAGCTCATGCCAACATGAATGCCAATGCAGCTGCAGGACTTGCCCCC GAGCACATCCCTACTCCAGGGGCAGCACTGTCCTGGCAGGCAGCCATCGATGCCGCCCGGCAGGCCAAGT TAATGGGCAGTGCTGGCAATGCGACCATCTCTACCGTCAGTTCCACACAGCGGAAGCGGCAGCAGTATGG GAAACCCAAGAAGCAGGGGGGCACAACTGCCACACGGCCGCCCCGGGCTCTGCTCTGCCTGACTCTGAAG AACCCCATCAGGAGGGCATGCATCAGCATTGTTGAATGGAAATCATTTGAAATAATTATTTTATTGACGA TTTTTGCCAATTGTGTGGCCTTAGCAATCTATATTCCCTTTCCGGAAGATGACTCCAACGCCACCAACTC CAACCTGGAACGGGTGGAGTATCTCTTCCTCATCATTTTTACCGTGGAAGCATTTTTAAAAGTGATTGCC TACGGACTACTCTTCCACCCCAACGCTTACCTCCGCAACGGTTGGAATTTACTAGATTTTATAATCGTGG TTGTAGGGCTTTTTAGTGCAATTTTAGAACAAGCAACCAAAGCTGACGGGGCCAACGCTCTGGGAGGGAA AGGAGCGGGATTCGATGTGAAGGCACTGAGAGCTTTCCGTGTGCTTCGTCCCCTGCGGCTGGTGTCTGGA GTCCCAAGTCTCCAGGTGGTCCTGAACTCCATCATCAAGGCCATGGTGCCCCTGCTGCACATTGCCCTCC TCGTGCTCTTCGTCATCATCATCTATGCCATTATCGGCCTGGAGCTCTTCATGGGGAAGATGCACAAGAC CTGCTACAACCAGGAGGGCATAATAGATGTTCCAGCGGAAGAGGATCCCTCCCCTTGTGCTTTGGAGACA GGCCATGGGCGACAGTGTCAGAACGGGACCGTGTGCAAGCCCGGGTGGGATGGGCCCAAGCACGGCATCA CCAACTTCGACAACTTCGCCTTCGCCATGCTGACGGTGTTCCAGTGTATCACCATGGAGGGCTGGACAGA CGTGCTGTACTGGATGCAAGACGCTATGGGCTATGAGTTGCCCTGGGTGTATTTTGTCAGTCTGGTCATC TTTGGATCCTTTTTCGTTCTAAATCTGGTTCTCGGTGTTTTGAGCGGAGAGTTTTCCAAAGAGAGGGAGA AAGCCAAAGCTCGAGGAGACTTCCAGAAGCTTCGTGAGAAGCAGCAGCTAGAAGAAGATCTCAAAGGCTA CCTGGACTGGATCACCCAGGCGGAAGACATAGACCCTGAGAATGAGGACGAGGGCATGGATGAAGACAAA CCCCGAAACATGAGCATGCCCACAAGTGAGACTGAGTCTGTCAACACCGAAAACGTGGCTGGAGGTGACA TCGAGGGTGAAAACTGTGGAGCCCGGCTTGCCCACCGGATCTCCAAATCCAAGTTCAGCCGCTACTGGCG CCGGTGGAATAGATTCTGCAGAAGAAAGTGCCGTGCCGCAGTTAAGTCCAATGTCTTCTACTGGCTGGTG ATCTTCCTGGTGTTCCTCAACACCCTCACCATTGCCTCCGAACACTACAACCAGCCTCATTGGCTCACAG AAGTGCAAGACACGGCCAACAAGGCCCTCCTGGCCCTTTTCACGGCAGAAATGCTCCTGAAGATGTACAG CCTGGGTCTGCAGGCCTACTTTGTATCCCTCTTCAACCGCTTTGACTGTTTCATTGTGTGCGGGGGCATC CTGGAGACCATCCTGGTGGAGACGAAGATCATGTCGCCCCTGGGCATCTCTGTGCTGAGATGTGTGCGGC TGCTCAGGATTTTCAAGATCACCAGGTACTGGAACTCCCTGAGCAACCTCGTGGCGTCCTTGCTGAACTC AGTGCGCTCCATCGCCTCCCTGCTGCTACTCCTCTTCCTCTTCATCATCATCTTCTCCCTCCTGGGGATG CAGCTGTTTGGTGGAAAGTTCAACTTTGATGAGATGCAGACCCGTAGGAGCACGTTTGATAACTTCCCAC AGTCTCTCCTCACTGTGTTTCAGATCCTGACCGGGGAGGACTGGAATTCGGTGATGTATGATGGGATCAT GGCTTATGGCGGCCCCTCTTTTCCAGGGATGTTAGTCTGTATTTACTTCATCATCCTCTTCATCTGTGGA AATTATATCCTACTGAATGTGTTCTTGGCCATTGCGGTGGACAACCTGGCTGATGCGGAGAGCCTGACCT CTGCCCAAAAAGAGGAAGAAGAAGAGAAGGAGAGAAAGAAGCTGGCCAGGACTGCCAGCCCAGAAAAGAA ACAGGAGGTGATGGAGAAACCAGCAGTAGAGGAGAGCAAAGAGGAGAAAATTGAGCTGAAGTCCATTACA GCAGATGGAGAATCCCCACCCACTACCAAGATCAACATGGATGACCTCCAGCCCAGTGAAAATGAGGACA AGAGTCCCCACTCCAACCCAGACACTGCAGGTGAAGAGGATGAGGAGGAGCCCGAGATGCCTGTGGGCCC ACGCCCCCGGCCCCTGTCTGAGCTGCACCTTAAGGAAAAGGCAGTCCCCATGCCGGAAGCCAGTGCATTT TTCATCTTCAGCCCAAACAACAGGTTCCGCCTGCAGTGCCACCGCATTGTCAATGACACGATCTTCACCA ACCTCATCCTCTTCTTCATTCTGCTCAGTAGCATCTCCCTGGCGGCTGAGGACCCCGTCCAGCACACCTC CTTCAGGAACCACATTCTGTTTTATTTTGACATTGTTTTTACCACCATTTTCACCATTGAAATTGCTCTA AAGATGACTGCTTACGGGGCTTTCCTGCACAAGGGCTCTTTCTGCCGAAATTACTTCAATATCCTGGACC TGCTGGTGGTTAGCGTGTCCCTCATCTCCTTTGGCATTCAGTCCAGCGCGATCAACGTTGTGAAGATTTT AAGAGTGCTTCGTGTCCTCAGGCCCCTGAGGGCCATCAACAGGGCCAAGGGGCTAAAGCACGTCGTTCAG TGTGTGTTTGTGGCCATCCGGACCATTGGAAACATTGTAATTGTCACCACTCTGCTGCAGTTCATGTTCG CCTGCATTGGGGTCCAGCTCTTCAAGGGAAAGCTCTATACCTGTTCGGATAGTTCCAAACAGACGGAGGC AGAATGCAAGGGTAACTATATAACATACAAAGACGGAGAAGTTGACCACCCCATTATCCAGCCTCGAAGT TGGGAGAACAGCAAGTTCGACTTTGACAATGTTCTGGCAGCCATGATGGCCCTCTTTACCGTCTCCACCT TCGAGGGGTGGCCAGAGCTGCTGTACCGCTCCATTGACTCCCACACAGAAGACAAGGGTCCCATCTACAA CTATCGTGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATCATTGCCTTCTTCATGATGAACATC TTCGTGGGTTTCGTCATTGTCACCTTCCAGGAGCAGGGGGAACAAGAATACAAGAACTGTGAGCTGGACA AGAACCAGAGACAATGTGTGGAATATGCCCTCAAGGCCCGACCCTTGCGAAGGTACATCCCCAAGAACCA GCACCAGTACAAAGTGTGGTACGTGGTCAACTCCACCTACTTCGAGTATCTGATGTTCGTCCTCATCCTG CTCAACACCATCTGCCTGGCCATGCAGCACTACGGCCAGAGCTGCCTCTTCAAAATCGCCATGAATATAC TCAACATGCTTTTCACTGGCCTCTTCACGGTGGAGATGATCCTGAAGCTCATTGCCTTCAAACCCAAGCA CTATTTCTGTGATGCATGGAATACATTTGACGCCTTGATTGTTGTGGGTAGCATTGTTGATATAGCAATC ACCGAGGTACACCCAGCTGAACATACCCAATGCTCTCCCTCTATGAGTGCAGAGGAGAACTCCCGCATCT CCATCACCTTCTTCCGCCTCTTCCGGGTCATGCGCCTGGTGAAGCTGCTGAGCCGAGGGGAAGGCATCCG GACCCTGCTGTGGACCTTCATCAAGTCCTTCCAGGCCCTGCCCTATGTGGCCCTTTTGATTGTGATGCTG TTCTTTATCTATGCAGTGATTGGGATGCAGGTATTTGGGAAGATTGCCCTGAATGACACCACAGAGATCA ATCGGAACAACAACTTCCAGACGTTCCCTCAGGCTGTGCTACTGCTCTTCAGGTGCGCCACTGGGGAGGC CTGGCAGGATATCATGCTAGCCTGTATGCCAGGCAAGAAGTGTGCTCCAGAGTCTGAGCCCAGCAACAGC ACGGAAGGGGAGACACCCTGTGGCAGCAGTTTCGCTGTCTTCTACTTCATCAGCTTCTACATGCTCTGTG CCTTCCTGATCATCAACCTCTTTGTAGCTGTTATCATGGACAACTTTGACTACCTGACTAGGGATTGGTC TATCCTTGGTCCCCATCACCTGGATGAATTCAAGAGAATCTGGGCCGAATATGACCCTGAAGCCAAGGGT CGGATCAAACACTTGGATGTGGTGACCCTCCTCCGTCGAATTCAGCCCCCACTGGGTTTTGGGAAGTTGT GTCCTCACCGTGTGGCCTGCAAACGCCTGGTGTCCATGAACATGCCTCTGAACAGTGATGGGACGGTCAT GTTCAATGCTACACTGTTTGCCCTTGTCAGGACAGCCCTGAGGATCAAAACAGAAGGGAACCTAGAGCAA GCCAATGAGGAGCTGAGAGCCATCATCAAGAAAATCTGGAAGAGGACCAGCATGAAGCTGTTGGACCAGG TGGTGCCCCCTGCAGGTGATGACGAGGTCACAGTGGGCAAGTTCTATGCCACCTTCCTGATCCAAGAGTA CTTCAGGAAATTCAAGAAGCGAAAAGAGCAGGGGCTGGTCGGCAAGCCCTCGCAGAGGAATGCACTGTCT CTGCAGGCTGGCTTACGCACCTTGCATGACATTGGGCCTGAGATCCGGAGAGCCATCTCTGGGGATCTGA CGGCTGAGGAGGAGCTGGACAAGGCTATGAAGGAGGCAGTGTCTGCTGCCTCCGAAGACGACATCTTCAG GAGGGCTGGAGGCCTGTTTGGCAACCACGTCAGCTACTACCAGAGTGACAGCAGGAGCAACTTCCCTCAG ACGTTTGCCACCCAACGCCCACTGCACATCAACAAGACAGGGAACAACCAAGCGGACACCGAATCACCGT CCCATGAGAAGCTGGTGGACTCCACTTTCACCCCCAGCAGCTACTCATCCACGGGCTCCAATGCCAACAT CAACAATGCCAACAACACTGCCCTGGGCCGCTTCCCCCACCCTGCTGGCTACTCCAGCACGGTCAGCACT GTGGAGGGCCATGGGCCTCCCTTGTCCCCTGCTGTCCGGGTACAGGAGGCAGCATGGAAACTCAGCTCTA AGAGGTGCCACTCCCGAGAGAGCCAGGGGGCCACGGTGAGTCAGGATATGTTTCCAGATGAGACCCGCAG CAGCGTGAGGCTGAGCGAAGAAGTTGAGTACTGCAGTGAGCCCAGCCTGCTCTCCACAGATATACTCTCC TACCAGGACGATGAAAACCGACAACTGACCTGTCTAGAGGAGGACAAGAGGGAGATCCAGCCATCTCCGA AGAGGAGTTTCCTTCGCTCTGCCTCTCTAGGTCGAAGGGCCTCCTTCCATCTGGAATGTCTAAAGCGACA AAAGGATCAAGGGGGAGACATCTCTCAGAAGACAGCCTTGCCCTTGCATCTGGTTCATCACCAGGCATTG GCAGTGGCAGGCTTGAGCCCCCTCCTGCAGAGAAGCCATTCTCCTTCCACGTTCCCCAGGCCACGCCCCA CGCCCCCTGTCACTCCAGGCAGCCGGGGCAGGCCCCTACAGCCCATCCCTACCCTGCGGCTGGAGGGGGC GGAGTCCAGTGAGAAACTCAACAGCAGCTTCCCGTCCATCCACTGCAGCTCCTGGTCAGAGGAGACCACA GCCTGTAGTGGGGGCAGCAGCATGGCCCGGAGAGCCCGGCCCGTCTCCCTCACCGTGCCCAGCCAGGCTG GAGCTCCAGGCAGACAGTTCCACGGCAGCGCCAGCAGCCTGGTGGAAGCGGTCTTGATTTCAGAAGGACT GGGACAGTTTGCTCAAGATCCCAAGTTCATCGAGGTCACCACACAGGAGCTGGCTGACGCCTGCGATATG ACAATAGAGGAGATGGAGAACGCCGCAGACAACATCCTCAGCGGGGGCGCCCAGCAGAGCCCCAACGGCA CCCTCTTACCTTTTGTGAACTGCAGGGACCCAGGGCAGGACAGGGCTGTGGTCCCAGAGGACGAGAGCTG TGTATATGCCCTGGGGCGAGGCCGGAGCGAGGAAGCGCTCCCGGACAGCAGGTCCTATGTCAGCAACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205823 representing NM_012517 Red=Cloning site Green=Tags(s) MIRAFAQPSTPPYQPLSSCLSEDTERKFKGKVVHEAQLNCFYISPGGSNYGSPRPAHANMNANAAAGLAP EHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGGTTATRPPRALLCLTLK NPIRRACISIVEWKSFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIA YGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSG VPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALET GHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVI FGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEDK PRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLV IFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGI LETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGM QLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICG NYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSIT ADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAF FIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIAL KMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQ CVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRS WENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNI FVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLIL LNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMILKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAI TEVHPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVML FFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNS TEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKG RIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQ ANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALS LQAGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQSDSRSNFPQ TFATQRPLHINKTGNNQADTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRFPHPAGYSSTVST VEGHGPPLSPAVRVQEAAWKLSSKRCHSRESQGATVSQDMFPDETRSSVRLSEEVEYCSEPSLLSTDILS YQDDENRQLTCLEEDKREIQPSPKRSFLRSASLGRRASFHLECLKRQKDQGGDISQKTALPLHLVHHQAL AVAGLSPLLQRSHSPSTFPRPRPTPPVTPGSRGRPLQPIPTLRLEGAESSEKLNSSFPSIHCSSWSEETT ACSGGSSMARRARPVSLTVPSQAGAPGRQFHGSASSLVEAVLISEGLGQFAQDPKFIEVTTQELADACDM TIEEMENAADNILSGGAQQSPNGTLLPFVNCRDPGQDRAVVPEDESCVYALGRGRSEEALPDSRSYVSNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_012517
ORF Size	6510 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012517.2, NP_036649.2
RefSeq Size	8257 bp
RefSeq ORF	6513 bp
Locus ID	24239
UniProt ID	P22002
MW	243.5 kDa
Gene Summary	subunit of an L-type calcium channel that may be involved in bone metabolism and compensatory renal growth [RGD, Feb 2006]

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