Nrxn3 (NM_053817) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204746 representing NM_053817 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTTTACCCTCCACTCAGTTTTCTTCACCCTGAAGGTGAGCAGCTTTCTGGGCTCCCTGGTGGGGC TCTGCCTGGGTCTGGAGTTCATGGGCCTCCCTAACCAGTGGGCCCGCTATCTACGCTGGGATGCTAGCAC GCGCAGTGACCTGAGCTTCCAGTTCAAGACCAATGTTTCCACTGGGCTGCTCCTGTATTTGGATGATGGT GGTGTCTGTGACTTCCTCTGCCTCTCCCTGGTGGATGGCCGTGTTCAGCTCCGCTTTAGCATGGACTGCG CCGAGACCACTGTGTTGTCCAACAAGCAGGTGAACGACAGCAGTTGGCACTTCCTCATGGTGAGCCGTGA CCGTGTGCGCACTGGGCTGGTGATTGATGGTGAGGGCCAGTCTGGTGAGCTACGGGCCCAGCGGCCCTAC ATGGATGTGGTCAGTGATTTGTTCCTCGGTGGCGGGCCCGCTGACATTCGACCTTCTGCCCTGACCCTTG ATGGAGTACAGAACATGCCTGGCTTTAAGGGATTAATGCTGGATCTCAAGTATGGCAACTCGGAACCTCG GCTTCTCGGGAGCCAGAGTGTCCAGTTAGAAGCAGAGGGACCCTGTGGCGAGCGTCCCTGTGAAAATGGT GGGATCTGCTTCCTCTTGGATGGTCATCCCACCTGTGACTGTTCTACCACTGGCTATGGCGGCACACTCT GCTCAGAAGATGTCAGTCAAGGTCCAGGCCTCTCCCATCTTATGATGAGTGAACAAGGTAGAAGTAAAGC TCGAGAGGAGAACGTGGCCACCTTCCGAGGCTCAGAGTATCTGTCCTATGACCTGTCCCAGAACCCCATC CAGAGCAGCAGCAGTGAAATCACCCTTTCCTTTAAGACCTGGCAACGCAATGGGCTCATCCTCCACACGG GCAAGTCGGCCGACTATGTTAACCTGGCTCTGAAGGATGGTGCGGTCTCCTTGGTCATTAACCTGGGGTC CGGGGCCTTTGAGGCCATTGTGGAGCCAGTGAATGGGAAATTCAACGACAACGCCTGGCATGATGTCAAA GTGACACGCAATCTTCGGCAGGTGACAATCTCTGTTGATGGCATCCTAACAACAACAGGCTACACTCAAG AGGACTACACCATGCTGGGCTCAGATGACTCCTCCTATGTTGGGCCCAGCCCAAGCACTGCGGACTTGCC AGGCTCACCTGTGAGCAACAACTTCATGGGCTGCCTTAAAGAGGTTGTTTATAAGAATAATGACATCCGT CTGGAGCTGTCTCGCTTGGCCCGGATTGGGGCCACCAAGATGAAAATCTATGGTGAAGTTGTGTTCAAGT GTGAGAACGTGGCCACTCTGGACCCCATCAACTTTGAGACCCCAGAGGCTTACATCAGCTTGCCCAAGTG GAACACCAAACGTATGGGTTCCATCTCCTTTGACTTTCGCACCACTGAGCCCAACGGTCTGATCCTCTTA ACTCACGGGAAACCCCAAGAAAGGAAAGACGTCCGGAGCCAGAAGAACACAAAAGTTGACTTCTTTGCTG TGGAACTTCTTGATGGCAACCTGTATTTGCTGCTTGACATGGGCTCTGGCACCATCAAAGTGAAGGCCAC TCAGAAGAAAGCCAATGATGGGGAATGGTACCACGTGGACATTCAGCGGGATGGCAGATCAGGTACCATA TCTGTGAACAGCAGGCGCACACCGTTCACCGCCAGTGGGCAAAGTGAAATCTTGGACCTGGAGGGGGACA TGTATCTGGGCGGACTGCCAGAGAACCGGGCTGGCCTCATTCTCCCCACGGAGCTCTGGACTGCCATGCT CAACTACGGTTATGTGGGCTGCATCCGCGACCTGTTCATCGATGGGCGCAGCAAAAACATCCGGCAGCTG GCGGAGATGCAGAATGCAGCAGGCGTCAAGTCCTCCTGTTCACGCATGAGCGCCAAGCAGTGTGACAGCT ACCCCTGCAAGAACAATGCGGTGTGCAAGGACGGCTGGAACCGCTTCATCTGCGACTGCACTGGCACCGG CTACTGGGGTAGAACCTGTGAAAGAGAGGCATCCATCCTGAGCTATGACGGCAGCATGTATATGAAGGTC ATCATGCCCATGGTGATGCACACAGAGGCAGAGGATGTTTCCTTCCGGTTCATGTCCCAGCGAGCCTATG GGCTACTGGTAGCGACTACCTCCAGGGATTCTGCAGATACACTGCGTCTAGAGTTGGATGGTGGGCGTGT CAAGCTCATGGTTAACTTAGACTGTATCAGGATAAACTGTAACTCCAGCAAAGGACCTGAGACCCTGTAC GCAGGGCAGAAGCTCAATGACAATGAGTGGCATACCGTCCGGGTAGTGCGAAGAGGAAAAAGCCTTAAAC TAACAGTGGACGATGATGTGGCTGAGGGTACGATGGTGGGCGACCACACCCGCCTGGAGTTCCACAATAT TGAGACTGGTATCATGACGGAGAAGCGTTACATCTCCGTGGTCCCCTCCAGTTTCATCGGCCATCTACAG AGCCTCATGTTCAATGGCTTGCTGTACATCGATTTGTGCAAAAATGGTGACATCGACTACTGCGAACTGA AGGCTCGTTTTGGACTGAGAAACATCATCGCTGACCCTGTCACTTTTAAGACCAAGAGCAGCTACCTGAC CCTTGCCACCCTCCAGGCTTACACTTCCATGCATCTCTTTTTCCAGTTCAAGACCACTTCAGCTGATGGC TTCATTCTCTTCAACAGTGGTGATGGCAATAATTTTATTGCAGTTGAATTGGTCAAGGGGTACATACACT ATGTGTTTGATCTTGGCAATGGTCCCAATGTGATCAAAGGCAACAGTGACCGTCCTCTGAATGACAACCA GTGGCACAACGTTGTCATCACTCGGGACAACAGTAATACCCACAGCCTGAAAGTGGACACCAAGGTAGTC ACTCAGGTCATCAATGGTGCCAAGAATCTGGATTTGAAAGGTGACCTCTATATGGCTGGCTTAGCCCAGG GCATGTATAGCAACCTTCCAAAGCTCGTGGCCTCTCGGGATGGATTTCAGGGTTGCCTGGCCTCTGTGGA CTTGAATGGGCGCCTGCCTGATCTCATCAACGATGCTCTCCACAGGAGTGGACAGATTGATCGAGGCTGT GAAGGACCCAGTACAACCTGTCAGGAGGATTCATGTGCCAACCAGGGGGTTTGCATGCAGCAGTGGGAAG GTTTTACATGTGACTGCTCCATGACATCATATTCTGGAAACCAGTGCAATGACCCTGGTGCCACATATAT CTTTGGGAAAAGCGGTGGTCTCATCCTCTACACCTGGCCAGCAAATGACAGACCCAGCACACGCTCTGAC CGCCTCGCCGTGGGATTCAGCACCACTGTGAAGGATGGTGTCTTAGTACGCATCGACAGTGCCCCTGGAC TTGGCGACTTTCTCCAGCTTCACATAGAACAAGGGAAAATTGGAGTTGTCTTCAATATTGGCACAGTTGA CATCTCCATCAAAGAAGAGAGAACCCCTGTCAACGATGGCAAATACCATGTGGTGCGCTTCACCAGAAAT GGGGCCAATGCAACCCTTCAGGTGGACAACTGGCCAGTGAATGAGCATTATCCTACAGGCAACACTGATA ATGAACGCCGCCAAATGGTAAAACAGAAAATCCCCTTCAAATATAATCGGCCTGTAGAGGAGTGGCTGCA GGAAAAAGGCCGGCAGCTAACCATCTTCAACACCCAGGCGCAAATAGCCATCGGAGGAAAGGACAAAGGA CGTCTCTTCCAAGGCCAACTCTCTGGGCTCTATTATGATGGTTTGAAAGTACTGAACATGGCGGCTGAGA ACAACCCTAATATTAAAATCAATGGAAGTGTCCGGCTAGTGGGAGAAGTCCCATCAGTCTCGGGAACAAC ACATACAACCTCCATGCCACCTGAAATGTCTACCACTGTCATGGAAACCACCACCACGATGGCTACGACC ACCACCCGCAAAAACCGCTCTACAGCCAGCATTCAGCCAACATCAGATGACCTTGTTTCATCTGCTGAAT GCTCAAGTGATGATGAAGACTTTGTCGAGTGTGAACCAAGTACAGGTAGGTCAGATAAGAGTCTTTCCAC TTCAATCTTCGAAGGTGGCTACAAAGCGCATGCGCCCAAGTGGGAATCCAAGGACTTTAGACCTAACAAA GTCTCGGAAACTAGTAGAACTACAACCACCTCTTTGTCCCCTGAGCTGATCCGCTTCACAGCGTCCTCCT CGTCTGGGATGGTGCCCAAATTGCCAGCTGGCAAAATGAATAACCGTGATCTCAAACCCCAGCCTGATAT AGTCTTGCTTCCGTTGCCCACTGCCTATGAGCTAGACAGCACCAAACTGAAGAGCCCACTAATTACTTGC CCCATGTTCCGTAATGTGCCCACAGCAAACCCCACGGAGCCAGGAATCAGACGGGTTCCGGGGGCCTCAG AGGTGATCCGGGAGTCCAACAGTACAACAGGGATGGTCGTCGGCATTGTGGCTGCTGCCGCCCTCTGCAT CTTGATCCTCCTGTACGCCATGTACAAGTACAGGAACAGGGACGAGGGGTCCTATCAAGTGGACGAGACG AGGAACTACATCAGCAACTCGGCCCAGAGCAACGGCACGCTCATGAAGGAGAAACAAGCCAGCTCCAAGA GCGGCCATAAGAAACAGAAAAACAAGGACAAGGAGTATTATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204746 representing NM_053817 Red=Cloning site Green=Tags(s) MSFTLHSVFFTLKVSSFLGSLVGLCLGLEFMGLPNQWARYLRWDASTRSDLSFQFKTNVSTGLLLYLDDG GVCDFLCLSLVDGRVQLRFSMDCAETTVLSNKQVNDSSWHFLMVSRDRVRTGLVIDGEGQSGELRAQRPY MDVVSDLFLGGGPADIRPSALTLDGVQNMPGFKGLMLDLKYGNSEPRLLGSQSVQLEAEGPCGERPCENG GICFLLDGHPTCDCSTTGYGGTLCSEDVSQGPGLSHLMMSEQGRSKAREENVATFRGSEYLSYDLSQNPI QSSSSEITLSFKTWQRNGLILHTGKSADYVNLALKDGAVSLVINLGSGAFEAIVEPVNGKFNDNAWHDVK VTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDSSYVGPSPSTADLPGSPVSNNFMGCLKEVVYKNNDIR LELSRLARIGATKMKIYGEVVFKCENVATLDPINFETPEAYISLPKWNTKRMGSISFDFRTTEPNGLILL THGKPQERKDVRSQKNTKVDFFAVELLDGNLYLLLDMGSGTIKVKATQKKANDGEWYHVDIQRDGRSGTI SVNSRRTPFTASGQSEILDLEGDMYLGGLPENRAGLILPTELWTAMLNYGYVGCIRDLFIDGRSKNIRQL AEMQNAAGVKSSCSRMSAKQCDSYPCKNNAVCKDGWNRFICDCTGTGYWGRTCEREASILSYDGSMYMKV IMPMVMHTEAEDVSFRFMSQRAYGLLVATTSRDSADTLRLELDGGRVKLMVNLDCIRINCNSSKGPETLY AGQKLNDNEWHTVRVVRRGKSLKLTVDDDVAEGTMVGDHTRLEFHNIETGIMTEKRYISVVPSSFIGHLQ SLMFNGLLYIDLCKNGDIDYCELKARFGLRNIIADPVTFKTKSSYLTLATLQAYTSMHLFFQFKTTSADG FILFNSGDGNNFIAVELVKGYIHYVFDLGNGPNVIKGNSDRPLNDNQWHNVVITRDNSNTHSLKVDTKVV TQVINGAKNLDLKGDLYMAGLAQGMYSNLPKLVASRDGFQGCLASVDLNGRLPDLINDALHRSGQIDRGC EGPSTTCQEDSCANQGVCMQQWEGFTCDCSMTSYSGNQCNDPGATYIFGKSGGLILYTWPANDRPSTRSD RLAVGFSTTVKDGVLVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDISIKEERTPVNDGKYHVVRFTRN GANATLQVDNWPVNEHYPTGNTDNERRQMVKQKIPFKYNRPVEEWLQEKGRQLTIFNTQAQIAIGGKDKG RLFQGQLSGLYYDGLKVLNMAAENNPNIKINGSVRLVGEVPSVSGTTHTTSMPPEMSTTVMETTTTMATT TTRKNRSTASIQPTSDDLVSSAECSSDDEDFVECEPSTGRSDKSLSTSIFEGGYKAHAPKWESKDFRPNK VSETSRTTTTSLSPELIRFTASSSSGMVPKLPAGKMNNRDLKPQPDIVLLPLPTAYELDSTKLKSPLITC PMFRNVPTANPTEPGIRRVPGASEVIRESNSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYQVDET RNYISNSAQSNGTLMKEKQASSKSGHKKQKNKDKEYYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053817
ORF Size	4734 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053817.2, NP_446269.2
RefSeq Size	6007 bp
RefSeq ORF	4737 bp
Locus ID	116508
UniProt ID	Q07310
MW	174 kDa
Gene Summary	brain specific cell surface protein; may act as a receptor and play a role in cellular signalling [RGD, Feb 2006]

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