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Man2a1 (NM_012979) Rat Tagged ORF Clone
CAT＃: RR203565
- TrueORF®
Man2a1 (Myc-DDK-tagged ORF) - Rat mannosidase, alpha, class 2A, member 1 (Man2a1), (10 ug)
"NM_012979" in other vectors (3)
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CNY 18,530.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mana2; Manno |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203565 representing NM_012979
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTAAGCCGCCAGTTCACCGTGTTCGGCAGCGCGATCTTCTGCGTCGTAATCTTCTCACTCTACC TGATGCTGGACAGGGGTCACTTGGACTACCCTCGGGGCCCGCGCCAGGAGGGCTCCTTCCCGCAGGGCCA GCTTTCAATATTGCAAGAAAAGATCGACCATTTGGAGCGTTTGCTAGCGGAGAACAACGAGATCATCTCG AATATCAGAGACTCGGTCATCAACCTGAGTGAGTCTGTGGAAGATGGCCCACGAGGTCCAGCAGGCAACG CCAGCCAAGGCTCTGCGCACCTCCACTCTGCACAGTTGGCCCTACAGGCTGACCCCAAAGACTGCTTGTT TGCCTCGCAGAGCGGAAACCAGCACCGGGATGTGCAGATGTTGGATGTTTACGATCTGATTCCTTTTGAT AATCCAGATGGTGGAGTTTGGAAGCAAGGGTTTGACATTAAGTACGAAGCTGATGAGTGGGACCGTGAGC CCCTGCAAGTGTTTGTGGTGCCTCACTCCCATAATGACCCAGGTTGGTTGAAGACTTTCAATGACTACTT TAGAGACAAGACTCAGTATATTTTTAATAACATGGTCCTAAAGCTGAAAGAAGACTCAAGCAGGAAGTTT ATATGGTCTGAGATCTCTTACCTTGCAAAATGGTGGGATATTATAGATAATCCGAAGAAGGAAGCTGTTA AAAGTTTACTACAGAATGGTCAGCTTGAGATTGTGACCGGTGGCTGGGTGATGGCCGATGAAGCCACTAC ACATTATTTTGCCTTAATTGACCAACTGATTGAAGGGCATCAATGGCTGGAAAAAAATCTAGGAGTGAAA CCTCGATCGGGCTGGGCCATAGATCCCTTTGGACATTCACCCACAATGACTTACCTTCTAAAACGTGCTG GATTTTCACACATGCTCATCCAGAGAGTCCATTATTCGGTGAAAAAACACTTCTCTTTGCAAAAAACGCT GGAATTTTTCTGGAGACAGAATTGGGATCTTGGATCCACCACAGACATTTTGTGCCACATGATGCCCTTC TATAGCTATGACATCCCCCACACCTGTGGACCCGATCCCAAAATATGCTGCCAGTTTGATTTTAAGCGGC TTCCTGGAGGCAGATACGGTTGTCCCTGGGGAGTTCCCCCAGAAGCAATATCTCCTGGAAATGTCCAAAG CAGGGCTCAGATGCTACTGGATCAGTACCGGAAAAAGTCAAAACTTTTCCGCACCAAAGTTCTCCTGGCT CCGCTCGGAGACGATTTTCGGTTCAGTGAATACACAGAGTGGGACCTGCAGTACAGGAACTATGAGCAGT TATTCAGTTACATGAACTCTCAGCCCCATCTTAAAGTGAAGATCCAGTTTGGAACTTTGTCAGATTATTT CGATGCATTGGAGAAGTCTGTGGCAGCTGAGAAGAAGGGTGGCCAGTCTGTGTTCCCCGCCCTGAGTGGA GACTTCTTCACCTACGCTGACCGAGACGACCATTACTGGAGTGGCTACTTCACATCCAGACCTTTCTACA AACGAATGGACAGGATAATGGAATCTCGTTTAAGGACTGCTGAAATTCTTTACCATTTGGCCTTGAAACA AGCTCAGAAATACAAGATAAATAAATTTCTTTCATCACCTCATTATACAACACTGACAGAAGCCAGAAGG AACTTGGGACTGTTTCAGCATCATGATGCCATCACGGGAACCGCGAAAGACTGGGTGGTCGTGGACTATG GTACCAGACTCTTTCAGTCATTAAATTCTCTGGAGAAGATAATCGGAGATTCTGCATTTCTTCTCATTTT AAAGGACAAAAAGCTGTACCAGTCAGATCCTTCCAAAGCCTTCTTAGAGATGGACACGAAGCAAAGTTCA CAAGATTCTCTGCCCAAAAAAAATATAATACAACTGAGTGCACAGGAGCCGAGGTACCTCGTGGTCTACA ATCCCTTCGAACAAGAACGGCACTCGGTGGTGTCCGTCCGTGTGAACTCAGCCACGGTGAAAGTGCTGTC TGATCTGGGGAAAGCCGTGGAGGTTCAAGTCAGCGCAGTCTGGAAGGATATGAGAACGACTTCACAAGCA GCCTATGAGGTCGCTTTTCTAGCTCATCTGCCACCGCTGGGACTGAAAGTGTACAAGATCTTAGAATCCC AGAGTTCAAGTTCACATCTGGCTGATTATTTCCTATATAACAATGACGGGCAAGCAGAAAGTGGAATATT CCACATGAAGAACATGGTAGATTCAGGAGATGCCATCACCATAGAGAATTCCTTCCTCACACTTGGGTTT GACCGATCTGGGCTGATGGAGAAAGTGAGAAGGAAAGAAGACAATAAACAGCAGGAACTGAAGGTGCAGT TCCTGTGGTACGGAACCACCAACAAAAGGGACAAGAGCGGTGCCTACCTCTTCCTGCCTGATGGGCAGGG CCAGCCCTATGTTTCCCTGAGAACACCCTTTGTCAGAGTGACACGTGGAAGGATCTACTCAGACGTGACC TGCTTCCTTGAACACGTTACTCACAAGGTCCGGCTGTACCACATACAGGGAATAGAAGGCCAGTCCATGG AGGTTTCTAATATCGTGGACATCAGGAGTGTGCATAACCGTGAGATTGTAATGAGAATTTCCTCCAAAAT AAACAACCAAAATAGATATTATACCGACCTAAATGGATATCAGATTCAACCTAGAAGGACAATGGCCAAA CTGCCTCTTCAGGCCAATGTGTACCCGATGAGCACAATGGCCTACATCCAGGATGCTGCACATCGGCTCA CACTGCTTTCCGCTCAGTCTTTAGGCGCTTCCAGTATGGCCTCTGGTCAAATTGAAGTCTTCATGGACCG AAGACTCATGCAGGACGATAACCGTGGCCTTGGGCAAGGTGTACACGATAATAAGATTACAGCCAATTTG TTTCGAATCCTGCTAGAGAAGAGAAATGGCATGAACATGGAAGAGGACAAGAAGAGCCCTGTCAGCTACC CTTCCCTCCTCAGCCATATGACCTCGGCCTTCCTCAACCATCCCTTCCTCCCCATGGTACTAAGTGGCCA GCTCCCCTCCCCTGCCATTGAGCTGCTGAGTGAATTTCGTCTGCTGCAGTCCTCTCTCCCTTGTGATATC CATCTGGTTAATCTACGGACAATACAGTCAAAGGTGGGCAAAGGTTACTCAGATGAGGCAGCCTTGATCC TCCACAGGAAGGTGTTTGATTGCCAGTTGTCCAGCAGAGCCATGGGGCTGCCCTGTTCCACGACTCAGGG AAAGATGTCAATTCCAAAACTTTTCAACAACTTTGCTGTGGAGAGTTTCATCCCTTCCTCGCTGTCCTTG ATGCACTCGCCTCCGGACGCCCAGAACACGAGTGAAGTCAGCCTGAGTCCCATGGAGATTAGCACGTCCC GAATCCGCTTGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203565 representing NM_012979
Red=Cloning site Green=Tags(s) MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRGPRQEGSFPQGQLSILQEKIDHLERLLAENNEIIS NIRDSVINLSESVEDGPRGPAGNASQGSAHLHSAQLALQADPKDCLFASQSGNQHRDVQMLDVYDLIPFD NPDGGVWKQGFDIKYEADEWDREPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSSRKF IWSEISYLAKWWDIIDNPKKEAVKSLLQNGQLEIVTGGWVMADEATTHYFALIDQLIEGHQWLEKNLGVK PRSGWAIDPFGHSPTMTYLLKRAGFSHMLIQRVHYSVKKHFSLQKTLEFFWRQNWDLGSTTDILCHMMPF YSYDIPHTCGPDPKICCQFDFKRLPGGRYGCPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLA PLGDDFRFSEYTEWDLQYRNYEQLFSYMNSQPHLKVKIQFGTLSDYFDALEKSVAAEKKGGQSVFPALSG DFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRLRTAEILYHLALKQAQKYKINKFLSSPHYTTLTEARR NLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKIIGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSS QDSLPKKNIIQLSAQEPRYLVVYNPFEQERHSVVSVRVNSATVKVLSDLGKAVEVQVSAVWKDMRTTSQA AYEVAFLAHLPPLGLKVYKILESQSSSSHLADYFLYNNDGQAESGIFHMKNMVDSGDAITIENSFLTLGF DRSGLMEKVRRKEDNKQQELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRTPFVRVTRGRIYSDVT CFLEHVTHKVRLYHIQGIEGQSMEVSNIVDIRSVHNREIVMRISSKINNQNRYYTDLNGYQIQPRRTMAK LPLQANVYPMSTMAYIQDAAHRLTLLSAQSLGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANL FRILLEKRNGMNMEEDKKSPVSYPSLLSHMTSAFLNHPFLPMVLSGQLPSPAIELLSEFRLLQSSLPCDI HLVNLRTIQSKVGKGYSDEAALILHRKVFDCQLSSRAMGLPCSTTQGKMSIPKLFNNFAVESFIPSSLSL MHSPPDAQNTSEVSLSPMEISTSRIRLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012979 |
| ORF Size | 3444 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012979.2, NP_037111.2 |
| RefSeq Size | 6959 bp |
| RefSeq ORF | 3447 bp |
| Locus ID | 25478 |
| UniProt ID | P28494 |
| MW | 131.2 kDa |
| Gene Summary | golgi enzyme involved in asparagine-linked oligosaccharide processing [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203565 | Man2a1 (untagged ORF) - Rat mannosidase, alpha, class 2A, member 1 (Man2a1), (10 ug) |
CNY 18,530.00 |
|
| RR203565L3 | Lenti ORF clone of Man2a1 (Myc-DDK-tagged ORF) - Rat mannosidase, alpha, class 2A, member 1 (Man2a1), (10 ug) |
CNY 19,950.00 |
|
| RR203565L4 | Lenti ORF clone of Man2a1 (mGFP-tagged ORF) - Rat mannosidase, alpha, class 2A, member 1 (Man2a1), (10 ug) |
CNY 21,950.00 |
