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Prkaca (NM_001100922) Rat Tagged ORF Clone
CAT＃: RR201982
- TrueORF®
Prkaca (Myc-DDK-tagged ORF) - Rat protein kinase, cAMP-dependent, catalytic, alpha (Prkaca), (10 ug)
"NM_001100922" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cs-PKA; PKCA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201982 representing NM_001100922
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACGCCGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAGTTCCTAGCCAAAGCCA AGGAAGATTTCCTGAAAAAATGGGAGACCCCTTCTCAGAATACAGCCCAGTTGGATCATTTTGATAGAAT CAAGACCCTTGGCACCGGCTCCTTTGGGCGAGTGATGCTCGTGAAGCACAAGGAAAGTGGGAACCACTAT GCCATGAAGATCTTGGACAAGCAGAAGGTGGTGAAGCTGAAGCAGATCGAGCACACTCTGAATGAGAAGC GCATCCTGCAGGCTGTCAACTTCCCGTTCCTGGTCAAACTTGAATTCTCCTTCAAGGACAACTCAAACTT GTACATGGTCATGGAGTACGTGCCTGGTGGGGAGATGTTCTCCCACTTACGGCGGATTGGGAGGTTCAGT GAGCCCCACGCCCGTTTCTACGCGGCGCAGATCGTCTTGACTTTTGAGTATCTGCACTCCCTGGACCTCA TCTACCGGGACCTGAAGCCCGAGAACCTTCTCATCGACCAGCAGGGCTATATTCAGGTGACAGACTTCGG TTTTGCCAAGCGTGTGAAAGGCCGAACTTGGACCTTGTGTGGGACCCCCGAGTACTTGGCCCCCGAGATT ATCCTGAGCAAAGGCTACAACAAAGCTGTGGACTGGTGGGCTCTCGGGGTCCTCATCTACGAGATGGCTG CTGGTTACCCGCCCTTCTTCGCTGACCAGCCTATCCAGATCTATGAGAAAATCGTCTCTGGGAAGGTGCG GTTCCCATCCCACTTCAGCTCTGACTTGAAGGACCTGCTTCGGAACCTTCTGCAGGTGGATCTCACCAAG CGCTTTGGGAATCTCAAGAACGGGGTCAATGACATCAAGAACCACAAGTGGTTTGCCACAACTGACTGGA TCGCCATCTATCAGAGAAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGACACGAGTAA CTTTGACGACTATGAGGAGGAAGAGATCCGGGTCTCCATCAATGAGAAATGTGGCAAGGAGTTTACTGAG TTTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201982 representing NM_001100922
Red=Cloning site Green=Tags(s) MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDHFDRIKTLGTGSFGRVMLVKHKESGNHY AMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFS EPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEI ILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTK RFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTE FF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100922 |
| ORF Size | 1053 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100922.1, NP_001094392.1 |
| RefSeq Size | 2253 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 25636 |
| MW | 40.6 kDa |
| Gene Summary | active catalytic subunit of protein kinase A; phosphorylates and activates transcription factors in response to elevated levels of cAMP; mediates phosphorylation and regulation of enzyme substrates involved in intermediary metabolism [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201982 | Prkaca (untagged ORF) - Rat protein kinase, cAMP-dependent, catalytic, alpha (Prkaca), (10 ug) |
CNY 3,990.00 |
|
| RR201982L3 | Lenti ORF clone of Prkaca (Myc-DDK-tagged ORF) - Rat protein kinase, cAMP-dependent, catalytic, alpha (Prkaca), (10 ug) |
CNY 6,080.00 |
|
| RR201982L4 | Lenti ORF clone of Prkaca (mGFP-tagged ORF) - Rat protein kinase, cAMP-dependent, catalytic, alpha (Prkaca), (10 ug) |
CNY 6,650.00 |
