Ptprc (NM_138507) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CD45; L-CA; Lca; RT7; T200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200488 representing NM_138507 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTGTGGCTCAAACTTCTGGCCTTCAGCTTGGCCCTTCTCGGCCCAGAAGTCTTTGTCACAGGGC AAGGAACAACCGACGATGGACTGGACACAACAGAGATTGTTCTTCTGCCTCAAACTGACCCTTTACCTGC TCGCACCACTGAATTCACACCCCCAAGCATCTCTGAAAGAGGAAATGGCTCCTCAGAGACCACATATCTT CCAGGTTTTTCATCCACGCTGATGCCTCACCTGACCCCACAGCCTGACTCACAGACCCCCTCTGCCAGAG GAGCTGACACTCAGACACTCAGCAGCCAAGCTGACCTCACCACACTCACGGCTGCTCCCAGCGGCGAGAC TGACCCACCAGGTGTGCCCGAAGAGAGTACTGTACCGGAGACCTTTCCTGGAGGCACCCCCATCCTTGCA CGCAACAGCACTGCTCCCTCACCTACACACACTTCCAATGTCAGCACCACAGATATCTCCTCAGGTGCCA ACCTCACAACCCCTGCACCATCCACTCTGGGCTTTGCAAGCAATACCACCACAAGCACAGAAATAGCTAC CCCTCAAACGAAGCCATCATGTGATGAAAAATTTGGGAACGTTACTGTGCGTTACATCTATGATGACAGT AGTAAGAATTTTAACGCAAACCTAGAAGGTGACAAAAAACCTAAGTGTGAATATACGGATTGTGAAAAAG AGTTAAAAAATCTACCAGAATGCTCACAGAAAAACGTCACTCTCTCCAATGGCTCATGTACTCCAGATAA AATTATAAATTTAGATGTACCACCAGGGACTCACAACTTTAACTTAACAAACTGCACACCAGACATAGAA GCTAATACCTCAATTTGTTTGGAGTGGAAAATAAAAAACAAATTTACCTGCGACATTCAAAAGATTTCAT ACAATTTCCGTTGTACACCAGAGATGAAAACATTTGCTTTGGACAAACACGGAACACTTTGGTTACACAA CCTTACAGTCCGAACAAATTACACATGTGCTGCGGAAGTCCTCTACAATAACGTAATACTTTTAAAACAA GACAGAAGGGTGCAGACTGATTTTGGGACTCCAGAAATGCTTCCCCATGTTCAATGTAAGAATTCAACTA ACAGCACAACATTAGTCTCCTGGGCTGAGCCAGCATCTAAACACCATGGATACATTTTATGCTATAAAAA GACCCCTTCAGAAAAATGTGAAAATTTGGCTAATGATGTGAACAGTTTTGAGGTGAAAAACCTGAGGCCT TATACAGAGTACACAGTGTCTCTATTTGCCTATGTTATTGGGAGGGTGCAACGAAATGGCCCTGCTAAGG ATTGCAACTTTCGGACAAAAGCAGCTCGTCCAGGCAAAGTCAATGGTATGAAAACCTCCCGGGCGTCAGA CAATAGTATAAATGTCACGTGTAATTCTCCTTATGAAATTAATGGCCCTGAAGCGCGTTACATTTTGGAA GTCAAAAGTGGAGGTTCTTTAGTTAAAACTTTCAACCAATCCACATGTAAATTTGTTGTAGACAATCTCT ATTATTCAACTGACTATGAGTTTCTGGTCTATTTTTACAATGGAGAGTACCTGGGAGACCCAGAAATAAA ACCTCAATCAACATCTTATAATTCTAAAGCACTGATTATATTCCTGGTGTTTCTGATTATTGTGACATCA ATAGCCCTGCTTGTTGTTTTGTATAAAATCTATGATCTGCGTAAGAAAAGATCCAGCAATTTAGATGAAC AGCAGGAACTCGTTGAAAGGGATGAGGAGAAGCAGCTGATAAATGTGGACCCAATTCATTCTGACCTTTT GTTGGAAACATACAAAAGAAAGATTGCCGATGAGGGTAGACTGTTCCTGGCTGAATTTCAGAGCATTCCA CGGGTATTCAGCAAGTTTCCCATCAAAGATGCCCGAAAGTCCCAAAACCAGAACAAAAACCGTTATGTGG ACATTCTCCCCTATGATTACAACCGTGTGGAGCTCTCTGAAATAAATGGAGACGCAGGGTCCACCTACAT AAATGCCAGCTACATTGATGGCTTCAAGGAACCCAGGAAATACATTGCTGCACAAGGGCCCCGGGATGAG ACAGTTGATGACTTCTGGAAGATGATCTGGGAGCAGAAGGCCACAGTTATTGTCATGGTCACACGATGTG AAGAAGGAAACAGGAACAAGTGTGCAGAATATTGGCCATGCATGGAGGAAGGCACTCGGACTTTCAGAGA TGTTGTCGTGACGATCAATGACCACAAACGATGTCCTGATTACATTATCCAGAAGCTGAGCATTGCCCAC AAAAAAGAAAAAGCAACTGGAAGAGAAGTGACTCATATTCAATTCACCAGTTGGCCAGACCATGGGGTTC CTGAAGACCCTCACCTGCTCCTGAAACTTCGACGGAGAGTTAATGCTTTTAGCAACTTCTTCAGTGGACC CATTGTGGTGCACTGCAGTGCTGGCGTTGGGCGTACAGGCACCTACATTGGAATTGATGCCATGCTCGAA AGCCTAGAAGCAGAGGGCAAAGTGGATGTCTATGGCTATGTTGTCAACCTAAGGCGACAGAGATGTCTGA TGGTGCAAGTGGAGGCCCAGTACATCCTGATCCATCAGGCCTTAGTGGAGTACAATCAATTTGGGGAAAC AGAAGTGAACTTGTCTGAGTTACATTCATGTCTACAGAATCTGAAGAAGAGAGATCCACCCAGTGACCCG TCTCCTTTGGAGGCTGAGTACCAGAGACTTCCTTCATACAGGAGCTGGAGGACACAGCACATTGGAAATC AAGAAGAAAATAAAAAGAAAAACAGGAGTTCTAACGTTGTTCCATATGACTTTAACAGAGTGCCACTTAA GCATGAACTAGAGATGAGCAAAGAGAGCGAGGCTGAATCCGACGAATCTTCAGATGAGGACAGTGACTCG GAAGAAACCAGCAAATACATTAATGCGTCATTTGTGATGAGTTACTGGAAACCAGAAATGATGATTGCTG CCCAGGGTCCACTAAAAGAGACTATTGGTGACTTTTGGCAGATGATATTCCAAAGAAAAGTCAAGGTTAT TGTGATGTTGACAGAGTTAATGAGTGGAGACCAGGAAGTCTGTGCTCAATACTGGGGAGAAGGAAAGCAG ACTTATGGAGACATGGAAGTAATGTTGAAAGACACGAACAAATCCTCAGCCTATATTCTGCGAGCATTTG AACTGAGACATTCCAAGAGAAAGGAGCCTAGAACTGTGTACCAGTACCAGTGTACCACATGGAAAGGGGA GGAGCTCCCTGCAGAACCCAAAGATCTAGTGACTCTGATTCAGAACATCAAACAGAAGCTTCCCAAGAGT GGCTCAGAAGGGATGAAGTACCACAAGCATGCATCAATCCTAGTCCACTGCAGGGATGGATCCCAGCAGA CGGGGTTGTTCTGTGCTCTGTTCAATCTCCTGGAAAGTGCAGAAACAGAAGATGTGGTTGATGTTTTTCA AGTGGTAAAGTCTCTACGCAAAGCACGGCCGGGGATGGTGGGCAGCTTTGAGCAATACCAGTTCCTCTAT GACATCATGGCCAGCATCTATCCCACCCAGAATGGACAAGTGAAGAAAGCAAACAGCCAAGACAAAATTG AATTTCATAACGAAGTGGACGGAGCCAAGCAGGACGCAAACTGTGTTCAGCCAGCTGATCCTCTGAACAA AGCCCAGGAAGACAGCAAAGAAGTTGGAGCTTCAGAGCCTGCAAGTGGTTCTGAGGAGCCAGAACATTCT GCAAATGGTCCCATGAGCCCAGCTCTAACCCCGAGCTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200488 representing NM_138507 Red=Cloning site Green=Tags(s) MYLWLKLLAFSLALLGPEVFVTGQGTTDDGLDTTEIVLLPQTDPLPARTTEFTPPSISERGNGSSETTYL PGFSSTLMPHLTPQPDSQTPSARGADTQTLSSQADLTTLTAAPSGETDPPGVPEESTVPETFPGGTPILA RNSTAPSPTHTSNVSTTDISSGANLTTPAPSTLGFASNTTTSTEIATPQTKPSCDEKFGNVTVRYIYDDS SKNFNANLEGDKKPKCEYTDCEKELKNLPECSQKNVTLSNGSCTPDKIINLDVPPGTHNFNLTNCTPDIE ANTSICLEWKIKNKFTCDIQKISYNFRCTPEMKTFALDKHGTLWLHNLTVRTNYTCAAEVLYNNVILLKQ DRRVQTDFGTPEMLPHVQCKNSTNSTTLVSWAEPASKHHGYILCYKKTPSEKCENLANDVNSFEVKNLRP YTEYTVSLFAYVIGRVQRNGPAKDCNFRTKAARPGKVNGMKTSRASDNSINVTCNSPYEINGPEARYILE VKSGGSLVKTFNQSTCKFVVDNLYYSTDYEFLVYFYNGEYLGDPEIKPQSTSYNSKALIIFLVFLIIVTS IALLVVLYKIYDLRKKRSSNLDEQQELVERDEEKQLINVDPIHSDLLLETYKRKIADEGRLFLAEFQSIP RVFSKFPIKDARKSQNQNKNRYVDILPYDYNRVELSEINGDAGSTYINASYIDGFKEPRKYIAAQGPRDE TVDDFWKMIWEQKATVIVMVTRCEEGNRNKCAEYWPCMEEGTRTFRDVVVTINDHKRCPDYIIQKLSIAH KKEKATGREVTHIQFTSWPDHGVPEDPHLLLKLRRRVNAFSNFFSGPIVVHCSAGVGRTGTYIGIDAMLE SLEAEGKVDVYGYVVNLRRQRCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHSCLQNLKKRDPPSDP SPLEAEYQRLPSYRSWRTQHIGNQEENKKKNRSSNVVPYDFNRVPLKHELEMSKESEAESDESSDEDSDS EETSKYINASFVMSYWKPEMMIAAQGPLKETIGDFWQMIFQRKVKVIVMLTELMSGDQEVCAQYWGEGKQ TYGDMEVMLKDTNKSSAYILRAFELRHSKRKEPRTVYQYQCTTWKGEELPAEPKDLVTLIQNIKQKLPKS GSEGMKYHKHASILVHCRDGSQQTGLFCALFNLLESAETEDVVDVFQVVKSLRKARPGMVGSFEQYQFLY DIMASIYPTQNGQVKKANSQDKIEFHNEVDGAKQDANCVQPADPLNKAQEDSKEVGASEPASGSEEPEHS ANGPMSPALTPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_138507
ORF Size	3819 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_138507.3
RefSeq Size	5067 bp
RefSeq ORF	3822 bp
Locus ID	24699
UniProt ID	P04157
MW	143.3 kDa
Gene Summary	member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]

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