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Sh2b1 (NM_001048180) Rat Tagged ORF Clone
CAT＃: RR200245
- TrueORF®
Sh2b1 (Myc-DDK-tagged ORF) - Rat SH2-B PH domain containing signaling mediator 1 (Sh2bpsm1), transcript variant 2, (10 ug)
"NM_001048180" in other vectors (3)
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CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Sh2-b; Sh2b; Sh2bpsm1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200245 representing NM_001048180
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGTGCCCCTTCCCCAGAGGATGGGGTTTTCCCTTCTCCACCAGCGCTGCCACCACCCCCTCCCC CAAGTTGGCAAGAGTTCTGTGAGTCCCATGCGAGGGCTGCTGCCCTGGATCTTGCTCGCCGTTTTCGCCT CTATCTGGCCTCCCACCCACAATATGCAGAGCCGGGAGCAGAGGCTGCCTTTTCTGGCCGTTTTGCTGAG CTCTTCCTGCAGCACTTCGAAGCTGAGGTGGCTCGGGCCTCGGGCTCACTCTCCCCACCTGTCTTGGCTC CATTGAGCCCTGGTGTGGAAATCCCACCATCACATGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCC CCTGGCAGTGTTGGGCCCTTCTCGATCTTCTGAGGACCTGGCTGGCCCCCTTCCTTCCTCAGTCTCTTCC TCTACAACGTCCTCAAAGCCGAAGCTCAAGAAACGCTTCTCCCTCCGCTCGGTGGGTCGTTCAGTCAGAG GTTCTGTCCGAGGCATCCTGCAGTGGCGGGGGGCTGTTGAATCTCCCTCCCAAGCTGGGCCTCTGGAGAC CACATCAGGTCCTCCAGTTCTAGGTGGAAACAGCAACTCCAACTCCTCTGGTGGTGCTGGGACAGTTGGT AGGGCATTGGCCAACGATGGCACATCCCCTGGGGAGAGATGGACTCATCGCTTTGAGAGGCTAAGGCTAA GTCGTGGAGGGGGAACCTTGAGAGACGGAGCAGGAGTGATACAGAGAGAAGAGCTGCTGAGTTTCATGGG GGCTGAAGAGGCTGCCCCTGACCCAGCAGGAGTAGGTCGTGGAGGAGGGGCAGCTGGGCTGACCTCGGGA GGAGGAGGGCAGCCTCAGTGGCAGAAATGTCGATTACTGCTCCGGAGTGAAGGAGAAGGAGGAGGAGGAA GTCGCTTGGAGTTCTTTGTACCACCCAAGGCATCCCGGCCCCGTCTTAGCATTCCCTGTTCTACTATTAC TGATGTCCGCACAGCCACAGCCCTGGAGATGCCTGACAGGGAGAACACGTTTGTGGTTAAGGTAGAAGGC CCTTCAGAGTACATCCTGGAGACAACTGATGCACTTCATGTGAAGGCCTGGGTGTCTGACATCCAAGAGT GCCTAAGCCCAGGACCCTGCCCTGCTATCAGCCCCCGTCCCATGACCCTTCCCCTGGCCCCTGGGACCTC CTTCCTCACAAAGGATAACACAGAGAGCCTGGAGTTGCCCTGCCTGAATCATTCAGAGAGTCTGCCTAGC CAGGATCTTCTTCTGGGACCCAGCGAGAGTAACGACCGCCTGTCGCAGGGAGCTTATGGAGGCCTCTCAG ACCGGCCGTCAGCGTCCTTCTCCCCTAGTTCTGCCTCCATTGCTGCTTCCCATTTTGACTCAATGGAACT GCTTCCTCCAGAGTTGCCCCCTCGGATTCCCATTGAGGAGGGGCCTCCAGCAGGGACAGTTCATCCCCTC TCTACCCCGTACCCTCCCCTGGATACTCCTGAAGCAGCCACAGGGTCATTCCTCTTTCAAGGGGAGGCAG AGGGGGGTGAGGGGGACCAGCCCCTCTCAGGCTACCCTTGGTTCCACGGCATGCTCTCTCGGCTCAAAGC TGCCCAGTTAGTGTTAGAAGGAGGTACCAGCTCCCATGGTGTCTTCTTGGTACGCCAGAGTGAGACAAGA CGTGGTGAATATGTCCTCACTTTCAACTTCCAGGGCAAGGCTAAGCACCTGCGTTTGTCACTAAATGAGG AGGGTCAGTGCCGGGTCCAACATCTGTGGTTCCAGTCCATTTTCGATATGCTTGAGCACTTCCGGGTGCA CCCCATCCCTCTGGAGTCTGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTGCCCTCCCAGCGGCAG CAGGGCCGGGAGCAGGCTGGGAGCCATGCAGGGGTGTGCGAGGGCGACCGATGCTACCCCGATGCCTCCT CCACCCTCCTGCCCTTCGGAGCGAGTGACTGTGTAACGGAGCACCTCCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200245 representing NM_001048180
Red=Cloning site Green=Tags(s) MNGAPSPEDGVFPSPPALPPPPPPSWQEFCESHARAAALDLARRFRLYLASHPQYAEPGAEAAFSGRFAE LFLQHFEAEVARASGSLSPPVLAPLSPGVEIPPSHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVSS STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGAVESPSQAGPLETTSGPPVLGGNSNSNSSGGAGTVG RALANDGTSPGERWTHRFERLRLSRGGGTLRDGAGVIQREELLSFMGAEEAAPDPAGVGRGGGAAGLTSG GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSTITDVRTATALEMPDRENTFVVKVEG PSEYILETTDALHVKAWVSDIQECLSPGPCPAISPRPMTLPLAPGTSFLTKDNTESLELPCLNHSESLPS QDLLLGPSESNDRLSQGAYGGLSDRPSASFSPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPL STPYPPLDTPEAATGSFLFQGEAEGGEGDQPLSGYPWFHGMLSRLKAAQLVLEGGTSSHGVFLVRQSETR RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSQRQ QGREQAGSHAGVCEGDRCYPDASSTLLPFGASDCVTEHLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001048180 |
| ORF Size | 2010 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001048180.1, NP_001041645.1 |
| RefSeq Size | 2791 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 89817 |
| UniProt ID | Q62985 |
| MW | 70.9 kDa |
| Gene Summary | interacts with kinase-active tyrosyl-phosphorylated JAK2 [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200245 | Sh2b1 (untagged ORF) - Rat SH2-B PH domain containing signaling mediator 1 (Sh2bpsm1), transcript variant 2, (10 ug) |
CNY 8,650.00 |
|
| RR200245L3 | Lenti ORF clone of Sh2b1 (Myc-DDK-tagged ORF) - Rat SH2-B PH domain containing signaling mediator 1 (Sh2bpsm1), transcript variant 2, (10 ug) |
CNY 10,450.00 |
|
| RR200245L4 | Lenti ORF clone of Sh2b1 (mGFP-tagged ORF) - Rat SH2-B PH domain containing signaling mediator 1 (Sh2bpsm1), transcript variant 2, (10 ug) |
CNY 11,500.00 |
