Trpm1 (NM_001037733) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200070 representing NM_001037733 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCCATGAGGAAAATGAGCAGCTCCTTCAAGCGTGGTTCAATCAAGAGCTCCACATCAGGGTCCC AGAAGGGTCAGAAAGCATGGATAGAGAAGACGTTTTGCAAAAGGGAATGCATCTTCGTGATTCCTAGTAC AAAAGACCCGAACAGATGTTGCTGTGGTCAGCTCACTAACCAGCACATCCCCCCGCTGCCGAGTGTGACT CCCAGCTCAACAGCAGAGGACACCAAGCAAGGGGACGCACAGTCCGGGAAATGGTCTGTCAGCAAACACA CCCAGAGCTACCCAACAGACTCCTATGGGATTCTTGAATTCCAGGGTGGGGGCTACTCCAATAAAGCCAT GTATATCCGAGTCTCCTACGACACCAAGCCAGATTCTCTGCTCCATCTCATGGTGAAGGACTGGCAGCTG GAGCTCCCCAAGCTCTTGATATCTGTGCATGGAGGCCTCCAGAGCTTTGAGATGCAGCCCAAGCTGAAGC AGGTCTTTGGGAAAGGTCTGATCAAGGCTGCCATGACCACAGGGGCATGGATCTTCACCGGGGGAGTGAG CACTGGTGTCGTCAGCCATGTGGGGGATGCCTTGAAGGACCACTCCTCCAAGTCCAGAGGCCGGCTCTGT GCTATCGGAATCGCTCCCTGGGGCATGGTGGAGAACAAGGAAGATCTGGTTGGGAAAGATGTAACGAGAG TCTACCAGACCATGTCCAACCCTCTGAGCAAGCTCTCCGTGCTCAACAATTCTCACACTCACTTCATCTT GGCTGACAATGGCACCCTTGGCAAGTATGGTGCCGAGGTGAAGCTTCGAAGACAACTGGAAAAACACATC TCCCTGCAGAAGATCAACACGAGGCTGGGCCAGGGTGTACCTGTCGTGGGTCTCGTGGTGGAAGGTGGAC CTAATGTGGTTTCTATCGTCCTGGAGTATCTCAGAGAAGACCCTCCTGTCCCTGTGGTGGTTTGTGACGG CAGTGGACGCGCCTCCGACATTTTGTCTTTTGCACACAAATACTGCGACGAAGGAGGGGTCATAAACGAG TCCCTGCGGGACCAGCTTCTAGTTACCATTCAGAAAACATTTAATTACAGCAAGTCCCAGTCGCATCAGC TGTTTGCAATTATCATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTTCGGATGGGCTCTGAGGG TCAGCAAGATGTTGAGATGGCAATTTTAACTGCCTTGCTCAAAGGAACCAACGTGTCAGCTCCAGATCAG CTGAGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTCGTCTTTGGCCCACACT GGCCGCCACTGGGAAGCCTGGCCCCTCCTGTGGACACCAAAGTCGCAGAGAAGGAGAAGAAGCCACCCAC AGCCACCACCAAGGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGCAAAGTGAAAGAGGAGGTGGAGGAA GAAACAGACCCCCGGAAGATCGAGCTGCTGAACTGGGTGAACGCCCTGGAGCAAGCCATGCTGGACGCTC TTGTCTTAGACCGGGTTGACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAGCATTTCCTCAC TATCCCGAGGCTGGAGGAGCTTTACAACACTAGACTGGGCCCACCAAACACCCTTCATCTGCTGGTGCGG GATGTGAAGAAGAGCAACCTTCCACCTGACTACCACATCAGCCTCATCGATATAGGACTGGTGTTGGAGT ACCTCATGGGAGGTGCCTACCGCTGCAACTACACTCGGAAAAGCTTCCGGACTCTCTACAACAACTTGTT TGGCCCTAAGAGGCCAAAAGCACTTAAACTCCTGGGAATGGAAGATGATGAGCCCCCAGCCAAGGGGAAG AAGAAAAAGAAGAAGAAGAAAGAGGAGGAGATTGATATAGATGTGGACGACCCCGCTGTGAGTCGGTTCC AGTATCCGTTCCACGAGCTCATGGTATGGGCCGTGCTCATGAAGCGCCAGAAGATGGCAGTCTTCCTGTG GCAGCGTGGGGAGGAGTGCATGGCAAAGGCCTTGGTGGCCTGTAAGCTCTACAAGGCCATGGCCCACGAG TCCTCGGAGAGCGAGCTGGTGGATGACATCTCCCAGGATCTGGACAACAATTCCAAGGATTTTGGCCAGC TCGCTGTGGAGCTGTTAGACCAGTCTTACAAGCACGATGAGCAAGTGGCCATGAAACTTCTGACCTATGA GCTCAAGAACTGGAGCAACTCGACCTGCCTTAAGCTGGCCGTGGCGGCCAAGCACAGGGACTTTATCGCT CACACCTGCAGCCAGATGCTGCTGACAGACATGTGGATGGGAAGGCTTCGCATGAGGAAGAACCCTGGCC TGAAGGTCATCATGGGGATCCTTATCCCGCCCACCATCTTGTTTTTGGAATTCCGTTCATATGATGACTT CTCCTATCAAACATCCAAGGAGAATGAAGATGGCAAAGAAAAGGAAGAAGAGAATGTGGATGCAAATGCA GATGCTGGCTCAAGAAAGGGGGATGAGGAGAATGAGCACAAAAAACAGAGGAGCATTCCCATTGGAACAA AGATCTGTGAATTCTATAATGCGCCCATTGTCAAGTTCTGGTTTTACACAATCTCCTACTTGGGCTACTT GCTGCTGTTCAATTATGTCATCCTGGTGCGGATGGACGGCTGGCCTTCACCCCAAGAATGGATCGTTATC TCCTACATTGTGAGCCTGGCACTAGAGAAAATACGAGAGATCCTCATGTCGGAACCAGGCAAACTCAGCC AGAAAATCAAAGTGTGGCTCCAAGAGTACTGGAACATCACAGACCTTGTGGCCATTTCCATGTTCATGGT TGGGGCCATCCTTCGCCTCCAGAACCAGCCATACATGGGCTATGGCCGGGTCATCTACTGTGTGGATATC ATCCTCTGGTACATCCGAGTGTTAGACATCTTTGGTGTCAACAAGTATCTTGGCCCCTATGTAATGATGA TTGGAAAGATGATGATTGACATGCTGTACTTTGTGGTCATTATGCTGGTTGTGCTGATGAGTTTCGGCGT AGCTCGGCAAGCCATCTTGCACCCAGAGGAGAAGCCTTCTTGGAAACTGGCCCGAAACATCTTCTACATG CCCTACTGGATGATCTACGGAGAAGTGTTTGCAGACCAGATAGACCTCTATGCCATGGAAATCAACCCTC CTTGTGGTGAAAATCTCTATGATGAGGAAGGCAAGAGGCTCCCTCCCTGCATCCCTGGTGCCTGGCTCAC ACCTGCCCTCATGGCCTGTTATCTCCTGGTGGCCAATATCCTGCTGGTCAACCTGCTGATTGCTGTTTTC AACAATACCTTCTTTGAAGTCAAGTCAATATCCAATCAAGTGTGGAAGTTCCAGCGGTACCAACTGATCA TGACATTTCACGACAGGCCGGTCCTGCCCCCGCCAATGATCATCTTAAGCCACATCTACATCATCGTTAT GCGTCTCAGTGGTCGCTGCAGAAAAAAGAGAGAAGGGGACCAGGAGGAGCGGGATCGTGGGTTGAAGCTG TTCCTCAGCGACGAGGAACTGAAAAAACTGCATGAGTTTGAGGAGCAGTGCGTGCAGGAGCACTTCCGAG AAAAGGAGGATGAGCAGCAGTCGTCCAGCGATGAGCGCATCCGGGTTACAAGCGAGAGAGTTGAAAATAT GTCAATGAGGCTGGAGGAAATCAACGAAAGAGAAAATTTCATGAAGGCGTCTCTGCAGACTGTCGACCTT CGGCTCTCTCAGCTGGAGGAGCTATCTGGCAGGATGGTGGGCGCTCTGGAGAATCTGGCAGGCATCGACA GGTCTGATCTGATCCAGGCTCGATCAAGAGCATCCTCAGAATGTGAGGCCACCTACCTGCTGCGGCAGAG CAGTATCAACAGCGCCGATGGCTACAGCATGTACCGCTACCATTTCAATGGCGAGGAGCTTCTGTTTGAG GAGCCCGCCCTCTCCACTTCGCCAGGGACAGTGTTCCGGAAAAAAACCTGCTCCTTCCGCGTGAAGGAAG AGGATGTAAAACCACACCTGGACCAGCCAAGTAGCCTGCACCACACCCCAGGCCCCAGCCCGCCTGCCAC GCCAGGTCGCAGCCGGCTTGCCCTTGATGGTCCCTTGAGCACAGAGTTGAGACCTGGACTGGATCCTGGT ATCTCTGCGGGTGAGCTTGATCCGCGGGCAGATTTCAAGAGCGCCGAAGTTGCACCAAGTCTGAACACGG CGAATGTTGCAAGCACTCAGCTGACCGTCGAAAGCACTGTTTCCCACCCACTGCGGGAGAGCAAACTCGC GCGCTACTACCCCGGAGACCTCAACACCTACAAAACAATGAAGTCCAGAAGCTTCGTCTATTCTGAAGGA AGAAAACTTGTCCGAGGTCTTAGCAACTGGGGTGCCGAGTACAGTTCAATCATGGACCAAACATGGAACT CGGCAGAATGGAGATGTCAAGTTCAAAGGATCACACGCTCCCGCAGCACAGACATCCCGTACATTGTGTC TGAAGCAGCCTCCCAAGATGAGTTTGAGGATGAGCACAGAGAATCTCTTCTGGCTCCTCAGATCTCCCGT TCAGCCCTCACGGTCTCTGACAGGCCCGAAAAGGAAAACTTGCTGTCTGTGAAGCCACACCAGACTTTAG GATTCCCCTGCCTACGGTCAAGAAGTTTACATGGTCATCCTAGGAGTGCCAAACCCTCTCCTAGCAAATT AGACAGGGCGGGACATGCCAGCAGCACCAGCAACTTAGCAGTTATGTCAGACGCTCCAGAGGGACAAAAC ACCCAGCAGGAGAAAGGAAACCCGGAAACTGAATGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200070 representing NM_001037733 Red=Cloning site Green=Tags(s) MGSMRKMSSSFKRGSIKSSTSGSQKGQKAWIEKTFCKRECIFVIPSTKDPNRCCCGQLTNQHIPPLPSVT PSSTAEDTKQGDAQSGKWSVSKHTQSYPTDSYGILEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQL ELPKLLISVHGGLQSFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGVSTGVVSHVGDALKDHSSKSRGRLC AIGIAPWGMVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRQLEKHI SLQKINTRLGQGVPVVGLVVEGGPNVVSIVLEYLREDPPVPVVVCDGSGRASDILSFAHKYCDEGGVINE SLRDQLLVTIQKTFNYSKSQSHQLFAIIMECMKKKELVTVFRMGSEGQQDVEMAILTALLKGTNVSAPDQ LSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPVDTKVAEKEKKPPTATTKGRGKGKGKKKGKVKEEVEE ETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVR DVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKSFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGK KKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVFLWQRGEECMAKALVACKLYKAMAHE SSESELVDDISQDLDNNSKDFGQLAVELLDQSYKHDEQVAMKLLTYELKNWSNSTCLKLAVAAKHRDFIA HTCSQMLLTDMWMGRLRMRKNPGLKVIMGILIPPTILFLEFRSYDDFSYQTSKENEDGKEKEEENVDANA DAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSPQEWIVI SYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISMFMVGAILRLQNQPYMGYGRVIYCVDI ILWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYM PYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVF NNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYIIVMRLSGRCRKKREGDQEERDRGLKL FLSDEELKKLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERENFMKASLQTVDL RLSQLEELSGRMVGALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSMYRYHFNGEELLFE EPALSTSPGTVFRKKTCSFRVKEEDVKPHLDQPSSLHHTPGPSPPATPGRSRLALDGPLSTELRPGLDPG ISAGELDPRADFKSAEVAPSLNTANVASTQLTVESTVSHPLRESKLARYYPGDLNTYKTMKSRSFVYSEG RKLVRGLSNWGAEYSSIMDQTWNSAEWRCQVQRITRSRSTDIPYIVSEAASQDEFEDEHRESLLAPQISR SALTVSDRPEKENLLSVKPHQTLGFPCLRSRSLHGHPRSAKPSPSKLDRAGHASSTSNLAVMSDAPEGQN TQQEKGNPETEC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001037733
ORF Size	4866 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001037733.1, NP_001032822.1
RefSeq Size	5012 bp
RefSeq ORF	4869 bp
Locus ID	361586
UniProt ID	Q2WEA5
MW	183.5 kDa
Gene Summary	Calcium channel which may play a role in metastasis suppression. May act as a spontaneously active, calcium-permeable plasma membrane channel (By similarity).[UniProtKB/Swiss-Prot Function]

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